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Dual functional Phi29 DNA polymerase-triggered exponential rolling circle amplification for sequence-specific detection of target DNA embedded in long-stranded genomic DNA
An exonucleolytic digestion-assisted exponential rolling circle amplification (RCA) strategy was developed for sensitive and sequence-specific detection of target DNA embedded in long-stranded genomic DNA. Herein, Phi29 DNA polymerase plays two important roles as exonuclease and polymerase. Long-str...
Autores principales: | Li, Xiao-Yu, Du, Yi-Chen, Zhang, Yu-Peng, Kong, De-Ming |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5524717/ https://www.ncbi.nlm.nih.gov/pubmed/28740223 http://dx.doi.org/10.1038/s41598-017-06594-1 |
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