Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles

Microbial single-cell genomics can be used to provide insights into the metabolic potential, interactions, and evolution of uncultured microorganisms. Here we present WGA-X, a method based on multiple displacement amplification of DNA that utilizes a thermostable mutant of the phi29 polymerase. WGA-...

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Detalles Bibliográficos
Autores principales: Stepanauskas, Ramunas, Fergusson, Elizabeth A., Brown, Joseph, Poulton, Nicole J., Tupper, Ben, Labonté, Jessica M., Becraft, Eric D., Brown, Julia M., Pachiadaki, Maria G., Povilaitis, Tadas, Thompson, Brian P., Mascena, Corianna J., Bellows, Wendy K., Lubys, Arvydas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5519541/
https://www.ncbi.nlm.nih.gov/pubmed/28729688
http://dx.doi.org/10.1038/s41467-017-00128-z

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