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A subset of microRNAs defining the side population of a human malignant mesothelioma cell line
This study was performed to investigate the global expression profile of microRNAs in distinct subpopulations of a human malignant mesothelioma cell line. Total RNAs were isolated from the sorted side population and non-side population of MS1. The RNAs were subjected to analysis using Affymetrix Gen...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522110/ https://www.ncbi.nlm.nih.gov/pubmed/28467812 http://dx.doi.org/10.18632/oncotarget.17086 |
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author | Kim, Myung-Chul Kim, Na-Yon Seo, Yu-Ri Kim, Yongbaek |
author_facet | Kim, Myung-Chul Kim, Na-Yon Seo, Yu-Ri Kim, Yongbaek |
author_sort | Kim, Myung-Chul |
collection | PubMed |
description | This study was performed to investigate the global expression profile of microRNAs in distinct subpopulations of a human malignant mesothelioma cell line. Total RNAs were isolated from the sorted side population and non-side population of MS1. The RNAs were subjected to analysis using Affymetrix GeneChip microRNA Arrays. After data extraction and normalization, a subset of microRNAs defining cell subpopulations was identified using bioinformatics softwares. Based on the criteria of 2-fold difference and the p-value of < 0.05, a total of 95 microRNAs were differentially expressed in the side population compared to the non-side population. Functional ontology revealed that target genes of the miRNAs were categorized into various gene ontology terms, such as stem cell maintenance, cell proliferation, programmed cell death, cell migration, and cellular response to stress. The Kyoto Encyclopedia of Genes and Genomes analysis showed that ErbB-2 receptor tyrosine kinases signaling pathway was the most represented. Integrated analysis of MiRTarBase and RNA-seq identified 12 target genes of microRNAs defining side population, including DDIT4 and ROCK2. The present study indicates that a distinct set of microRNAs may be critically involved in the generation and maintenance of heterogeneous subpopulations of cancer cells. They could be a plausible target for the eradication of more aggressive cancer cell subpopulations. |
format | Online Article Text |
id | pubmed-5522110 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-55221102017-08-08 A subset of microRNAs defining the side population of a human malignant mesothelioma cell line Kim, Myung-Chul Kim, Na-Yon Seo, Yu-Ri Kim, Yongbaek Oncotarget Research Paper This study was performed to investigate the global expression profile of microRNAs in distinct subpopulations of a human malignant mesothelioma cell line. Total RNAs were isolated from the sorted side population and non-side population of MS1. The RNAs were subjected to analysis using Affymetrix GeneChip microRNA Arrays. After data extraction and normalization, a subset of microRNAs defining cell subpopulations was identified using bioinformatics softwares. Based on the criteria of 2-fold difference and the p-value of < 0.05, a total of 95 microRNAs were differentially expressed in the side population compared to the non-side population. Functional ontology revealed that target genes of the miRNAs were categorized into various gene ontology terms, such as stem cell maintenance, cell proliferation, programmed cell death, cell migration, and cellular response to stress. The Kyoto Encyclopedia of Genes and Genomes analysis showed that ErbB-2 receptor tyrosine kinases signaling pathway was the most represented. Integrated analysis of MiRTarBase and RNA-seq identified 12 target genes of microRNAs defining side population, including DDIT4 and ROCK2. The present study indicates that a distinct set of microRNAs may be critically involved in the generation and maintenance of heterogeneous subpopulations of cancer cells. They could be a plausible target for the eradication of more aggressive cancer cell subpopulations. Impact Journals LLC 2017-04-13 /pmc/articles/PMC5522110/ /pubmed/28467812 http://dx.doi.org/10.18632/oncotarget.17086 Text en Copyright: © 2017 Kim et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Research Paper Kim, Myung-Chul Kim, Na-Yon Seo, Yu-Ri Kim, Yongbaek A subset of microRNAs defining the side population of a human malignant mesothelioma cell line |
title | A subset of microRNAs defining the side population of a human malignant mesothelioma cell line |
title_full | A subset of microRNAs defining the side population of a human malignant mesothelioma cell line |
title_fullStr | A subset of microRNAs defining the side population of a human malignant mesothelioma cell line |
title_full_unstemmed | A subset of microRNAs defining the side population of a human malignant mesothelioma cell line |
title_short | A subset of microRNAs defining the side population of a human malignant mesothelioma cell line |
title_sort | subset of micrornas defining the side population of a human malignant mesothelioma cell line |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522110/ https://www.ncbi.nlm.nih.gov/pubmed/28467812 http://dx.doi.org/10.18632/oncotarget.17086 |
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