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Tetracycline Resistance Genes Identified from Distinct Soil Environments in China by Functional Metagenomics

Soil microbiota represents one of the ancient evolutionary origins of antibiotic resistance and has been increasingly recognized as a potentially vast unstudied reservoir of resistance genes with possibilities to exchange with pathogens. Tetracycline resistance is one of the most abundant antibiotic...

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Autores principales: Wang, Shaochen, Gao, Xia, Gao, Yuejiao, Li, Yanqing, Cao, Mingming, Xi, Zhenhua, Zhao, Lixing, Feng, Zhiyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522880/
https://www.ncbi.nlm.nih.gov/pubmed/28790997
http://dx.doi.org/10.3389/fmicb.2017.01406
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author Wang, Shaochen
Gao, Xia
Gao, Yuejiao
Li, Yanqing
Cao, Mingming
Xi, Zhenhua
Zhao, Lixing
Feng, Zhiyang
author_facet Wang, Shaochen
Gao, Xia
Gao, Yuejiao
Li, Yanqing
Cao, Mingming
Xi, Zhenhua
Zhao, Lixing
Feng, Zhiyang
author_sort Wang, Shaochen
collection PubMed
description Soil microbiota represents one of the ancient evolutionary origins of antibiotic resistance and has been increasingly recognized as a potentially vast unstudied reservoir of resistance genes with possibilities to exchange with pathogens. Tetracycline resistance is one of the most abundant antibiotic resistances that may transfer among clinical and commensal microorganisms. To investigate tetracycline resistance genes from soil bacteria in different habitats, we performed functional analysis of three metagenomic libraries derived from soil samples collected from Yunnan, Sichuan, and Tibet, respectively, in China. We found efflux transporter genes form all the libraries, including 21 major facilitator superfamily efflux pump genes and one multidrug and toxic compound extrusion (MATE) transporter gene. Interestingly, we also identified two tetracycline destructase genes, belonging to a newly described family of tetracycline-inactivating enzymes that scarcely observed in clinical pathogens, from the Tibet library. The inactivation activity of the putative enzyme was confirmed in vitro by biochemical analysis. Our results indicated that efflux pumps distributed predominantly across habitats. Meanwhile, the mechanism of enzymatic inactivation for tetracycline resistance should not be neglected and merits further investigation.
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spelling pubmed-55228802017-08-08 Tetracycline Resistance Genes Identified from Distinct Soil Environments in China by Functional Metagenomics Wang, Shaochen Gao, Xia Gao, Yuejiao Li, Yanqing Cao, Mingming Xi, Zhenhua Zhao, Lixing Feng, Zhiyang Front Microbiol Microbiology Soil microbiota represents one of the ancient evolutionary origins of antibiotic resistance and has been increasingly recognized as a potentially vast unstudied reservoir of resistance genes with possibilities to exchange with pathogens. Tetracycline resistance is one of the most abundant antibiotic resistances that may transfer among clinical and commensal microorganisms. To investigate tetracycline resistance genes from soil bacteria in different habitats, we performed functional analysis of three metagenomic libraries derived from soil samples collected from Yunnan, Sichuan, and Tibet, respectively, in China. We found efflux transporter genes form all the libraries, including 21 major facilitator superfamily efflux pump genes and one multidrug and toxic compound extrusion (MATE) transporter gene. Interestingly, we also identified two tetracycline destructase genes, belonging to a newly described family of tetracycline-inactivating enzymes that scarcely observed in clinical pathogens, from the Tibet library. The inactivation activity of the putative enzyme was confirmed in vitro by biochemical analysis. Our results indicated that efflux pumps distributed predominantly across habitats. Meanwhile, the mechanism of enzymatic inactivation for tetracycline resistance should not be neglected and merits further investigation. Frontiers Media S.A. 2017-07-24 /pmc/articles/PMC5522880/ /pubmed/28790997 http://dx.doi.org/10.3389/fmicb.2017.01406 Text en Copyright © 2017 Wang, Gao, Gao, Li, Cao, Xi, Zhao and Feng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wang, Shaochen
Gao, Xia
Gao, Yuejiao
Li, Yanqing
Cao, Mingming
Xi, Zhenhua
Zhao, Lixing
Feng, Zhiyang
Tetracycline Resistance Genes Identified from Distinct Soil Environments in China by Functional Metagenomics
title Tetracycline Resistance Genes Identified from Distinct Soil Environments in China by Functional Metagenomics
title_full Tetracycline Resistance Genes Identified from Distinct Soil Environments in China by Functional Metagenomics
title_fullStr Tetracycline Resistance Genes Identified from Distinct Soil Environments in China by Functional Metagenomics
title_full_unstemmed Tetracycline Resistance Genes Identified from Distinct Soil Environments in China by Functional Metagenomics
title_short Tetracycline Resistance Genes Identified from Distinct Soil Environments in China by Functional Metagenomics
title_sort tetracycline resistance genes identified from distinct soil environments in china by functional metagenomics
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522880/
https://www.ncbi.nlm.nih.gov/pubmed/28790997
http://dx.doi.org/10.3389/fmicb.2017.01406
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