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Focal Defects of the Knee Articular Surface: Evidence of a Regenerative Potential Pattern in Osteochondritis Dissecans and Degenerative Lesions

The surgical treatment of knee articular focal lesions may offer heterogeneous clinical results. Osteochondritis dissecans (OCD) lesions showed to heal better than degenerative lesions (DL) but the underlying biological reasons are unknown. We evaluated the basal histological and immunohistochemical...

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Autores principales: Gabusi, Elena, Manferdini, Cristina, Paolella, Francesca, Gambari, Laura, Kon, Elizaveta, Filardo, Giuseppe, Mariani, Erminia, Lisignoli, Gina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5523180/
https://www.ncbi.nlm.nih.gov/pubmed/28770227
http://dx.doi.org/10.1155/2017/9036305
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author Gabusi, Elena
Manferdini, Cristina
Paolella, Francesca
Gambari, Laura
Kon, Elizaveta
Filardo, Giuseppe
Mariani, Erminia
Lisignoli, Gina
author_facet Gabusi, Elena
Manferdini, Cristina
Paolella, Francesca
Gambari, Laura
Kon, Elizaveta
Filardo, Giuseppe
Mariani, Erminia
Lisignoli, Gina
author_sort Gabusi, Elena
collection PubMed
description The surgical treatment of knee articular focal lesions may offer heterogeneous clinical results. Osteochondritis dissecans (OCD) lesions showed to heal better than degenerative lesions (DL) but the underlying biological reasons are unknown. We evaluated the basal histological and immunohistochemical characteristics of these lesions analyzing a series of osteochondral fragments from young patients with similar age but presenting different etiology. Osteochondral tissue samples were stained with Safranin O and graded using a histological score. Markers of mesenchymal progenitor cells (CD146), osteoclasts (tartrate-resistant acid phosphatase, TRAP), and vessels (CD34) were evaluated. Histological score showed a higher degeneration of both cartilage and bone compartments in OCD compared to DL fragments. Only CD146-positive cells were found at the same percentage in cartilage compartment of both DL and OCD patients. By contrast, in the bone compartment a significantly higher percentage of CD146, TRAP, and CD34 markers was found in OCD compared to DL patients. These data showed distinct histological characteristics of osteochondral focal lesions located in the same anatomical region but having a different etiology. The higher percentages of these markers in OCD than in DL, mainly associated with a high bone turnover, could help to explain the higher clinical healing potential of OCD patients.
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spelling pubmed-55231802017-08-02 Focal Defects of the Knee Articular Surface: Evidence of a Regenerative Potential Pattern in Osteochondritis Dissecans and Degenerative Lesions Gabusi, Elena Manferdini, Cristina Paolella, Francesca Gambari, Laura Kon, Elizaveta Filardo, Giuseppe Mariani, Erminia Lisignoli, Gina Biomed Res Int Research Article The surgical treatment of knee articular focal lesions may offer heterogeneous clinical results. Osteochondritis dissecans (OCD) lesions showed to heal better than degenerative lesions (DL) but the underlying biological reasons are unknown. We evaluated the basal histological and immunohistochemical characteristics of these lesions analyzing a series of osteochondral fragments from young patients with similar age but presenting different etiology. Osteochondral tissue samples were stained with Safranin O and graded using a histological score. Markers of mesenchymal progenitor cells (CD146), osteoclasts (tartrate-resistant acid phosphatase, TRAP), and vessels (CD34) were evaluated. Histological score showed a higher degeneration of both cartilage and bone compartments in OCD compared to DL fragments. Only CD146-positive cells were found at the same percentage in cartilage compartment of both DL and OCD patients. By contrast, in the bone compartment a significantly higher percentage of CD146, TRAP, and CD34 markers was found in OCD compared to DL patients. These data showed distinct histological characteristics of osteochondral focal lesions located in the same anatomical region but having a different etiology. The higher percentages of these markers in OCD than in DL, mainly associated with a high bone turnover, could help to explain the higher clinical healing potential of OCD patients. Hindawi 2017 2017-07-09 /pmc/articles/PMC5523180/ /pubmed/28770227 http://dx.doi.org/10.1155/2017/9036305 Text en Copyright © 2017 Elena Gabusi et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gabusi, Elena
Manferdini, Cristina
Paolella, Francesca
Gambari, Laura
Kon, Elizaveta
Filardo, Giuseppe
Mariani, Erminia
Lisignoli, Gina
Focal Defects of the Knee Articular Surface: Evidence of a Regenerative Potential Pattern in Osteochondritis Dissecans and Degenerative Lesions
title Focal Defects of the Knee Articular Surface: Evidence of a Regenerative Potential Pattern in Osteochondritis Dissecans and Degenerative Lesions
title_full Focal Defects of the Knee Articular Surface: Evidence of a Regenerative Potential Pattern in Osteochondritis Dissecans and Degenerative Lesions
title_fullStr Focal Defects of the Knee Articular Surface: Evidence of a Regenerative Potential Pattern in Osteochondritis Dissecans and Degenerative Lesions
title_full_unstemmed Focal Defects of the Knee Articular Surface: Evidence of a Regenerative Potential Pattern in Osteochondritis Dissecans and Degenerative Lesions
title_short Focal Defects of the Knee Articular Surface: Evidence of a Regenerative Potential Pattern in Osteochondritis Dissecans and Degenerative Lesions
title_sort focal defects of the knee articular surface: evidence of a regenerative potential pattern in osteochondritis dissecans and degenerative lesions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5523180/
https://www.ncbi.nlm.nih.gov/pubmed/28770227
http://dx.doi.org/10.1155/2017/9036305
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