Cargando…
miRNA-340 inhibits osteoclast differentiation via repression of MITF
Many miRNAs play critical roles in modulating various biological processes of osteoclast differentiation and function. Microphthalmia-associated transcription factor (MITF), a target of miR-340, served as pivotal transcription factor involved in osteoclast differentiation. However, the role of miR-3...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5518531/ https://www.ncbi.nlm.nih.gov/pubmed/28607030 http://dx.doi.org/10.1042/BSR20170302 |
_version_ | 1783251510399860736 |
---|---|
author | Zhao, Hongying Zhang, Jun Shao, Haiyu Liu, Jianwen Jin, Mengran Chen, Jinping Huang, Yazeng |
author_facet | Zhao, Hongying Zhang, Jun Shao, Haiyu Liu, Jianwen Jin, Mengran Chen, Jinping Huang, Yazeng |
author_sort | Zhao, Hongying |
collection | PubMed |
description | Many miRNAs play critical roles in modulating various biological processes of osteoclast differentiation and function. Microphthalmia-associated transcription factor (MITF), a target of miR-340, served as pivotal transcription factor involved in osteoclast differentiation. However, the role of miR-340 and MITF during osteoclast differentiation has not yet been clearly established. Tartrate-resistant acid phosphatase (TRAP) staining assay was performed to identify osteoclasts differentiated from bone marrow-derived macrophages (BMMs). Quantitative reverse transcription PCR (qRT-PCR) or Western blotting was undertaken to examine the mRNA or protein expression respectively. Luciferase reporter assay was performed to investigate the interaction between miR-340 and MITF. MITF was knocked down and miR-340 was overexpressed and transfected into BMMs to detect their effects on osteoclast differentiation. Firstly, qRT-PCR analysis showed that miR-340 was down-regulated during osteoclast differentiation stimulated by macrophage-colony stimulating factor (M-CSF) and receptor activator of nuclear factor (NF)-κB (RANK) ligand (RANKL). Besides, we found that overexpression of miRNA-340 inhibited osteoclast differentiation and suppressed both the mRNA and protein level of MITF. Finally, Western blot and qRT-PCR analysis revealed that silencing MITF inhibited TRAP, calcitonin receptor, V-ATPase d2, and cathepsin K. miR-340 suppresses osteoclast differentiation by inhibiting MITF. Our findings may provide promising therapeutic targets for osteoclast-associated diseases. |
format | Online Article Text |
id | pubmed-5518531 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-55185312017-10-17 miRNA-340 inhibits osteoclast differentiation via repression of MITF Zhao, Hongying Zhang, Jun Shao, Haiyu Liu, Jianwen Jin, Mengran Chen, Jinping Huang, Yazeng Biosci Rep Research Articles Many miRNAs play critical roles in modulating various biological processes of osteoclast differentiation and function. Microphthalmia-associated transcription factor (MITF), a target of miR-340, served as pivotal transcription factor involved in osteoclast differentiation. However, the role of miR-340 and MITF during osteoclast differentiation has not yet been clearly established. Tartrate-resistant acid phosphatase (TRAP) staining assay was performed to identify osteoclasts differentiated from bone marrow-derived macrophages (BMMs). Quantitative reverse transcription PCR (qRT-PCR) or Western blotting was undertaken to examine the mRNA or protein expression respectively. Luciferase reporter assay was performed to investigate the interaction between miR-340 and MITF. MITF was knocked down and miR-340 was overexpressed and transfected into BMMs to detect their effects on osteoclast differentiation. Firstly, qRT-PCR analysis showed that miR-340 was down-regulated during osteoclast differentiation stimulated by macrophage-colony stimulating factor (M-CSF) and receptor activator of nuclear factor (NF)-κB (RANK) ligand (RANKL). Besides, we found that overexpression of miRNA-340 inhibited osteoclast differentiation and suppressed both the mRNA and protein level of MITF. Finally, Western blot and qRT-PCR analysis revealed that silencing MITF inhibited TRAP, calcitonin receptor, V-ATPase d2, and cathepsin K. miR-340 suppresses osteoclast differentiation by inhibiting MITF. Our findings may provide promising therapeutic targets for osteoclast-associated diseases. Portland Press Ltd. 2017-07-20 /pmc/articles/PMC5518531/ /pubmed/28607030 http://dx.doi.org/10.1042/BSR20170302 Text en © 2017 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Zhao, Hongying Zhang, Jun Shao, Haiyu Liu, Jianwen Jin, Mengran Chen, Jinping Huang, Yazeng miRNA-340 inhibits osteoclast differentiation via repression of MITF |
title | miRNA-340 inhibits osteoclast differentiation via repression of MITF |
title_full | miRNA-340 inhibits osteoclast differentiation via repression of MITF |
title_fullStr | miRNA-340 inhibits osteoclast differentiation via repression of MITF |
title_full_unstemmed | miRNA-340 inhibits osteoclast differentiation via repression of MITF |
title_short | miRNA-340 inhibits osteoclast differentiation via repression of MITF |
title_sort | mirna-340 inhibits osteoclast differentiation via repression of mitf |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5518531/ https://www.ncbi.nlm.nih.gov/pubmed/28607030 http://dx.doi.org/10.1042/BSR20170302 |
work_keys_str_mv | AT zhaohongying mirna340inhibitsosteoclastdifferentiationviarepressionofmitf AT zhangjun mirna340inhibitsosteoclastdifferentiationviarepressionofmitf AT shaohaiyu mirna340inhibitsosteoclastdifferentiationviarepressionofmitf AT liujianwen mirna340inhibitsosteoclastdifferentiationviarepressionofmitf AT jinmengran mirna340inhibitsosteoclastdifferentiationviarepressionofmitf AT chenjinping mirna340inhibitsosteoclastdifferentiationviarepressionofmitf AT huangyazeng mirna340inhibitsosteoclastdifferentiationviarepressionofmitf |