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Centrosomal protein 55 activates NF-κB signalling and promotes pancreatic cancer cells aggressiveness
Centrosomal protein 55 (CEP55) is a microtubule-bundling protein that participants in cell mitosis. It is overexpressed in several solid tumours and promotes the growth and invasion of cancer cells. However, the role of CEP55 in pancreatic cancer (PANC) remains unclear. Herein, upregulated expressio...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5517556/ https://www.ncbi.nlm.nih.gov/pubmed/28724890 http://dx.doi.org/10.1038/s41598-017-06132-z |
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author | Peng, Tao Zhou, Wei Guo, Feng Wu, He-shui Wang, Chun-you Wang, Li Yang, Zhi-yong |
author_facet | Peng, Tao Zhou, Wei Guo, Feng Wu, He-shui Wang, Chun-you Wang, Li Yang, Zhi-yong |
author_sort | Peng, Tao |
collection | PubMed |
description | Centrosomal protein 55 (CEP55) is a microtubule-bundling protein that participants in cell mitosis. It is overexpressed in several solid tumours and promotes the growth and invasion of cancer cells. However, the role of CEP55 in pancreatic cancer (PANC) remains unclear. Herein, upregulated expression of CEP55 (associated with poor prognosis) was detected in PANC using quantitative real-time reverse transcription PCR, western blotting, and immunohistochemistry. Cell migration, colony formation, wound-healing, and Transwell matrix penetration assays, revealed that upregulation of CEP55 promoted PANC cells proliferation, migration, and invasion in vitro, whereas knockdown of CEP55 attenuated it. In an in vivo murine model, CEP55 overexpression accelerated PANC cells tumourigenicity, together with upregulation of the protein levels of invasion-related proteins matrix metalloproteinase (MMP)2, MMP9, and proliferation-related protein Cyclin D1. Downregulation of CEP55 had the reverse effect. Moreover, the nuclear factor κB (NF-κB)/IκBα signalling pathway, which was activated in CEP55-transduced PANC cells and inhibited in CEP55-silenced PANC cells, contributed to CEP55-mediated PANC cell aggressiveness. This study provided new insights into the oncogenic roles of CEP55 and the mechanism by which the NF-κB pathway is hyperactivated in patients with PANC, indicating that CEP55 is a valuable prognostic factor and a potential therapeutic target in PANC. |
format | Online Article Text |
id | pubmed-5517556 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55175562017-07-20 Centrosomal protein 55 activates NF-κB signalling and promotes pancreatic cancer cells aggressiveness Peng, Tao Zhou, Wei Guo, Feng Wu, He-shui Wang, Chun-you Wang, Li Yang, Zhi-yong Sci Rep Article Centrosomal protein 55 (CEP55) is a microtubule-bundling protein that participants in cell mitosis. It is overexpressed in several solid tumours and promotes the growth and invasion of cancer cells. However, the role of CEP55 in pancreatic cancer (PANC) remains unclear. Herein, upregulated expression of CEP55 (associated with poor prognosis) was detected in PANC using quantitative real-time reverse transcription PCR, western blotting, and immunohistochemistry. Cell migration, colony formation, wound-healing, and Transwell matrix penetration assays, revealed that upregulation of CEP55 promoted PANC cells proliferation, migration, and invasion in vitro, whereas knockdown of CEP55 attenuated it. In an in vivo murine model, CEP55 overexpression accelerated PANC cells tumourigenicity, together with upregulation of the protein levels of invasion-related proteins matrix metalloproteinase (MMP)2, MMP9, and proliferation-related protein Cyclin D1. Downregulation of CEP55 had the reverse effect. Moreover, the nuclear factor κB (NF-κB)/IκBα signalling pathway, which was activated in CEP55-transduced PANC cells and inhibited in CEP55-silenced PANC cells, contributed to CEP55-mediated PANC cell aggressiveness. This study provided new insights into the oncogenic roles of CEP55 and the mechanism by which the NF-κB pathway is hyperactivated in patients with PANC, indicating that CEP55 is a valuable prognostic factor and a potential therapeutic target in PANC. Nature Publishing Group UK 2017-07-19 /pmc/articles/PMC5517556/ /pubmed/28724890 http://dx.doi.org/10.1038/s41598-017-06132-z Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Peng, Tao Zhou, Wei Guo, Feng Wu, He-shui Wang, Chun-you Wang, Li Yang, Zhi-yong Centrosomal protein 55 activates NF-κB signalling and promotes pancreatic cancer cells aggressiveness |
title | Centrosomal protein 55 activates NF-κB signalling and promotes pancreatic cancer cells aggressiveness |
title_full | Centrosomal protein 55 activates NF-κB signalling and promotes pancreatic cancer cells aggressiveness |
title_fullStr | Centrosomal protein 55 activates NF-κB signalling and promotes pancreatic cancer cells aggressiveness |
title_full_unstemmed | Centrosomal protein 55 activates NF-κB signalling and promotes pancreatic cancer cells aggressiveness |
title_short | Centrosomal protein 55 activates NF-κB signalling and promotes pancreatic cancer cells aggressiveness |
title_sort | centrosomal protein 55 activates nf-κb signalling and promotes pancreatic cancer cells aggressiveness |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5517556/ https://www.ncbi.nlm.nih.gov/pubmed/28724890 http://dx.doi.org/10.1038/s41598-017-06132-z |
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