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Sphingolipids metabolism in the salivary glands of rats with obesity and streptozotocin induced diabetes

Diabetes is considered a major public health problem affecting millions of individuals worldwide. Remarkably, scientific reports regarding salivary glands sphingolipid metabolism in diabetes are virtually non‐existent. This is odd given the well‐established link between the both in other tissues (e....

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Autores principales: Garbowska, Marta, Łukaszuk, Bartłomiej, Mikłosz, Agnieszka, Wróblewski, Igor, Kurek, Krzysztof, Ostrowska, Lucyna, Chabowski, Adrian, Żendzian‐Piotrowska, Małgorzata, Zalewska, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5518447/
https://www.ncbi.nlm.nih.gov/pubmed/28369933
http://dx.doi.org/10.1002/jcp.25939
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author Garbowska, Marta
Łukaszuk, Bartłomiej
Mikłosz, Agnieszka
Wróblewski, Igor
Kurek, Krzysztof
Ostrowska, Lucyna
Chabowski, Adrian
Żendzian‐Piotrowska, Małgorzata
Zalewska, Anna
author_facet Garbowska, Marta
Łukaszuk, Bartłomiej
Mikłosz, Agnieszka
Wróblewski, Igor
Kurek, Krzysztof
Ostrowska, Lucyna
Chabowski, Adrian
Żendzian‐Piotrowska, Małgorzata
Zalewska, Anna
author_sort Garbowska, Marta
collection PubMed
description Diabetes is considered a major public health problem affecting millions of individuals worldwide. Remarkably, scientific reports regarding salivary glands sphingolipid metabolism in diabetes are virtually non‐existent. This is odd given the well‐established link between the both in other tissues (e.g., skeletal muscles, liver) and the key role of these glands in oral health preservation. The aim of this paper is to examine sphingolipids metabolism in the salivary glands in (pre)diabetes (evoked by high fat diet feeding or streptozotocin). Wistar rats were allocated into three groups: control, HFD‐, or STZ‐diabetes. The content of major sphingolipid classes in the parotid (PSG) and submandibular (SMSG) glands was assessed via chromatography. Additionally, Western blot analyses were employed for the evaluation of key sphingolipid signaling pathway enzyme levels. No changes in ceramide content in the PSG were found, whereas an increase in ceramide concentration for SMSG of the STZ group was observed. This was accompanied by an elevation in SPT1 level. Probably also sphingomyelin hydrolysis was increased in the SMSG of the STZ‐diabetic rats, since we observed a significant drop in the amount of SM. PSG and SMSG respond differently to (pre)diabetes, with clearer pattern presented by the later gland. An activation of sphingomyelin signaling pathway was observed in the course of STZ‐diabetes, that is, metabolic condition with rapid onset/progression. Whereas, chronic HFD lead to an inhibition of sphingomyelin signaling pathway in the salivary glands (manifested in an inhibition of ceramide de novo synthesis and accumulation of S1P).
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spelling pubmed-55184472017-08-03 Sphingolipids metabolism in the salivary glands of rats with obesity and streptozotocin induced diabetes Garbowska, Marta Łukaszuk, Bartłomiej Mikłosz, Agnieszka Wróblewski, Igor Kurek, Krzysztof Ostrowska, Lucyna Chabowski, Adrian Żendzian‐Piotrowska, Małgorzata Zalewska, Anna J Cell Physiol Original Research Articles Diabetes is considered a major public health problem affecting millions of individuals worldwide. Remarkably, scientific reports regarding salivary glands sphingolipid metabolism in diabetes are virtually non‐existent. This is odd given the well‐established link between the both in other tissues (e.g., skeletal muscles, liver) and the key role of these glands in oral health preservation. The aim of this paper is to examine sphingolipids metabolism in the salivary glands in (pre)diabetes (evoked by high fat diet feeding or streptozotocin). Wistar rats were allocated into three groups: control, HFD‐, or STZ‐diabetes. The content of major sphingolipid classes in the parotid (PSG) and submandibular (SMSG) glands was assessed via chromatography. Additionally, Western blot analyses were employed for the evaluation of key sphingolipid signaling pathway enzyme levels. No changes in ceramide content in the PSG were found, whereas an increase in ceramide concentration for SMSG of the STZ group was observed. This was accompanied by an elevation in SPT1 level. Probably also sphingomyelin hydrolysis was increased in the SMSG of the STZ‐diabetic rats, since we observed a significant drop in the amount of SM. PSG and SMSG respond differently to (pre)diabetes, with clearer pattern presented by the later gland. An activation of sphingomyelin signaling pathway was observed in the course of STZ‐diabetes, that is, metabolic condition with rapid onset/progression. Whereas, chronic HFD lead to an inhibition of sphingomyelin signaling pathway in the salivary glands (manifested in an inhibition of ceramide de novo synthesis and accumulation of S1P). John Wiley and Sons Inc. 2017-04-27 2017-10 /pmc/articles/PMC5518447/ /pubmed/28369933 http://dx.doi.org/10.1002/jcp.25939 Text en © 2017 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Articles
Garbowska, Marta
Łukaszuk, Bartłomiej
Mikłosz, Agnieszka
Wróblewski, Igor
Kurek, Krzysztof
Ostrowska, Lucyna
Chabowski, Adrian
Żendzian‐Piotrowska, Małgorzata
Zalewska, Anna
Sphingolipids metabolism in the salivary glands of rats with obesity and streptozotocin induced diabetes
title Sphingolipids metabolism in the salivary glands of rats with obesity and streptozotocin induced diabetes
title_full Sphingolipids metabolism in the salivary glands of rats with obesity and streptozotocin induced diabetes
title_fullStr Sphingolipids metabolism in the salivary glands of rats with obesity and streptozotocin induced diabetes
title_full_unstemmed Sphingolipids metabolism in the salivary glands of rats with obesity and streptozotocin induced diabetes
title_short Sphingolipids metabolism in the salivary glands of rats with obesity and streptozotocin induced diabetes
title_sort sphingolipids metabolism in the salivary glands of rats with obesity and streptozotocin induced diabetes
topic Original Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5518447/
https://www.ncbi.nlm.nih.gov/pubmed/28369933
http://dx.doi.org/10.1002/jcp.25939
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