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Dual functional Phi29 DNA polymerase-triggered exponential rolling circle amplification for sequence-specific detection of target DNA embedded in long-stranded genomic DNA

An exonucleolytic digestion-assisted exponential rolling circle amplification (RCA) strategy was developed for sensitive and sequence-specific detection of target DNA embedded in long-stranded genomic DNA. Herein, Phi29 DNA polymerase plays two important roles as exonuclease and polymerase. Long-str...

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Detalles Bibliográficos
Autores principales: Li, Xiao-Yu, Du, Yi-Chen, Zhang, Yu-Peng, Kong, De-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5524717/
https://www.ncbi.nlm.nih.gov/pubmed/28740223
http://dx.doi.org/10.1038/s41598-017-06594-1