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Saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community

The oral cavity harbours a complex microbiome that is linked to dental diseases and serves as a route to other parts of the body. Here, the aims were to characterize the oral microbiota by deep sequencing in a low-caries population with regular dental care since childhood and search for association...

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Autores principales: Eriksson, Linda, Lif Holgerson, Pernilla, Johansson, Ingegerd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5517611/
https://www.ncbi.nlm.nih.gov/pubmed/28724921
http://dx.doi.org/10.1038/s41598-017-06221-z
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author Eriksson, Linda
Lif Holgerson, Pernilla
Johansson, Ingegerd
author_facet Eriksson, Linda
Lif Holgerson, Pernilla
Johansson, Ingegerd
author_sort Eriksson, Linda
collection PubMed
description The oral cavity harbours a complex microbiome that is linked to dental diseases and serves as a route to other parts of the body. Here, the aims were to characterize the oral microbiota by deep sequencing in a low-caries population with regular dental care since childhood and search for association with caries prevalence and incidence. Saliva and tooth biofilm from 17-year-olds and mock bacteria communities were analysed using 16S rDNA Illumina MiSeq (v3-v4) and PacBio SMRT (v1-v8) sequencing including validity and reliability estimates. Caries was scored at 17 and 19 years of age. Both sequencing platforms revealed that Firmicutes dominated in the saliva, whereas Firmicutes and Actinobacteria abundances were similar in tooth biofilm. Saliva microbiota discriminated caries-affected from caries-free adolescents, with enumeration of Scardovia wiggsiae, Streptococcus mutans, Bifidobacterium longum, Leptotrichia sp. HOT498, and Selenomonas spp. in caries-affected participants. Adolescents with B. longum in saliva had significantly higher 2-year caries increment. PacBio SMRT revealed Corynebacterium matruchotii as the most prevalent species in tooth biofilm. In conclusion, both sequencing methods were reliable and valid for oral samples, and saliva microbiota was associated with cross-sectional caries prevalence, especially S. wiggsiae, S. mutans, and B. longum; the latter also with the 2-year caries incidence.
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spelling pubmed-55176112017-07-20 Saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community Eriksson, Linda Lif Holgerson, Pernilla Johansson, Ingegerd Sci Rep Article The oral cavity harbours a complex microbiome that is linked to dental diseases and serves as a route to other parts of the body. Here, the aims were to characterize the oral microbiota by deep sequencing in a low-caries population with regular dental care since childhood and search for association with caries prevalence and incidence. Saliva and tooth biofilm from 17-year-olds and mock bacteria communities were analysed using 16S rDNA Illumina MiSeq (v3-v4) and PacBio SMRT (v1-v8) sequencing including validity and reliability estimates. Caries was scored at 17 and 19 years of age. Both sequencing platforms revealed that Firmicutes dominated in the saliva, whereas Firmicutes and Actinobacteria abundances were similar in tooth biofilm. Saliva microbiota discriminated caries-affected from caries-free adolescents, with enumeration of Scardovia wiggsiae, Streptococcus mutans, Bifidobacterium longum, Leptotrichia sp. HOT498, and Selenomonas spp. in caries-affected participants. Adolescents with B. longum in saliva had significantly higher 2-year caries increment. PacBio SMRT revealed Corynebacterium matruchotii as the most prevalent species in tooth biofilm. In conclusion, both sequencing methods were reliable and valid for oral samples, and saliva microbiota was associated with cross-sectional caries prevalence, especially S. wiggsiae, S. mutans, and B. longum; the latter also with the 2-year caries incidence. Nature Publishing Group UK 2017-07-19 /pmc/articles/PMC5517611/ /pubmed/28724921 http://dx.doi.org/10.1038/s41598-017-06221-z Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Eriksson, Linda
Lif Holgerson, Pernilla
Johansson, Ingegerd
Saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community
title Saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community
title_full Saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community
title_fullStr Saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community
title_full_unstemmed Saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community
title_short Saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community
title_sort saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5517611/
https://www.ncbi.nlm.nih.gov/pubmed/28724921
http://dx.doi.org/10.1038/s41598-017-06221-z
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