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The thrombopoietin/MPL axis is activated in the Gata1(low) mouse model of myelofibrosis and is associated with a defective RPS14 signature

Myelofibrosis (MF) is characterized by hyperactivation of thrombopoietin (TPO) signaling, which induces a RPS14 deficiency that de-regulates GATA1 in megakaryocytes by hampering its mRNA translation. As mice carrying the hypomorphic Gata1(low) mutation, which reduces the levels of Gata1 mRNA in mega...

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Autores principales: Zingariello, M, Sancillo, L, Martelli, F, Ciaffoni, F, Marra, M, Varricchio, L, Rana, R A, Zhao, C, Crispino, J D, Migliaccio, A R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5520398/
https://www.ncbi.nlm.nih.gov/pubmed/28622305
http://dx.doi.org/10.1038/bcj.2017.51
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author Zingariello, M
Sancillo, L
Martelli, F
Ciaffoni, F
Marra, M
Varricchio, L
Rana, R A
Zhao, C
Crispino, J D
Migliaccio, A R
author_facet Zingariello, M
Sancillo, L
Martelli, F
Ciaffoni, F
Marra, M
Varricchio, L
Rana, R A
Zhao, C
Crispino, J D
Migliaccio, A R
author_sort Zingariello, M
collection PubMed
description Myelofibrosis (MF) is characterized by hyperactivation of thrombopoietin (TPO) signaling, which induces a RPS14 deficiency that de-regulates GATA1 in megakaryocytes by hampering its mRNA translation. As mice carrying the hypomorphic Gata1(low) mutation, which reduces the levels of Gata1 mRNA in megakaryocytes, develop MF, we investigated whether the TPO axis is hyperactive in this model. Gata1(low) mice contained two times more Tpo mRNA in liver and TPO in plasma than wild-type littermates. Furthermore, Gata1(low) LSKs expressed levels of Mpl mRNA (five times greater than normal) and protein (two times lower than normal) similar to those expressed by LSKs from TPO-treated wild-type mice. Gata1(low) marrow and spleen contained more JAK2/STAT5 than wild-type tissues, an indication that these organs were reach of TPO-responsive cells. Moreover, treatment of Gata1(low) mice with the JAK inhibitor ruxolitinib reduced their splenomegaly. Also in Gata1(low) mice activation of the TPO/MPL axis was associated with a RSP14 deficiency and a discordant microarray ribosome signature (reduced RPS24, RPS26 and SBDS expression). Finally, electron microscopy revealed that Gata1(low) megakaryocytes contained poorly developed endoplasmic reticulum with rare polysomes. In summary, Gata1(low) mice are a bona fide model of MF, which recapitulates the hyperactivation of the TPO/MPL/JAK2 axis observed in megakaryocytes from myelofibrotic patients.
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spelling pubmed-55203982017-07-26 The thrombopoietin/MPL axis is activated in the Gata1(low) mouse model of myelofibrosis and is associated with a defective RPS14 signature Zingariello, M Sancillo, L Martelli, F Ciaffoni, F Marra, M Varricchio, L Rana, R A Zhao, C Crispino, J D Migliaccio, A R Blood Cancer J Original Article Myelofibrosis (MF) is characterized by hyperactivation of thrombopoietin (TPO) signaling, which induces a RPS14 deficiency that de-regulates GATA1 in megakaryocytes by hampering its mRNA translation. As mice carrying the hypomorphic Gata1(low) mutation, which reduces the levels of Gata1 mRNA in megakaryocytes, develop MF, we investigated whether the TPO axis is hyperactive in this model. Gata1(low) mice contained two times more Tpo mRNA in liver and TPO in plasma than wild-type littermates. Furthermore, Gata1(low) LSKs expressed levels of Mpl mRNA (five times greater than normal) and protein (two times lower than normal) similar to those expressed by LSKs from TPO-treated wild-type mice. Gata1(low) marrow and spleen contained more JAK2/STAT5 than wild-type tissues, an indication that these organs were reach of TPO-responsive cells. Moreover, treatment of Gata1(low) mice with the JAK inhibitor ruxolitinib reduced their splenomegaly. Also in Gata1(low) mice activation of the TPO/MPL axis was associated with a RSP14 deficiency and a discordant microarray ribosome signature (reduced RPS24, RPS26 and SBDS expression). Finally, electron microscopy revealed that Gata1(low) megakaryocytes contained poorly developed endoplasmic reticulum with rare polysomes. In summary, Gata1(low) mice are a bona fide model of MF, which recapitulates the hyperactivation of the TPO/MPL/JAK2 axis observed in megakaryocytes from myelofibrotic patients. Nature Publishing Group 2017-06 2017-06-16 /pmc/articles/PMC5520398/ /pubmed/28622305 http://dx.doi.org/10.1038/bcj.2017.51 Text en Copyright © 2017 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Original Article
Zingariello, M
Sancillo, L
Martelli, F
Ciaffoni, F
Marra, M
Varricchio, L
Rana, R A
Zhao, C
Crispino, J D
Migliaccio, A R
The thrombopoietin/MPL axis is activated in the Gata1(low) mouse model of myelofibrosis and is associated with a defective RPS14 signature
title The thrombopoietin/MPL axis is activated in the Gata1(low) mouse model of myelofibrosis and is associated with a defective RPS14 signature
title_full The thrombopoietin/MPL axis is activated in the Gata1(low) mouse model of myelofibrosis and is associated with a defective RPS14 signature
title_fullStr The thrombopoietin/MPL axis is activated in the Gata1(low) mouse model of myelofibrosis and is associated with a defective RPS14 signature
title_full_unstemmed The thrombopoietin/MPL axis is activated in the Gata1(low) mouse model of myelofibrosis and is associated with a defective RPS14 signature
title_short The thrombopoietin/MPL axis is activated in the Gata1(low) mouse model of myelofibrosis and is associated with a defective RPS14 signature
title_sort thrombopoietin/mpl axis is activated in the gata1(low) mouse model of myelofibrosis and is associated with a defective rps14 signature
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5520398/
https://www.ncbi.nlm.nih.gov/pubmed/28622305
http://dx.doi.org/10.1038/bcj.2017.51
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