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Sugary Endosperm is Modulated by Starch Branching Enzyme IIa in Rice (Oryza sativa L.)

BACKGROUND: Starch biosynthesis is one of the most important pathways that determine both grain quality and yield in rice (Oryza sativa L.). Sugary endosperm, sugary-1 (sug-1), is a mutant trait for starch biosynthesis. Rice plants carrying sug-1 produce grains that accumulate water-soluble carbohyd...

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Autores principales: Lee, Yunjoo, Choi, Min-Seon, Lee, Gileung, Jang, Su, Yoon, Mi-Ra, Kim, Backki, Piao, Rihua, Woo, Mi-Ok, Chin, Joong Hyoun, Koh, Hee-Jong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5519516/
https://www.ncbi.nlm.nih.gov/pubmed/28730411
http://dx.doi.org/10.1186/s12284-017-0172-3
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author Lee, Yunjoo
Choi, Min-Seon
Lee, Gileung
Jang, Su
Yoon, Mi-Ra
Kim, Backki
Piao, Rihua
Woo, Mi-Ok
Chin, Joong Hyoun
Koh, Hee-Jong
author_facet Lee, Yunjoo
Choi, Min-Seon
Lee, Gileung
Jang, Su
Yoon, Mi-Ra
Kim, Backki
Piao, Rihua
Woo, Mi-Ok
Chin, Joong Hyoun
Koh, Hee-Jong
author_sort Lee, Yunjoo
collection PubMed
description BACKGROUND: Starch biosynthesis is one of the most important pathways that determine both grain quality and yield in rice (Oryza sativa L.). Sugary endosperm, sugary-1 (sug-1), is a mutant trait for starch biosynthesis. Rice plants carrying sug-1 produce grains that accumulate water-soluble carbohydrates instead of starch, even after maturity. Although this trait enhances the diversity of grain quality, sugary endosperm rice has hardly been commercialized due to the severely wrinkled grains and subsequent problems in milling. This study was conducted to identify the genes responsible for the sug-h phenotype through a map-based cloning technology. RESULTS: We induced a mild sugary mutant, sugary-h (sug-h) through the chemical mutagenesis on the Korean japonica cultivar Hwacheong. Grains of the sug-h mutant were translucent and amber-colored, and the endosperm appeared less wrinkled than sug-1, whereas the soluble sugar content was fairly high. These characteristics confer greater marketability to the sug-h mutant. Genetic analyses indicated that the sug-h mutant phenotype was controlled by a complementary interaction of two recessive genes, Isoamylase1 (OsISA1), which was reported previously, and Starch branching enzyme IIa (OsBEIIa), which was newly identified in this study. Complementation tests indicated that OsBEIIa regulated the properties of sugary endosperm. CONCLUSIONS: Complementary interactions between the starch biosynthesis genes OsISA1 and OsBEIIa determine the mild sugary endosperm mutant, sugary-h, in rice. Our finding may facilitate the breeding of sugaryendosperm rice for commercial benefit. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12284-017-0172-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-55195162017-08-02 Sugary Endosperm is Modulated by Starch Branching Enzyme IIa in Rice (Oryza sativa L.) Lee, Yunjoo Choi, Min-Seon Lee, Gileung Jang, Su Yoon, Mi-Ra Kim, Backki Piao, Rihua Woo, Mi-Ok Chin, Joong Hyoun Koh, Hee-Jong Rice (N Y) Original Article BACKGROUND: Starch biosynthesis is one of the most important pathways that determine both grain quality and yield in rice (Oryza sativa L.). Sugary endosperm, sugary-1 (sug-1), is a mutant trait for starch biosynthesis. Rice plants carrying sug-1 produce grains that accumulate water-soluble carbohydrates instead of starch, even after maturity. Although this trait enhances the diversity of grain quality, sugary endosperm rice has hardly been commercialized due to the severely wrinkled grains and subsequent problems in milling. This study was conducted to identify the genes responsible for the sug-h phenotype through a map-based cloning technology. RESULTS: We induced a mild sugary mutant, sugary-h (sug-h) through the chemical mutagenesis on the Korean japonica cultivar Hwacheong. Grains of the sug-h mutant were translucent and amber-colored, and the endosperm appeared less wrinkled than sug-1, whereas the soluble sugar content was fairly high. These characteristics confer greater marketability to the sug-h mutant. Genetic analyses indicated that the sug-h mutant phenotype was controlled by a complementary interaction of two recessive genes, Isoamylase1 (OsISA1), which was reported previously, and Starch branching enzyme IIa (OsBEIIa), which was newly identified in this study. Complementation tests indicated that OsBEIIa regulated the properties of sugary endosperm. CONCLUSIONS: Complementary interactions between the starch biosynthesis genes OsISA1 and OsBEIIa determine the mild sugary endosperm mutant, sugary-h, in rice. Our finding may facilitate the breeding of sugaryendosperm rice for commercial benefit. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12284-017-0172-3) contains supplementary material, which is available to authorized users. Springer US 2017-07-20 /pmc/articles/PMC5519516/ /pubmed/28730411 http://dx.doi.org/10.1186/s12284-017-0172-3 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Lee, Yunjoo
Choi, Min-Seon
Lee, Gileung
Jang, Su
Yoon, Mi-Ra
Kim, Backki
Piao, Rihua
Woo, Mi-Ok
Chin, Joong Hyoun
Koh, Hee-Jong
Sugary Endosperm is Modulated by Starch Branching Enzyme IIa in Rice (Oryza sativa L.)
title Sugary Endosperm is Modulated by Starch Branching Enzyme IIa in Rice (Oryza sativa L.)
title_full Sugary Endosperm is Modulated by Starch Branching Enzyme IIa in Rice (Oryza sativa L.)
title_fullStr Sugary Endosperm is Modulated by Starch Branching Enzyme IIa in Rice (Oryza sativa L.)
title_full_unstemmed Sugary Endosperm is Modulated by Starch Branching Enzyme IIa in Rice (Oryza sativa L.)
title_short Sugary Endosperm is Modulated by Starch Branching Enzyme IIa in Rice (Oryza sativa L.)
title_sort sugary endosperm is modulated by starch branching enzyme iia in rice (oryza sativa l.)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5519516/
https://www.ncbi.nlm.nih.gov/pubmed/28730411
http://dx.doi.org/10.1186/s12284-017-0172-3
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