Cargando…

Comparative analyses of long non-coding RNA in lean and obese pigs

OBJECTIVES: Current studies have revealed that long non-coding RNA plays a crucial role in fat metabolism. However, the difference of lncRNA between lean (Duroc) and obese (Luchuan) pig remain undefined. Here, we investigated the expressional profile of lncRNA in these two pigs and discussed the rel...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Lin, Tai, Lina, Zhang, Lifang, Chu, Yi, Li, Yixing, Zhou, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522191/
https://www.ncbi.nlm.nih.gov/pubmed/28589911
http://dx.doi.org/10.18632/oncotarget.18269
_version_ 1783252118861250560
author Yu, Lin
Tai, Lina
Zhang, Lifang
Chu, Yi
Li, Yixing
Zhou, Lei
author_facet Yu, Lin
Tai, Lina
Zhang, Lifang
Chu, Yi
Li, Yixing
Zhou, Lei
author_sort Yu, Lin
collection PubMed
description OBJECTIVES: Current studies have revealed that long non-coding RNA plays a crucial role in fat metabolism. However, the difference of lncRNA between lean (Duroc) and obese (Luchuan) pig remain undefined. Here, we investigated the expressional profile of lncRNA in these two pigs and discussed the relationship between lncRNA and fat deposition. MATERIALS AND METHODS: The Chinese Luchuan pig has a dramatic differences in backfat thickness as compared with Duroc pig. In this study, 4868 lncRNA transcripts (including 3235 novel transcripts) were identified. We determined that patterns of differently expressed lncRNAs and mRNAs are strongly tissue-specific. The differentially expressed lncRNAs in adipose tissue have 794 potential target genes, which are involved in adipocytokine signaling pathways, the PI3k-Akt signaling pathway, and calcium signaling pathways. In addition, differentially expressed lncRNAs were located to 13 adipose-related quantitative trait loci which include 65 QTL_ID. Subsequently, lncRNA and mRNA in the same QTL_ID were analyzed and their co-expression in two QTL_ID were confirmed by qPCR. CONCLUSIONS: Our study provides an insight into mechanism behind the fat metabolic differences between the two breeds and lays an important groundwork for further research regarding the regulatory role of lncRNA in obesity development.
format Online
Article
Text
id pubmed-5522191
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-55221912017-08-21 Comparative analyses of long non-coding RNA in lean and obese pigs Yu, Lin Tai, Lina Zhang, Lifang Chu, Yi Li, Yixing Zhou, Lei Oncotarget Research Paper OBJECTIVES: Current studies have revealed that long non-coding RNA plays a crucial role in fat metabolism. However, the difference of lncRNA between lean (Duroc) and obese (Luchuan) pig remain undefined. Here, we investigated the expressional profile of lncRNA in these two pigs and discussed the relationship between lncRNA and fat deposition. MATERIALS AND METHODS: The Chinese Luchuan pig has a dramatic differences in backfat thickness as compared with Duroc pig. In this study, 4868 lncRNA transcripts (including 3235 novel transcripts) were identified. We determined that patterns of differently expressed lncRNAs and mRNAs are strongly tissue-specific. The differentially expressed lncRNAs in adipose tissue have 794 potential target genes, which are involved in adipocytokine signaling pathways, the PI3k-Akt signaling pathway, and calcium signaling pathways. In addition, differentially expressed lncRNAs were located to 13 adipose-related quantitative trait loci which include 65 QTL_ID. Subsequently, lncRNA and mRNA in the same QTL_ID were analyzed and their co-expression in two QTL_ID were confirmed by qPCR. CONCLUSIONS: Our study provides an insight into mechanism behind the fat metabolic differences between the two breeds and lays an important groundwork for further research regarding the regulatory role of lncRNA in obesity development. Impact Journals LLC 2017-05-26 /pmc/articles/PMC5522191/ /pubmed/28589911 http://dx.doi.org/10.18632/oncotarget.18269 Text en Copyright: © 2017 Yu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Yu, Lin
Tai, Lina
Zhang, Lifang
Chu, Yi
Li, Yixing
Zhou, Lei
Comparative analyses of long non-coding RNA in lean and obese pigs
title Comparative analyses of long non-coding RNA in lean and obese pigs
title_full Comparative analyses of long non-coding RNA in lean and obese pigs
title_fullStr Comparative analyses of long non-coding RNA in lean and obese pigs
title_full_unstemmed Comparative analyses of long non-coding RNA in lean and obese pigs
title_short Comparative analyses of long non-coding RNA in lean and obese pigs
title_sort comparative analyses of long non-coding rna in lean and obese pigs
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522191/
https://www.ncbi.nlm.nih.gov/pubmed/28589911
http://dx.doi.org/10.18632/oncotarget.18269
work_keys_str_mv AT yulin comparativeanalysesoflongnoncodingrnainleanandobesepigs
AT tailina comparativeanalysesoflongnoncodingrnainleanandobesepigs
AT zhanglifang comparativeanalysesoflongnoncodingrnainleanandobesepigs
AT chuyi comparativeanalysesoflongnoncodingrnainleanandobesepigs
AT liyixing comparativeanalysesoflongnoncodingrnainleanandobesepigs
AT zhoulei comparativeanalysesoflongnoncodingrnainleanandobesepigs