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Ephrin-B2/Fc promotes proliferation and migration, and suppresses apoptosis in human umbilical vein endothelial cells

Tumor growth and metastasis are angiogenesis dependent. Angiogenic growth involves endothelial cell proliferation, migration, and invasion. Ephrin-B2 is a ligand for Eph receptor tyrosine kinases and is an important mediator in vascular endothelial growth factor-mediated angiogenesis. However, resea...

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Autores principales: Zheng, Li-Chun, Wang, Xiao-Qing, Lu, Kun, Deng, Xiao-Ling, Zhang, Cheng-Wei, Luo, Hong, Xu, Xu-Dong, Chen, Xiao-Man, Yan, Lu, Wang, Yi-Qing, Shi, Song-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522204/
https://www.ncbi.nlm.nih.gov/pubmed/28489586
http://dx.doi.org/10.18632/oncotarget.17298
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author Zheng, Li-Chun
Wang, Xiao-Qing
Lu, Kun
Deng, Xiao-Ling
Zhang, Cheng-Wei
Luo, Hong
Xu, Xu-Dong
Chen, Xiao-Man
Yan, Lu
Wang, Yi-Qing
Shi, Song-Lin
author_facet Zheng, Li-Chun
Wang, Xiao-Qing
Lu, Kun
Deng, Xiao-Ling
Zhang, Cheng-Wei
Luo, Hong
Xu, Xu-Dong
Chen, Xiao-Man
Yan, Lu
Wang, Yi-Qing
Shi, Song-Lin
author_sort Zheng, Li-Chun
collection PubMed
description Tumor growth and metastasis are angiogenesis dependent. Angiogenic growth involves endothelial cell proliferation, migration, and invasion. Ephrin-B2 is a ligand for Eph receptor tyrosine kinases and is an important mediator in vascular endothelial growth factor-mediated angiogenesis. However, research offer controversial information regarding effects of ephrin-B2 on vascular endothelial cells. In this paper, proteome analyses showed that ephrin-B2/Fc significantly activates multiple signaling pathways related to cell proliferation, survival, and migration and suppresses apoptosis and cell death. Cytological experiments further confirm that ephrin-B2/Fc stimulates endothelial cell proliferation, triggers dose-dependent migration, and suppresses cell apoptosis. Results demonstrate that soluble dose-dependent ephrinB2 can promote proliferation and migration and inhibit apoptosis of human umbilical vein endothelial cells. These results also suggest that ephrinB2 prevents ischemic disease and can potentially be a new therapeutic target for treating angiogenesis-related diseases and tumors.
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spelling pubmed-55222042017-08-21 Ephrin-B2/Fc promotes proliferation and migration, and suppresses apoptosis in human umbilical vein endothelial cells Zheng, Li-Chun Wang, Xiao-Qing Lu, Kun Deng, Xiao-Ling Zhang, Cheng-Wei Luo, Hong Xu, Xu-Dong Chen, Xiao-Man Yan, Lu Wang, Yi-Qing Shi, Song-Lin Oncotarget Research Paper Tumor growth and metastasis are angiogenesis dependent. Angiogenic growth involves endothelial cell proliferation, migration, and invasion. Ephrin-B2 is a ligand for Eph receptor tyrosine kinases and is an important mediator in vascular endothelial growth factor-mediated angiogenesis. However, research offer controversial information regarding effects of ephrin-B2 on vascular endothelial cells. In this paper, proteome analyses showed that ephrin-B2/Fc significantly activates multiple signaling pathways related to cell proliferation, survival, and migration and suppresses apoptosis and cell death. Cytological experiments further confirm that ephrin-B2/Fc stimulates endothelial cell proliferation, triggers dose-dependent migration, and suppresses cell apoptosis. Results demonstrate that soluble dose-dependent ephrinB2 can promote proliferation and migration and inhibit apoptosis of human umbilical vein endothelial cells. These results also suggest that ephrinB2 prevents ischemic disease and can potentially be a new therapeutic target for treating angiogenesis-related diseases and tumors. Impact Journals LLC 2017-04-20 /pmc/articles/PMC5522204/ /pubmed/28489586 http://dx.doi.org/10.18632/oncotarget.17298 Text en Copyright: © 2017 Zheng et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Zheng, Li-Chun
Wang, Xiao-Qing
Lu, Kun
Deng, Xiao-Ling
Zhang, Cheng-Wei
Luo, Hong
Xu, Xu-Dong
Chen, Xiao-Man
Yan, Lu
Wang, Yi-Qing
Shi, Song-Lin
Ephrin-B2/Fc promotes proliferation and migration, and suppresses apoptosis in human umbilical vein endothelial cells
title Ephrin-B2/Fc promotes proliferation and migration, and suppresses apoptosis in human umbilical vein endothelial cells
title_full Ephrin-B2/Fc promotes proliferation and migration, and suppresses apoptosis in human umbilical vein endothelial cells
title_fullStr Ephrin-B2/Fc promotes proliferation and migration, and suppresses apoptosis in human umbilical vein endothelial cells
title_full_unstemmed Ephrin-B2/Fc promotes proliferation and migration, and suppresses apoptosis in human umbilical vein endothelial cells
title_short Ephrin-B2/Fc promotes proliferation and migration, and suppresses apoptosis in human umbilical vein endothelial cells
title_sort ephrin-b2/fc promotes proliferation and migration, and suppresses apoptosis in human umbilical vein endothelial cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522204/
https://www.ncbi.nlm.nih.gov/pubmed/28489586
http://dx.doi.org/10.18632/oncotarget.17298
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