SgRNA Expression of CRIPSR-Cas9 System Based on MiRNA Polycistrons as a Versatile Tool to Manipulate Multiple and Tissue-Specific Genome Editing

CRISPR/Cas9-mediated genome editing is a next-generation strategy for genetic modifications. Typically, sgRNA is constitutively expressed relying on RNA polymerase III promoters. Polymerase II promoters initiate transcription in a flexible manner, but sgRNAs generated by RNA polymerase II promoter l...

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Detalles Bibliográficos
Autores principales: Xie, Chen, Chen, Yan-Lian, Wang, Dong-Fang, Wang, Yi-Lin, Zhang, Tian-Peng, Li, Hui, Liang, Fu, Zhao, Yong, Zhang, Guang-Ya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5517485/
https://www.ncbi.nlm.nih.gov/pubmed/28724960
http://dx.doi.org/10.1038/s41598-017-06216-w

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