Cargando…
Fingerprint analysis of Resina Draconis by ultra-performance liquid chromatography
BACKGROUND: Resina Draconis, a bright red resin derived from Dracaena cochinchinensis, is a traditional medicine used in China. To improve its quality control approach, an ultra-performance liquid chromatography (UPLC) fingerprint method was developed for rapidly evaluating the quality of Resina Dra...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5524661/ https://www.ncbi.nlm.nih.gov/pubmed/29086860 http://dx.doi.org/10.1186/s13065-017-0299-8 |
_version_ | 1783252491924668416 |
---|---|
author | Xue, Yudi Zhu, Lin Yi, Tao |
author_facet | Xue, Yudi Zhu, Lin Yi, Tao |
author_sort | Xue, Yudi |
collection | PubMed |
description | BACKGROUND: Resina Draconis, a bright red resin derived from Dracaena cochinchinensis, is a traditional medicine used in China. To improve its quality control approach, an ultra-performance liquid chromatography (UPLC) fingerprint method was developed for rapidly evaluating the quality of Resina Draconis. METHODS: The precision, repeatability and stability of the proposed UPLC method were validated in the study. Twelve batches of Resina Draconis samples from various sources were analyzed by the present UPLC method. Common peaks in the chromatograms were adopted to calculate their relative retention time and relative peak area. The chromatographic data were processed by Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine software (Version 2004 A) for similarity analysis. RESULTS: The present UPLC method demonstrated a satisfactory precision, repeatability and stability. The analysis time of the present UPLC method was shortened to 30 min, compared with that of the conventional HPLC method was 50 min. The similarities of the 12 Resina Draconis samples were 0.976, 0.993, 0.955, 0.789, 0.989, 0.995, 0.794, 0.994, 0.847, 0.987, 0.997, 0.986, respectively, which indicated that the samples were certainly regionally different. The similarities of the 12 samples showed more similar pattern except for samples 4, 7 and 9. Such variation in similarity may presumably be attributed to differences in source. CONCLUSIONS: Compared with the conventional HPLC method, the present UPLC method showed several advantages including shorter analysis time, higher resolution and better separation performance. The UPLC fingerprinting established in the present paper provides a valuable reference for the quality control of Resina Draconis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13065-017-0299-8) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5524661 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-55246612017-08-08 Fingerprint analysis of Resina Draconis by ultra-performance liquid chromatography Xue, Yudi Zhu, Lin Yi, Tao Chem Cent J Research Article BACKGROUND: Resina Draconis, a bright red resin derived from Dracaena cochinchinensis, is a traditional medicine used in China. To improve its quality control approach, an ultra-performance liquid chromatography (UPLC) fingerprint method was developed for rapidly evaluating the quality of Resina Draconis. METHODS: The precision, repeatability and stability of the proposed UPLC method were validated in the study. Twelve batches of Resina Draconis samples from various sources were analyzed by the present UPLC method. Common peaks in the chromatograms were adopted to calculate their relative retention time and relative peak area. The chromatographic data were processed by Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine software (Version 2004 A) for similarity analysis. RESULTS: The present UPLC method demonstrated a satisfactory precision, repeatability and stability. The analysis time of the present UPLC method was shortened to 30 min, compared with that of the conventional HPLC method was 50 min. The similarities of the 12 Resina Draconis samples were 0.976, 0.993, 0.955, 0.789, 0.989, 0.995, 0.794, 0.994, 0.847, 0.987, 0.997, 0.986, respectively, which indicated that the samples were certainly regionally different. The similarities of the 12 samples showed more similar pattern except for samples 4, 7 and 9. Such variation in similarity may presumably be attributed to differences in source. CONCLUSIONS: Compared with the conventional HPLC method, the present UPLC method showed several advantages including shorter analysis time, higher resolution and better separation performance. The UPLC fingerprinting established in the present paper provides a valuable reference for the quality control of Resina Draconis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13065-017-0299-8) contains supplementary material, which is available to authorized users. Springer International Publishing 2017-07-24 /pmc/articles/PMC5524661/ /pubmed/29086860 http://dx.doi.org/10.1186/s13065-017-0299-8 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Xue, Yudi Zhu, Lin Yi, Tao Fingerprint analysis of Resina Draconis by ultra-performance liquid chromatography |
title | Fingerprint analysis of Resina Draconis by ultra-performance liquid chromatography |
title_full | Fingerprint analysis of Resina Draconis by ultra-performance liquid chromatography |
title_fullStr | Fingerprint analysis of Resina Draconis by ultra-performance liquid chromatography |
title_full_unstemmed | Fingerprint analysis of Resina Draconis by ultra-performance liquid chromatography |
title_short | Fingerprint analysis of Resina Draconis by ultra-performance liquid chromatography |
title_sort | fingerprint analysis of resina draconis by ultra-performance liquid chromatography |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5524661/ https://www.ncbi.nlm.nih.gov/pubmed/29086860 http://dx.doi.org/10.1186/s13065-017-0299-8 |
work_keys_str_mv | AT xueyudi fingerprintanalysisofresinadraconisbyultraperformanceliquidchromatography AT zhulin fingerprintanalysisofresinadraconisbyultraperformanceliquidchromatography AT yitao fingerprintanalysisofresinadraconisbyultraperformanceliquidchromatography |