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AM-37 and ST-36 Are Small Molecule Bombesin Receptor Antagonists

While peptide antagonists for the gastrin-releasing peptide receptor (BB(2)R), neuromedin B receptor (BB(1)R), and bombesin (BB) receptor subtype-3 (BRS-3) exist, there is a need to develop non-peptide small molecule inhibitors for all three BBR. The BB agonist (BA)1 binds with high affinity to the...

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Autores principales: Moody, Terry W., Tashakkori, Nicole, Mantey, Samuel A., Moreno, Paola, Ramos-Alvarez, Irene, Leopoldo, Marcello, Jensen, Robert T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5519534/
https://www.ncbi.nlm.nih.gov/pubmed/28785244
http://dx.doi.org/10.3389/fendo.2017.00176
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author Moody, Terry W.
Tashakkori, Nicole
Mantey, Samuel A.
Moreno, Paola
Ramos-Alvarez, Irene
Leopoldo, Marcello
Jensen, Robert T.
author_facet Moody, Terry W.
Tashakkori, Nicole
Mantey, Samuel A.
Moreno, Paola
Ramos-Alvarez, Irene
Leopoldo, Marcello
Jensen, Robert T.
author_sort Moody, Terry W.
collection PubMed
description While peptide antagonists for the gastrin-releasing peptide receptor (BB(2)R), neuromedin B receptor (BB(1)R), and bombesin (BB) receptor subtype-3 (BRS-3) exist, there is a need to develop non-peptide small molecule inhibitors for all three BBR. The BB agonist (BA)1 binds with high affinity to the BB(1)R, BB(2)R, and BRS-3. In this communication, small molecule BBR antagonists were evaluated using human lung cancer cells. AM-37 and ST-36 inhibited binding to human BB(1)R, BB(2)R, and BRS-3 with similar affinity (Ki = 1.4–10.8 µM). AM-13 and AM-14 were approximately an order of magnitude less potent than AM-37 and ST-36. The ability of BA1 to elevate cytosolic Ca(2+) in human lung cancer cells transfected with BB(1)R, BB(2)R, and BRS-3 was antagonized by AM-37 and ST-36. BA1 increased tyrosine phosphorylation of the EGFR and ERK in lung cancer cells, which was blocked by AM-37 and ST-36. AM-37 and ST-36 reduced the growth of lung cancer cells that have BBR. The results indicate that AM-37 and ST-36 function as small molecule BB receptor antagonists.
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spelling pubmed-55195342017-08-07 AM-37 and ST-36 Are Small Molecule Bombesin Receptor Antagonists Moody, Terry W. Tashakkori, Nicole Mantey, Samuel A. Moreno, Paola Ramos-Alvarez, Irene Leopoldo, Marcello Jensen, Robert T. Front Endocrinol (Lausanne) Endocrinology While peptide antagonists for the gastrin-releasing peptide receptor (BB(2)R), neuromedin B receptor (BB(1)R), and bombesin (BB) receptor subtype-3 (BRS-3) exist, there is a need to develop non-peptide small molecule inhibitors for all three BBR. The BB agonist (BA)1 binds with high affinity to the BB(1)R, BB(2)R, and BRS-3. In this communication, small molecule BBR antagonists were evaluated using human lung cancer cells. AM-37 and ST-36 inhibited binding to human BB(1)R, BB(2)R, and BRS-3 with similar affinity (Ki = 1.4–10.8 µM). AM-13 and AM-14 were approximately an order of magnitude less potent than AM-37 and ST-36. The ability of BA1 to elevate cytosolic Ca(2+) in human lung cancer cells transfected with BB(1)R, BB(2)R, and BRS-3 was antagonized by AM-37 and ST-36. BA1 increased tyrosine phosphorylation of the EGFR and ERK in lung cancer cells, which was blocked by AM-37 and ST-36. AM-37 and ST-36 reduced the growth of lung cancer cells that have BBR. The results indicate that AM-37 and ST-36 function as small molecule BB receptor antagonists. Frontiers Media S.A. 2017-07-21 /pmc/articles/PMC5519534/ /pubmed/28785244 http://dx.doi.org/10.3389/fendo.2017.00176 Text en Copyright © 2017 Moody, Tashakkori, Mantey, Moreno, Ramos-Alvarez, Leopoldo and Jensen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Moody, Terry W.
Tashakkori, Nicole
Mantey, Samuel A.
Moreno, Paola
Ramos-Alvarez, Irene
Leopoldo, Marcello
Jensen, Robert T.
AM-37 and ST-36 Are Small Molecule Bombesin Receptor Antagonists
title AM-37 and ST-36 Are Small Molecule Bombesin Receptor Antagonists
title_full AM-37 and ST-36 Are Small Molecule Bombesin Receptor Antagonists
title_fullStr AM-37 and ST-36 Are Small Molecule Bombesin Receptor Antagonists
title_full_unstemmed AM-37 and ST-36 Are Small Molecule Bombesin Receptor Antagonists
title_short AM-37 and ST-36 Are Small Molecule Bombesin Receptor Antagonists
title_sort am-37 and st-36 are small molecule bombesin receptor antagonists
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5519534/
https://www.ncbi.nlm.nih.gov/pubmed/28785244
http://dx.doi.org/10.3389/fendo.2017.00176
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