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Acceleration of the sliding movement of actin filaments with the use of a non-motile mutant myosin in in vitro motility assays driven by skeletal muscle heavy meromyosin
We examined the movement of an actin filament sliding on a mixture of normal and genetically modified myosin molecules that were attached to a glass surface. For this purpose, we used a Dictyostelium G680V mutant myosin II whose release rates of Pi and ADP were highly suppressed relative to normal m...
Autores principales: | Iwase, Kohei, Tanaka, Masateru, Hirose, Keiko, Uyeda, Taro Q. P., Honda, Hajime |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5524339/ https://www.ncbi.nlm.nih.gov/pubmed/28742155 http://dx.doi.org/10.1371/journal.pone.0181171 |
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