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In vivo imaging to monitor differentiation and therapeutic effects of transplanted mesenchymal stem cells in myocardial infarction
Here, we used a noninvasive multimodality imaging approach to monitor differentiation of transplanted bone marrow mesenchymal stem cells (BMSCs) and recovery of cardiac function in an in vivo model of myocardial infarction (MI). We established a rat MI model by coronary artery ligation. Ninety rats...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5524783/ https://www.ncbi.nlm.nih.gov/pubmed/28740146 http://dx.doi.org/10.1038/s41598-017-06571-8 |
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author | Pei, Zhijun Zeng, Jing Song, Yafeng Gao, Yan Wu, Ruimin Chen, Yijia Li, Fuyan Li, Wei Zhou, Hong Yang, Yi |
author_facet | Pei, Zhijun Zeng, Jing Song, Yafeng Gao, Yan Wu, Ruimin Chen, Yijia Li, Fuyan Li, Wei Zhou, Hong Yang, Yi |
author_sort | Pei, Zhijun |
collection | PubMed |
description | Here, we used a noninvasive multimodality imaging approach to monitor differentiation of transplanted bone marrow mesenchymal stem cells (BMSCs) and recovery of cardiac function in an in vivo model of myocardial infarction (MI). We established a rat MI model by coronary artery ligation. Ninety rats were randomly assigned into four groups: sham-operated, MI model, and α-MHC-HSV1-tk-transfected or un-transfected BMSCs-treated MI model. We used (18)F-Fluro-deoxyglucose ((18)F-FDG) positron emission tomography (PET) to monitor recovery of cardiac function, and (18)F-FHBG PET/CT imaging to monitor transplanted BMSCs differentiation 24 h after (18)F-FDG imaging. The uptake of (18)F-FDG at 3, 16, 30 and 45 days after BMSCs injection was 0.39 ± 0.03, 0.57 ± 0.05, 0.59 ± 0.04, and 0.71 ± 0.05% ID/g, respectively. Uptake of (18)F-FHBG increased significantly in large areas in the BMSCs-treated group over time. Ex vivo experiments indicated that expression of the cardiomyocyte markers GATA-4 and cardiac troponin I markedly increased in the BMSCs-treated group. Additionally, immunohistochemistry revealed that HSV-tk-labelled BMSCs-derived cells were positive for cardiac troponin I. Multimodal imaging systems combining an α-MHC-HSV1-tk/(18)F-FHBG reporter gene and (18)F-FDG metabolism imaging could be used to track differentiation of transplanted BMSCs and recovery of cardiac function in MI. |
format | Online Article Text |
id | pubmed-5524783 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55247832017-07-26 In vivo imaging to monitor differentiation and therapeutic effects of transplanted mesenchymal stem cells in myocardial infarction Pei, Zhijun Zeng, Jing Song, Yafeng Gao, Yan Wu, Ruimin Chen, Yijia Li, Fuyan Li, Wei Zhou, Hong Yang, Yi Sci Rep Article Here, we used a noninvasive multimodality imaging approach to monitor differentiation of transplanted bone marrow mesenchymal stem cells (BMSCs) and recovery of cardiac function in an in vivo model of myocardial infarction (MI). We established a rat MI model by coronary artery ligation. Ninety rats were randomly assigned into four groups: sham-operated, MI model, and α-MHC-HSV1-tk-transfected or un-transfected BMSCs-treated MI model. We used (18)F-Fluro-deoxyglucose ((18)F-FDG) positron emission tomography (PET) to monitor recovery of cardiac function, and (18)F-FHBG PET/CT imaging to monitor transplanted BMSCs differentiation 24 h after (18)F-FDG imaging. The uptake of (18)F-FDG at 3, 16, 30 and 45 days after BMSCs injection was 0.39 ± 0.03, 0.57 ± 0.05, 0.59 ± 0.04, and 0.71 ± 0.05% ID/g, respectively. Uptake of (18)F-FHBG increased significantly in large areas in the BMSCs-treated group over time. Ex vivo experiments indicated that expression of the cardiomyocyte markers GATA-4 and cardiac troponin I markedly increased in the BMSCs-treated group. Additionally, immunohistochemistry revealed that HSV-tk-labelled BMSCs-derived cells were positive for cardiac troponin I. Multimodal imaging systems combining an α-MHC-HSV1-tk/(18)F-FHBG reporter gene and (18)F-FDG metabolism imaging could be used to track differentiation of transplanted BMSCs and recovery of cardiac function in MI. Nature Publishing Group UK 2017-07-24 /pmc/articles/PMC5524783/ /pubmed/28740146 http://dx.doi.org/10.1038/s41598-017-06571-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Pei, Zhijun Zeng, Jing Song, Yafeng Gao, Yan Wu, Ruimin Chen, Yijia Li, Fuyan Li, Wei Zhou, Hong Yang, Yi In vivo imaging to monitor differentiation and therapeutic effects of transplanted mesenchymal stem cells in myocardial infarction |
title | In vivo imaging to monitor differentiation and therapeutic effects of transplanted mesenchymal stem cells in myocardial infarction |
title_full | In vivo imaging to monitor differentiation and therapeutic effects of transplanted mesenchymal stem cells in myocardial infarction |
title_fullStr | In vivo imaging to monitor differentiation and therapeutic effects of transplanted mesenchymal stem cells in myocardial infarction |
title_full_unstemmed | In vivo imaging to monitor differentiation and therapeutic effects of transplanted mesenchymal stem cells in myocardial infarction |
title_short | In vivo imaging to monitor differentiation and therapeutic effects of transplanted mesenchymal stem cells in myocardial infarction |
title_sort | in vivo imaging to monitor differentiation and therapeutic effects of transplanted mesenchymal stem cells in myocardial infarction |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5524783/ https://www.ncbi.nlm.nih.gov/pubmed/28740146 http://dx.doi.org/10.1038/s41598-017-06571-8 |
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