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In Vivo Osteogenic Differentiation of Human Embryoid Bodies in an Injectable in Situ-Forming Hydrogel

In this study, we examined the in vivo osteogenic differentiation of human embryoid bodies (hEBs) by using an injectable in situ-forming hydrogel. A solution containing MPEG-b-(polycaprolactone-ran-polylactide) (MCL) and hEBs was easily prepared at room temperature. The MCL solution with hEBs and os...

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Autores principales: Kim, Da Yeon, Kim, Yoon Young, Lee, Hai Bang, Moon, Shin Yong, Ku, Seung-Yup, Kim, Moon Suk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5521290/
https://www.ncbi.nlm.nih.gov/pubmed/28811417
http://dx.doi.org/10.3390/ma6072978
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author Kim, Da Yeon
Kim, Yoon Young
Lee, Hai Bang
Moon, Shin Yong
Ku, Seung-Yup
Kim, Moon Suk
author_facet Kim, Da Yeon
Kim, Yoon Young
Lee, Hai Bang
Moon, Shin Yong
Ku, Seung-Yup
Kim, Moon Suk
author_sort Kim, Da Yeon
collection PubMed
description In this study, we examined the in vivo osteogenic differentiation of human embryoid bodies (hEBs) by using an injectable in situ-forming hydrogel. A solution containing MPEG-b-(polycaprolactone-ran-polylactide) (MCL) and hEBs was easily prepared at room temperature. The MCL solution with hEBs and osteogenic factors was injected into nude mice and developed into in situ-forming hydrogels at the injection sites; these hydrogels maintained their shape even after 12 weeks in vivo, thereby indicating that the in situ-forming MCL hydrogel was a suitable scaffold for hEBs. The in vivo osteogenic differentiation was observed only in the in situ gel-forming MCL hydrogel in the presence of hEBs and osteogenic factors. In conclusion, this preliminary study suggests that hEBs and osteogenic factors embedded in an in situ-forming MCL hydrogel may provide numerous benefits as a noninvasive alternative for allogeneic tissue engineering applications.
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spelling pubmed-55212902017-07-28 In Vivo Osteogenic Differentiation of Human Embryoid Bodies in an Injectable in Situ-Forming Hydrogel Kim, Da Yeon Kim, Yoon Young Lee, Hai Bang Moon, Shin Yong Ku, Seung-Yup Kim, Moon Suk Materials (Basel) Article In this study, we examined the in vivo osteogenic differentiation of human embryoid bodies (hEBs) by using an injectable in situ-forming hydrogel. A solution containing MPEG-b-(polycaprolactone-ran-polylactide) (MCL) and hEBs was easily prepared at room temperature. The MCL solution with hEBs and osteogenic factors was injected into nude mice and developed into in situ-forming hydrogels at the injection sites; these hydrogels maintained their shape even after 12 weeks in vivo, thereby indicating that the in situ-forming MCL hydrogel was a suitable scaffold for hEBs. The in vivo osteogenic differentiation was observed only in the in situ gel-forming MCL hydrogel in the presence of hEBs and osteogenic factors. In conclusion, this preliminary study suggests that hEBs and osteogenic factors embedded in an in situ-forming MCL hydrogel may provide numerous benefits as a noninvasive alternative for allogeneic tissue engineering applications. MDPI 2013-07-17 /pmc/articles/PMC5521290/ /pubmed/28811417 http://dx.doi.org/10.3390/ma6072978 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Kim, Da Yeon
Kim, Yoon Young
Lee, Hai Bang
Moon, Shin Yong
Ku, Seung-Yup
Kim, Moon Suk
In Vivo Osteogenic Differentiation of Human Embryoid Bodies in an Injectable in Situ-Forming Hydrogel
title In Vivo Osteogenic Differentiation of Human Embryoid Bodies in an Injectable in Situ-Forming Hydrogel
title_full In Vivo Osteogenic Differentiation of Human Embryoid Bodies in an Injectable in Situ-Forming Hydrogel
title_fullStr In Vivo Osteogenic Differentiation of Human Embryoid Bodies in an Injectable in Situ-Forming Hydrogel
title_full_unstemmed In Vivo Osteogenic Differentiation of Human Embryoid Bodies in an Injectable in Situ-Forming Hydrogel
title_short In Vivo Osteogenic Differentiation of Human Embryoid Bodies in an Injectable in Situ-Forming Hydrogel
title_sort in vivo osteogenic differentiation of human embryoid bodies in an injectable in situ-forming hydrogel
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5521290/
https://www.ncbi.nlm.nih.gov/pubmed/28811417
http://dx.doi.org/10.3390/ma6072978
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