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Comparative analyses of long non-coding RNA in lean and obese pigs
OBJECTIVES: Current studies have revealed that long non-coding RNA plays a crucial role in fat metabolism. However, the difference of lncRNA between lean (Duroc) and obese (Luchuan) pig remain undefined. Here, we investigated the expressional profile of lncRNA in these two pigs and discussed the rel...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522191/ https://www.ncbi.nlm.nih.gov/pubmed/28589911 http://dx.doi.org/10.18632/oncotarget.18269 |
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author | Yu, Lin Tai, Lina Zhang, Lifang Chu, Yi Li, Yixing Zhou, Lei |
author_facet | Yu, Lin Tai, Lina Zhang, Lifang Chu, Yi Li, Yixing Zhou, Lei |
author_sort | Yu, Lin |
collection | PubMed |
description | OBJECTIVES: Current studies have revealed that long non-coding RNA plays a crucial role in fat metabolism. However, the difference of lncRNA between lean (Duroc) and obese (Luchuan) pig remain undefined. Here, we investigated the expressional profile of lncRNA in these two pigs and discussed the relationship between lncRNA and fat deposition. MATERIALS AND METHODS: The Chinese Luchuan pig has a dramatic differences in backfat thickness as compared with Duroc pig. In this study, 4868 lncRNA transcripts (including 3235 novel transcripts) were identified. We determined that patterns of differently expressed lncRNAs and mRNAs are strongly tissue-specific. The differentially expressed lncRNAs in adipose tissue have 794 potential target genes, which are involved in adipocytokine signaling pathways, the PI3k-Akt signaling pathway, and calcium signaling pathways. In addition, differentially expressed lncRNAs were located to 13 adipose-related quantitative trait loci which include 65 QTL_ID. Subsequently, lncRNA and mRNA in the same QTL_ID were analyzed and their co-expression in two QTL_ID were confirmed by qPCR. CONCLUSIONS: Our study provides an insight into mechanism behind the fat metabolic differences between the two breeds and lays an important groundwork for further research regarding the regulatory role of lncRNA in obesity development. |
format | Online Article Text |
id | pubmed-5522191 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-55221912017-08-21 Comparative analyses of long non-coding RNA in lean and obese pigs Yu, Lin Tai, Lina Zhang, Lifang Chu, Yi Li, Yixing Zhou, Lei Oncotarget Research Paper OBJECTIVES: Current studies have revealed that long non-coding RNA plays a crucial role in fat metabolism. However, the difference of lncRNA between lean (Duroc) and obese (Luchuan) pig remain undefined. Here, we investigated the expressional profile of lncRNA in these two pigs and discussed the relationship between lncRNA and fat deposition. MATERIALS AND METHODS: The Chinese Luchuan pig has a dramatic differences in backfat thickness as compared with Duroc pig. In this study, 4868 lncRNA transcripts (including 3235 novel transcripts) were identified. We determined that patterns of differently expressed lncRNAs and mRNAs are strongly tissue-specific. The differentially expressed lncRNAs in adipose tissue have 794 potential target genes, which are involved in adipocytokine signaling pathways, the PI3k-Akt signaling pathway, and calcium signaling pathways. In addition, differentially expressed lncRNAs were located to 13 adipose-related quantitative trait loci which include 65 QTL_ID. Subsequently, lncRNA and mRNA in the same QTL_ID were analyzed and their co-expression in two QTL_ID were confirmed by qPCR. CONCLUSIONS: Our study provides an insight into mechanism behind the fat metabolic differences between the two breeds and lays an important groundwork for further research regarding the regulatory role of lncRNA in obesity development. Impact Journals LLC 2017-05-26 /pmc/articles/PMC5522191/ /pubmed/28589911 http://dx.doi.org/10.18632/oncotarget.18269 Text en Copyright: © 2017 Yu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Yu, Lin Tai, Lina Zhang, Lifang Chu, Yi Li, Yixing Zhou, Lei Comparative analyses of long non-coding RNA in lean and obese pigs |
title | Comparative analyses of long non-coding RNA in lean and obese pigs |
title_full | Comparative analyses of long non-coding RNA in lean and obese pigs |
title_fullStr | Comparative analyses of long non-coding RNA in lean and obese pigs |
title_full_unstemmed | Comparative analyses of long non-coding RNA in lean and obese pigs |
title_short | Comparative analyses of long non-coding RNA in lean and obese pigs |
title_sort | comparative analyses of long non-coding rna in lean and obese pigs |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522191/ https://www.ncbi.nlm.nih.gov/pubmed/28589911 http://dx.doi.org/10.18632/oncotarget.18269 |
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