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A subset of microRNAs defining the side population of a human malignant mesothelioma cell line

This study was performed to investigate the global expression profile of microRNAs in distinct subpopulations of a human malignant mesothelioma cell line. Total RNAs were isolated from the sorted side population and non-side population of MS1. The RNAs were subjected to analysis using Affymetrix Gen...

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Autores principales: Kim, Myung-Chul, Kim, Na-Yon, Seo, Yu-Ri, Kim, Yongbaek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522110/
https://www.ncbi.nlm.nih.gov/pubmed/28467812
http://dx.doi.org/10.18632/oncotarget.17086
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author Kim, Myung-Chul
Kim, Na-Yon
Seo, Yu-Ri
Kim, Yongbaek
author_facet Kim, Myung-Chul
Kim, Na-Yon
Seo, Yu-Ri
Kim, Yongbaek
author_sort Kim, Myung-Chul
collection PubMed
description This study was performed to investigate the global expression profile of microRNAs in distinct subpopulations of a human malignant mesothelioma cell line. Total RNAs were isolated from the sorted side population and non-side population of MS1. The RNAs were subjected to analysis using Affymetrix GeneChip microRNA Arrays. After data extraction and normalization, a subset of microRNAs defining cell subpopulations was identified using bioinformatics softwares. Based on the criteria of 2-fold difference and the p-value of < 0.05, a total of 95 microRNAs were differentially expressed in the side population compared to the non-side population. Functional ontology revealed that target genes of the miRNAs were categorized into various gene ontology terms, such as stem cell maintenance, cell proliferation, programmed cell death, cell migration, and cellular response to stress. The Kyoto Encyclopedia of Genes and Genomes analysis showed that ErbB-2 receptor tyrosine kinases signaling pathway was the most represented. Integrated analysis of MiRTarBase and RNA-seq identified 12 target genes of microRNAs defining side population, including DDIT4 and ROCK2. The present study indicates that a distinct set of microRNAs may be critically involved in the generation and maintenance of heterogeneous subpopulations of cancer cells. They could be a plausible target for the eradication of more aggressive cancer cell subpopulations.
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spelling pubmed-55221102017-08-08 A subset of microRNAs defining the side population of a human malignant mesothelioma cell line Kim, Myung-Chul Kim, Na-Yon Seo, Yu-Ri Kim, Yongbaek Oncotarget Research Paper This study was performed to investigate the global expression profile of microRNAs in distinct subpopulations of a human malignant mesothelioma cell line. Total RNAs were isolated from the sorted side population and non-side population of MS1. The RNAs were subjected to analysis using Affymetrix GeneChip microRNA Arrays. After data extraction and normalization, a subset of microRNAs defining cell subpopulations was identified using bioinformatics softwares. Based on the criteria of 2-fold difference and the p-value of < 0.05, a total of 95 microRNAs were differentially expressed in the side population compared to the non-side population. Functional ontology revealed that target genes of the miRNAs were categorized into various gene ontology terms, such as stem cell maintenance, cell proliferation, programmed cell death, cell migration, and cellular response to stress. The Kyoto Encyclopedia of Genes and Genomes analysis showed that ErbB-2 receptor tyrosine kinases signaling pathway was the most represented. Integrated analysis of MiRTarBase and RNA-seq identified 12 target genes of microRNAs defining side population, including DDIT4 and ROCK2. The present study indicates that a distinct set of microRNAs may be critically involved in the generation and maintenance of heterogeneous subpopulations of cancer cells. They could be a plausible target for the eradication of more aggressive cancer cell subpopulations. Impact Journals LLC 2017-04-13 /pmc/articles/PMC5522110/ /pubmed/28467812 http://dx.doi.org/10.18632/oncotarget.17086 Text en Copyright: © 2017 Kim et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Kim, Myung-Chul
Kim, Na-Yon
Seo, Yu-Ri
Kim, Yongbaek
A subset of microRNAs defining the side population of a human malignant mesothelioma cell line
title A subset of microRNAs defining the side population of a human malignant mesothelioma cell line
title_full A subset of microRNAs defining the side population of a human malignant mesothelioma cell line
title_fullStr A subset of microRNAs defining the side population of a human malignant mesothelioma cell line
title_full_unstemmed A subset of microRNAs defining the side population of a human malignant mesothelioma cell line
title_short A subset of microRNAs defining the side population of a human malignant mesothelioma cell line
title_sort subset of micrornas defining the side population of a human malignant mesothelioma cell line
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522110/
https://www.ncbi.nlm.nih.gov/pubmed/28467812
http://dx.doi.org/10.18632/oncotarget.17086
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