Cargando…

Novel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq data

Normalization of high-throughput small RNA sequencing (sRNA-Seq) data is required to compare sRNA levels across different samples. Commonly used relative normalization approaches can cause erroneous conclusions due to fluctuating small RNA populations between tissues. We developed a set of sRNA spik...

Descripción completa

Detalles Bibliográficos
Autores principales:	Lutzmayer, Stefan, Enugutti, Balaji, Nodine, Michael D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5517642/ https://www.ncbi.nlm.nih.gov/pubmed/28724941 http://dx.doi.org/10.1038/s41598-017-06174-3

Ejemplares similares

Chromatin regulates expression of small RNAs to help maintain transposon methylome homeostasis in Arabidopsis
por: Papareddy, Ranjith K., et al.
Publicado: (2020)

Evaluation of normalization procedures for oligonucleotide array data based on spiked cRNA controls
por: Hill, Andrew A, et al.
Publicado: (2001)

Improving small RNA-seq by using a synthetic spike-in set for size-range quality control together with a set for data normalization
por: Locati, Mauro D., et al.
Publicado: (2015)

The YTHDF proteins ECT2 and ECT3 bind largely overlapping target sets and influence target mRNA abundance, not alternative polyadenylation
por: Arribas-Hernández, Laura, et al.
Publicado: (2021)

Normalization of human RNA-seq experiments using chimpanzee RNA as a spike-in standard
por: Yu, Hannah, et al.
Publicado: (2016)

Deep annotation of long noncoding RNAs by assembling RNA-seq and small RNA-seq data
por: Zhang, Jiaming, et al.
Publicado: (2023)

Prediction of Novel Bacterial Small RNAs From RIL-Seq RNA–RNA Interaction Data
por: Bar, Amir, et al.
Publicado: (2021)

RNA-Seq Signatures Normalized by mRNA Abundance Allow Absolute Deconvolution of Human Immune Cell Types
por: Monaco, Gianni, et al.
Publicado: (2019)

Gene expression variation in Arabidopsis embryos at single-nucleus resolution
por: Kao, Ping, et al.
Publicado: (2021)

Oasis 2: improved online analysis of small RNA-seq data
por: Rahman, Raza-Ur, et al.
Publicado: (2018)

Bookend: precise transcript reconstruction with end-guided assembly
por: Schon, Michael A., et al.
Publicado: (2022)

Publisher Correction: Bookend: precise transcript reconstruction with end-guided assembly
por: Schon, Michael A., et al.
Publicado: (2022)

Antisense Oligonucleotides and Small Interfering RNA for the Treatment of Dyslipidemias
por: Gareri, Clarice, et al.
Publicado: (2022)

LSH-GAN enables in-silico generation of cells for small sample high dimensional scRNA-seq data
por: Lall, Snehalika, et al.
Publicado: (2022)

shortran: a pipeline for small RNA-seq data analysis
por: Gupta, Vikas, et al.
Publicado: (2012)

Antagonistic activities of cotranscriptional regulators within an early developmental window set FLC expression level
por: Schon, Michael, et al.
Publicado: (2021)

GC-Content Normalization for RNA-Seq Data
por: Risso, Davide, et al.
Publicado: (2011)

An integrative method to normalize RNA-Seq data
por: Filloux, Cyril, et al.
Publicado: (2014)

An Integrated Approach for RNA-seq Data Normalization
por: Yang, Shengping, et al.
Publicado: (2016)

Droplet Digital Enzyme-Linked Oligonucleotide Hybridization Assay for Absolute RNA Quantification
por: Guan, Weihua, et al.
Publicado: (2015)

Exploration of small RNA-seq data for small non-coding RNAs in Human Colorectal Cancer
por: Koduru, Srinivas V, et al.
Publicado: (2017)

Cross-platform normalization enables machine learning model training on microarray and RNA-seq data simultaneously
por: Foltz, Steven M., et al.
Publicado: (2023)

COMPSRA: a COMprehensive Platform for Small RNA-Seq data Analysis
por: Li, Jiang, et al.
Publicado: (2020)

Using Small RNA-seq Data to Detect siRNA Duplexes Induced by Plant Viruses
por: Niu, Xiaoran, et al.
Publicado: (2017)

The Impact of Normalization Methods on RNA-Seq Data Analysis
por: Zyprych-Walczak, J., et al.
Publicado: (2015)

iMir: An integrated pipeline for high-throughput analysis of small non-coding RNA data obtained by smallRNA-Seq
por: Giurato, Giorgio, et al.
Publicado: (2013)

Elimination of PCR duplicates in RNA-seq and small RNA-seq using unique molecular identifiers
por: Fu, Yu, et al.
Publicado: (2018)

Single-cell RNA-seq with spike-in cells enables accurate quantification of cell-specific drug effects in pancreatic islets
por: Marquina-Sanchez, Brenda, et al.
Publicado: (2020)

Improving Gene-Set Enrichment Analysis of RNA-Seq Data with Small Replicates
por: Yoon, Sora, et al.
Publicado: (2016)

Small RNA-seq reveals novel regulatory components for apomixis in Paspalum notatum
por: Ortiz, Juan Pablo A., et al.
Publicado: (2019)

SCnorm: robust normalization of single-cell RNA-seq data
por: Bacher, Rhonda, et al.
Publicado: (2017)

A graph-based algorithm for RNA-seq data normalization
por: Tran, Diem-Trang, et al.
Publicado: (2020)

MicroRNAs Involved in Regulatory Cytoplasmic Male Sterility by Analysis RNA-seq and Small RNA-seq in Soybean
por: Zhang, Chunbao, et al.
Publicado: (2021)

Functional evaluation of LTR-derived lncRNAs in porcine oocytes and zygotes with RNA-seq and small RNA-seq
por: Yang, Xu, et al.
Publicado: (2022)

miRge - A Multiplexed Method of Processing Small RNA-Seq Data to Determine MicroRNA Entropy
por: Baras, Alexander S., et al.
Publicado: (2015)

Removing bias against short sequences enables northern blotting to better complement RNA-seq for the study of small RNAs
por: Choi, Yun S., et al.
Publicado: (2017)

A novel computational method enables RNA editome profiling during human hematopoiesis from scRNA-seq data
por: Wu, Yan, et al.
Publicado: (2023)

RNA-Seq quantification of the human small airway epithelium transcriptome
por: Hackett, Neil R, et al.
Publicado: (2012)

SPAR: small RNA-seq portal for analysis of sequencing experiments
por: Kuksa, Pavel P, et al.
Publicado: (2018)

Quantitative mapping of the cellular small RNA landscape with AQRNA-seq
por: Hu, Jennifer F., et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_dlls2oo81m5o649l0f4fobv4l2