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Modulation of water diffusion by activation-induced neural cell swelling in Aplysia Californica
Diffusion functional magnetic resonance imaging (DfMRI) has been proposed as a method for functional neuroimaging studies, as an alternative to blood oxygenation level dependent (BOLD)-fMRI. DfMRI is thought to more directly reflect neural activation, but its exact mechanism remains unclear. It has...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522485/ https://www.ncbi.nlm.nih.gov/pubmed/28733682 http://dx.doi.org/10.1038/s41598-017-05586-5 |
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author | Abe, Yoshifumi Van Nguyen, Khieu Tsurugizawa, Tomokazu Ciobanu, Luisa Le Bihan, Denis |
author_facet | Abe, Yoshifumi Van Nguyen, Khieu Tsurugizawa, Tomokazu Ciobanu, Luisa Le Bihan, Denis |
author_sort | Abe, Yoshifumi |
collection | PubMed |
description | Diffusion functional magnetic resonance imaging (DfMRI) has been proposed as a method for functional neuroimaging studies, as an alternative to blood oxygenation level dependent (BOLD)-fMRI. DfMRI is thought to more directly reflect neural activation, but its exact mechanism remains unclear. It has been hypothesized that the water apparent diffusion coefficient (ADC) decrease observed upon neural activation results from swelling of neurons or neuron parts. To elucidate the origin of the DfMRI response at cellular level we performed diffusion MR microscopy at 17.2 T in Aplysia californica buccal ganglia and compared the water ADCs at cellular and ganglia levels before and after neuronal activation induced by perfusion with a solution containing dopamine. Neural cell swelling, evidenced from optical microscopy imaging, resulted in an intracellular ADC increase and an ADC decrease at ganglia level. Furthermore, the intracellular ADC increase was found to have a significant positive correlation with the increase in cell size. Our results strongly support the hypothesis that the ADC decrease observed with DfMRI upon neuronal activation at tissue level reflects activation-induced neural cell swelling. |
format | Online Article Text |
id | pubmed-5522485 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55224852017-07-26 Modulation of water diffusion by activation-induced neural cell swelling in Aplysia Californica Abe, Yoshifumi Van Nguyen, Khieu Tsurugizawa, Tomokazu Ciobanu, Luisa Le Bihan, Denis Sci Rep Article Diffusion functional magnetic resonance imaging (DfMRI) has been proposed as a method for functional neuroimaging studies, as an alternative to blood oxygenation level dependent (BOLD)-fMRI. DfMRI is thought to more directly reflect neural activation, but its exact mechanism remains unclear. It has been hypothesized that the water apparent diffusion coefficient (ADC) decrease observed upon neural activation results from swelling of neurons or neuron parts. To elucidate the origin of the DfMRI response at cellular level we performed diffusion MR microscopy at 17.2 T in Aplysia californica buccal ganglia and compared the water ADCs at cellular and ganglia levels before and after neuronal activation induced by perfusion with a solution containing dopamine. Neural cell swelling, evidenced from optical microscopy imaging, resulted in an intracellular ADC increase and an ADC decrease at ganglia level. Furthermore, the intracellular ADC increase was found to have a significant positive correlation with the increase in cell size. Our results strongly support the hypothesis that the ADC decrease observed with DfMRI upon neuronal activation at tissue level reflects activation-induced neural cell swelling. Nature Publishing Group UK 2017-07-21 /pmc/articles/PMC5522485/ /pubmed/28733682 http://dx.doi.org/10.1038/s41598-017-05586-5 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Abe, Yoshifumi Van Nguyen, Khieu Tsurugizawa, Tomokazu Ciobanu, Luisa Le Bihan, Denis Modulation of water diffusion by activation-induced neural cell swelling in Aplysia Californica |
title | Modulation of water diffusion by activation-induced neural cell swelling in Aplysia Californica |
title_full | Modulation of water diffusion by activation-induced neural cell swelling in Aplysia Californica |
title_fullStr | Modulation of water diffusion by activation-induced neural cell swelling in Aplysia Californica |
title_full_unstemmed | Modulation of water diffusion by activation-induced neural cell swelling in Aplysia Californica |
title_short | Modulation of water diffusion by activation-induced neural cell swelling in Aplysia Californica |
title_sort | modulation of water diffusion by activation-induced neural cell swelling in aplysia californica |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522485/ https://www.ncbi.nlm.nih.gov/pubmed/28733682 http://dx.doi.org/10.1038/s41598-017-05586-5 |
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