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Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae)

Dengue represents a serious threat to human health, with billions of people living at risk of the disease. Wolbachia pipientis is a bacterial endosymbiont common to many insect species. Wolbachia transinfections in mosquito disease vectors have great value for disease control given the bacterium’s a...

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Autores principales: Silva, Jéssica Barreto Lopes, Magalhães Alves, Debora, Bottino-Rojas, Vanessa, Pereira, Thiago Nunes, Sorgine, Marcos Henrique Ferreira, Caragata, Eric Pearce, Moreira, Luciano Andrade
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5521830/
https://www.ncbi.nlm.nih.gov/pubmed/28732048
http://dx.doi.org/10.1371/journal.pone.0181678
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author Silva, Jéssica Barreto Lopes
Magalhães Alves, Debora
Bottino-Rojas, Vanessa
Pereira, Thiago Nunes
Sorgine, Marcos Henrique Ferreira
Caragata, Eric Pearce
Moreira, Luciano Andrade
author_facet Silva, Jéssica Barreto Lopes
Magalhães Alves, Debora
Bottino-Rojas, Vanessa
Pereira, Thiago Nunes
Sorgine, Marcos Henrique Ferreira
Caragata, Eric Pearce
Moreira, Luciano Andrade
author_sort Silva, Jéssica Barreto Lopes
collection PubMed
description Dengue represents a serious threat to human health, with billions of people living at risk of the disease. Wolbachia pipientis is a bacterial endosymbiont common to many insect species. Wolbachia transinfections in mosquito disease vectors have great value for disease control given the bacterium’s ability to spread into wild mosquito populations, and to interfere with infections of pathogens, such as dengue virus. Aedes fluviatilis is a mosquito with a widespread distribution in Latin America, but its status as a dengue vector has not been clarified. Ae. fluviatilis is also naturally infected by the wFlu Wolbachia strain, which has been demonstrated to enhance infection with the avian malarial parasite Plasmodium gallinaceum. We performed experimental infections of Ae. fluviatilis with DENV-2 and DENV-3 isolates from Brazil via injection or oral feeding to provide insight into its competence for the virus. We also examined the effect of the native Wolbachia infection on the virus using a mosquito line where the wFlu infection had been cleared by antibiotic treatment. Through RT-qPCR, we observed that Ae. fluviatilis could become infected with both viruses via either method of infection, although at a lower rate than Aedes aegypti, the primary dengue vector. We then detected DENV-2 and DENV-3 in the saliva of injected mosquitoes, and observed that injection of DENV-3-infected saliva produced subsequent infections in naïve Ae. aegypti. However, across our data we observed no difference in prevalence of infection and viral load between Wolbachia-infected and -uninfected mosquitoes, suggesting that there is no effect of wFlu on dengue virus. Our results highlight that Ae. fluviatilis could potentially serve as a dengue vector under the right circumstances, although further testing is required to determine if this occurs in the field.
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spelling pubmed-55218302017-08-07 Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae) Silva, Jéssica Barreto Lopes Magalhães Alves, Debora Bottino-Rojas, Vanessa Pereira, Thiago Nunes Sorgine, Marcos Henrique Ferreira Caragata, Eric Pearce Moreira, Luciano Andrade PLoS One Research Article Dengue represents a serious threat to human health, with billions of people living at risk of the disease. Wolbachia pipientis is a bacterial endosymbiont common to many insect species. Wolbachia transinfections in mosquito disease vectors have great value for disease control given the bacterium’s ability to spread into wild mosquito populations, and to interfere with infections of pathogens, such as dengue virus. Aedes fluviatilis is a mosquito with a widespread distribution in Latin America, but its status as a dengue vector has not been clarified. Ae. fluviatilis is also naturally infected by the wFlu Wolbachia strain, which has been demonstrated to enhance infection with the avian malarial parasite Plasmodium gallinaceum. We performed experimental infections of Ae. fluviatilis with DENV-2 and DENV-3 isolates from Brazil via injection or oral feeding to provide insight into its competence for the virus. We also examined the effect of the native Wolbachia infection on the virus using a mosquito line where the wFlu infection had been cleared by antibiotic treatment. Through RT-qPCR, we observed that Ae. fluviatilis could become infected with both viruses via either method of infection, although at a lower rate than Aedes aegypti, the primary dengue vector. We then detected DENV-2 and DENV-3 in the saliva of injected mosquitoes, and observed that injection of DENV-3-infected saliva produced subsequent infections in naïve Ae. aegypti. However, across our data we observed no difference in prevalence of infection and viral load between Wolbachia-infected and -uninfected mosquitoes, suggesting that there is no effect of wFlu on dengue virus. Our results highlight that Ae. fluviatilis could potentially serve as a dengue vector under the right circumstances, although further testing is required to determine if this occurs in the field. Public Library of Science 2017-07-21 /pmc/articles/PMC5521830/ /pubmed/28732048 http://dx.doi.org/10.1371/journal.pone.0181678 Text en © 2017 Silva et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Silva, Jéssica Barreto Lopes
Magalhães Alves, Debora
Bottino-Rojas, Vanessa
Pereira, Thiago Nunes
Sorgine, Marcos Henrique Ferreira
Caragata, Eric Pearce
Moreira, Luciano Andrade
Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae)
title Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae)
title_full Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae)
title_fullStr Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae)
title_full_unstemmed Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae)
title_short Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae)
title_sort wolbachia and dengue virus infection in the mosquito aedes fluviatilis (diptera: culicidae)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5521830/
https://www.ncbi.nlm.nih.gov/pubmed/28732048
http://dx.doi.org/10.1371/journal.pone.0181678
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