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Lactobacillus paracasei metabolism of rice bran reveals metabolome associated with Salmonella Typhimurium growth reduction

AIMS: This study aimed to determine the effect of a cell‐free supernatant of Lactobacillus paracasei ATCC 27092 with and without rice bran extract (RBE) on Salmonella Typhimurium 14028s growth, and to identify a metabolite profile with antimicrobial functions. METHODS AND RESULTS: Supernatant was co...

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Autores principales: Nealon, N.J., Worcester, C.R., Ryan, E.P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5518229/
https://www.ncbi.nlm.nih.gov/pubmed/28371001
http://dx.doi.org/10.1111/jam.13459
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author Nealon, N.J.
Worcester, C.R.
Ryan, E.P.
author_facet Nealon, N.J.
Worcester, C.R.
Ryan, E.P.
author_sort Nealon, N.J.
collection PubMed
description AIMS: This study aimed to determine the effect of a cell‐free supernatant of Lactobacillus paracasei ATCC 27092 with and without rice bran extract (RBE) on Salmonella Typhimurium 14028s growth, and to identify a metabolite profile with antimicrobial functions. METHODS AND RESULTS: Supernatant was collected from overnight cultures of L. paracasei incubated in the presence (LP+RBE) or absence (LP) of RBE and applied to S. Typhimurium. LP+RBE reduced 13·1% more S. Typhimurium growth than LP after 16 h (P < 0·05). Metabolite profiles of LP and LP+RBE were examined using nontargeted global metabolomics consisting of ultra‐high‐performance liquid chromatography coupled with tandem mass spectrometry. A comparison of LP and LP+RBE revealed 84 statistically significant metabolites (P < 0·05), where 20 were classified with antimicrobial functions. CONCLUSIONS: LP+RBE reduced S. Typhimurium growth to a greater extent than LP, and the metabolite profile distinctions suggested that RBE favourably modulates the metabolism of L. paracasei. These findings warrant continued investigation of probiotic and RBE antimicrobial activities across microenvironments and matrices where S. Typhimurium exposure is problematic. SIGNIFICANCE AND IMPACT OF THE STUDY: This study showed a novel metabolite profile of probiotic L. paracasei and prebiotic rice bran that increased antimicrobial activity against S. Typhimurium.
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spelling pubmed-55182292017-08-03 Lactobacillus paracasei metabolism of rice bran reveals metabolome associated with Salmonella Typhimurium growth reduction Nealon, N.J. Worcester, C.R. Ryan, E.P. J Appl Microbiol Original Articles AIMS: This study aimed to determine the effect of a cell‐free supernatant of Lactobacillus paracasei ATCC 27092 with and without rice bran extract (RBE) on Salmonella Typhimurium 14028s growth, and to identify a metabolite profile with antimicrobial functions. METHODS AND RESULTS: Supernatant was collected from overnight cultures of L. paracasei incubated in the presence (LP+RBE) or absence (LP) of RBE and applied to S. Typhimurium. LP+RBE reduced 13·1% more S. Typhimurium growth than LP after 16 h (P < 0·05). Metabolite profiles of LP and LP+RBE were examined using nontargeted global metabolomics consisting of ultra‐high‐performance liquid chromatography coupled with tandem mass spectrometry. A comparison of LP and LP+RBE revealed 84 statistically significant metabolites (P < 0·05), where 20 were classified with antimicrobial functions. CONCLUSIONS: LP+RBE reduced S. Typhimurium growth to a greater extent than LP, and the metabolite profile distinctions suggested that RBE favourably modulates the metabolism of L. paracasei. These findings warrant continued investigation of probiotic and RBE antimicrobial activities across microenvironments and matrices where S. Typhimurium exposure is problematic. SIGNIFICANCE AND IMPACT OF THE STUDY: This study showed a novel metabolite profile of probiotic L. paracasei and prebiotic rice bran that increased antimicrobial activity against S. Typhimurium. John Wiley and Sons Inc. 2017-05-11 2017-06 /pmc/articles/PMC5518229/ /pubmed/28371001 http://dx.doi.org/10.1111/jam.13459 Text en © 2017 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of The Society for Applied Microbiology. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Nealon, N.J.
Worcester, C.R.
Ryan, E.P.
Lactobacillus paracasei metabolism of rice bran reveals metabolome associated with Salmonella Typhimurium growth reduction
title Lactobacillus paracasei metabolism of rice bran reveals metabolome associated with Salmonella Typhimurium growth reduction
title_full Lactobacillus paracasei metabolism of rice bran reveals metabolome associated with Salmonella Typhimurium growth reduction
title_fullStr Lactobacillus paracasei metabolism of rice bran reveals metabolome associated with Salmonella Typhimurium growth reduction
title_full_unstemmed Lactobacillus paracasei metabolism of rice bran reveals metabolome associated with Salmonella Typhimurium growth reduction
title_short Lactobacillus paracasei metabolism of rice bran reveals metabolome associated with Salmonella Typhimurium growth reduction
title_sort lactobacillus paracasei metabolism of rice bran reveals metabolome associated with salmonella typhimurium growth reduction
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5518229/
https://www.ncbi.nlm.nih.gov/pubmed/28371001
http://dx.doi.org/10.1111/jam.13459
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