Cargando…

High-fidelity de novo synthesis of pathways using microchip-synthesized oligonucleotides and general molecular biology equipment

Engineering and evaluation of synthetic routes for generating valuable compounds require accurate and cost-effective de novo synthesis of genetic pathways. Here, we present an economical and streamlined de novo DNA synthesis approach for engineering a synthetic pathway with microchip-synthesized oli...

Descripción completa

Detalles Bibliográficos
Autores principales: Wan, Wen, Lu, Min, Wang, Dongmei, Gao, Xiaolian, Hong, Jiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522410/
https://www.ncbi.nlm.nih.gov/pubmed/28733633
http://dx.doi.org/10.1038/s41598-017-06428-0