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RACK1 promotes lung cancer cell growth via an MCM7/RACK1/Akt signaling complex
MCM7, a member of the miniature chromosome maintenance (MCM) protein family, is crucial for the initiation of DNA replication and proliferation in eukaryotic cells. In this report, we demonstrate that RACK1 regulates cell growth and cell cycle progression in human non-small-cell lung cancer by media...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522230/ https://www.ncbi.nlm.nih.gov/pubmed/28465488 http://dx.doi.org/10.18632/oncotarget.17120 |
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author | Fei, Liangru Ma, Yinan Zhang, Meiyu Liu, Xiaofang Luo, Yuan Wang, Congcong Zhang, Haiyan Zhang, Wenzhu Han, Yuchen |
author_facet | Fei, Liangru Ma, Yinan Zhang, Meiyu Liu, Xiaofang Luo, Yuan Wang, Congcong Zhang, Haiyan Zhang, Wenzhu Han, Yuchen |
author_sort | Fei, Liangru |
collection | PubMed |
description | MCM7, a member of the miniature chromosome maintenance (MCM) protein family, is crucial for the initiation of DNA replication and proliferation in eukaryotic cells. In this report, we demonstrate that RACK1 regulates cell growth and cell cycle progression in human non-small-cell lung cancer by mediating MCM7 phosphorylation through an MCM7/RACK1/Akt signaling complex. RACK1 functions as a central scaffold that brings Akt into physical proximity with MCM7. Overexpression of RACK1 increases interactions between Akt and MCM7 and promotes Akt-dependent MCM7 phosphorylation, which in turn increases MCM7 binding to chromatin and MCM complex formation. Together, these changes promote DNA replication and cell proliferation. Our findings reveal a novel signaling pathway that regulates growth in non-small cell lung cancer. |
format | Online Article Text |
id | pubmed-5522230 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-55222302017-08-21 RACK1 promotes lung cancer cell growth via an MCM7/RACK1/Akt signaling complex Fei, Liangru Ma, Yinan Zhang, Meiyu Liu, Xiaofang Luo, Yuan Wang, Congcong Zhang, Haiyan Zhang, Wenzhu Han, Yuchen Oncotarget Research Paper MCM7, a member of the miniature chromosome maintenance (MCM) protein family, is crucial for the initiation of DNA replication and proliferation in eukaryotic cells. In this report, we demonstrate that RACK1 regulates cell growth and cell cycle progression in human non-small-cell lung cancer by mediating MCM7 phosphorylation through an MCM7/RACK1/Akt signaling complex. RACK1 functions as a central scaffold that brings Akt into physical proximity with MCM7. Overexpression of RACK1 increases interactions between Akt and MCM7 and promotes Akt-dependent MCM7 phosphorylation, which in turn increases MCM7 binding to chromatin and MCM complex formation. Together, these changes promote DNA replication and cell proliferation. Our findings reveal a novel signaling pathway that regulates growth in non-small cell lung cancer. Impact Journals LLC 2017-04-15 /pmc/articles/PMC5522230/ /pubmed/28465488 http://dx.doi.org/10.18632/oncotarget.17120 Text en Copyright: © 2017 Fei et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Fei, Liangru Ma, Yinan Zhang, Meiyu Liu, Xiaofang Luo, Yuan Wang, Congcong Zhang, Haiyan Zhang, Wenzhu Han, Yuchen RACK1 promotes lung cancer cell growth via an MCM7/RACK1/Akt signaling complex |
title | RACK1 promotes lung cancer cell growth via an MCM7/RACK1/Akt signaling complex |
title_full | RACK1 promotes lung cancer cell growth via an MCM7/RACK1/Akt signaling complex |
title_fullStr | RACK1 promotes lung cancer cell growth via an MCM7/RACK1/Akt signaling complex |
title_full_unstemmed | RACK1 promotes lung cancer cell growth via an MCM7/RACK1/Akt signaling complex |
title_short | RACK1 promotes lung cancer cell growth via an MCM7/RACK1/Akt signaling complex |
title_sort | rack1 promotes lung cancer cell growth via an mcm7/rack1/akt signaling complex |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522230/ https://www.ncbi.nlm.nih.gov/pubmed/28465488 http://dx.doi.org/10.18632/oncotarget.17120 |
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