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High-fidelity de novo synthesis of pathways using microchip-synthesized oligonucleotides and general molecular biology equipment
Engineering and evaluation of synthetic routes for generating valuable compounds require accurate and cost-effective de novo synthesis of genetic pathways. Here, we present an economical and streamlined de novo DNA synthesis approach for engineering a synthetic pathway with microchip-synthesized oli...
Autores principales: | Wan, Wen, Lu, Min, Wang, Dongmei, Gao, Xiaolian, Hong, Jiong |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522410/ https://www.ncbi.nlm.nih.gov/pubmed/28733633 http://dx.doi.org/10.1038/s41598-017-06428-0 |
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