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Adaptation of the Pivotal-Differential Genome Pattern for the Induction of Intergenomic Chromosome Recombination in Hybrids of Synthetic Amphidiploids within Triticeae Tribe
A pivotal-differential evolution pattern is when two allopolyploids share a common genome, which is called pivotal, and differ with respect to the other genome or genomes, called differential. This feature induces the intergenomic recombination between chromosomes of differential genomes, which can...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5524833/ https://www.ncbi.nlm.nih.gov/pubmed/28791037 http://dx.doi.org/10.3389/fpls.2017.01300 |
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author | Kwiatek, Michal T. Majka, Joanna Majka, Maciej Belter, Jolanta Wisniewska, Halina |
author_facet | Kwiatek, Michal T. Majka, Joanna Majka, Maciej Belter, Jolanta Wisniewska, Halina |
author_sort | Kwiatek, Michal T. |
collection | PubMed |
description | A pivotal-differential evolution pattern is when two allopolyploids share a common genome, which is called pivotal, and differ with respect to the other genome or genomes, called differential. This feature induces the intergenomic recombination between chromosomes of differential genomes, which can lead to speciation. Our study is a cytomolecular insight into this mechanism which was adapted for the induction of intergenomic chromosome recombination in hybrids of synthetic amphidiploids Aegilops biuncialis × S. cereale (UUMMRR) and triticale (AABBRR) where R-genome was pivotal. We observed chromosome recombination events which were induced by both: (1) random chromosome fragmentation and non-homologous chromosome end joining at mitosis of root meristem cells and (2) intergenomic chromosome associations at meiosis of pollen mother cells (PMCs) of F(1) hybrids. Reciprocal chromosome translocations were identified in six F(1) plants and 15 plants of F(2) generation using fluorescence in situ hybridization (FISH) with DNA clones (pTa-86, pTa-k374, pTa-465, pTa-535, pTa-k566, and pTa-713). We observed signals of pTa-86, pTa-535, and pTa-k566 probes in several chromosome breakpoints. The comparison of the DNA clone sequences distinguished a number of common motifs, which can be considered as characteristics of chromosome breakpoint loci. Immunodetection of synaptonemal complex proteins and genomic in situ hybridization analysis at meiosis of PMCs of F(1) hybrids showed, that the homologous pairing of pivotal R—genome chromosomes is crucial for the fertility of F(1) hybrids, however, these chromosomes can be also involved in the intergeneric recombination. |
format | Online Article Text |
id | pubmed-5524833 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-55248332017-08-08 Adaptation of the Pivotal-Differential Genome Pattern for the Induction of Intergenomic Chromosome Recombination in Hybrids of Synthetic Amphidiploids within Triticeae Tribe Kwiatek, Michal T. Majka, Joanna Majka, Maciej Belter, Jolanta Wisniewska, Halina Front Plant Sci Plant Science A pivotal-differential evolution pattern is when two allopolyploids share a common genome, which is called pivotal, and differ with respect to the other genome or genomes, called differential. This feature induces the intergenomic recombination between chromosomes of differential genomes, which can lead to speciation. Our study is a cytomolecular insight into this mechanism which was adapted for the induction of intergenomic chromosome recombination in hybrids of synthetic amphidiploids Aegilops biuncialis × S. cereale (UUMMRR) and triticale (AABBRR) where R-genome was pivotal. We observed chromosome recombination events which were induced by both: (1) random chromosome fragmentation and non-homologous chromosome end joining at mitosis of root meristem cells and (2) intergenomic chromosome associations at meiosis of pollen mother cells (PMCs) of F(1) hybrids. Reciprocal chromosome translocations were identified in six F(1) plants and 15 plants of F(2) generation using fluorescence in situ hybridization (FISH) with DNA clones (pTa-86, pTa-k374, pTa-465, pTa-535, pTa-k566, and pTa-713). We observed signals of pTa-86, pTa-535, and pTa-k566 probes in several chromosome breakpoints. The comparison of the DNA clone sequences distinguished a number of common motifs, which can be considered as characteristics of chromosome breakpoint loci. Immunodetection of synaptonemal complex proteins and genomic in situ hybridization analysis at meiosis of PMCs of F(1) hybrids showed, that the homologous pairing of pivotal R—genome chromosomes is crucial for the fertility of F(1) hybrids, however, these chromosomes can be also involved in the intergeneric recombination. Frontiers Media S.A. 2017-07-25 /pmc/articles/PMC5524833/ /pubmed/28791037 http://dx.doi.org/10.3389/fpls.2017.01300 Text en Copyright © 2017 Kwiatek, Majka, Majka, Belter and Wisniewska. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Kwiatek, Michal T. Majka, Joanna Majka, Maciej Belter, Jolanta Wisniewska, Halina Adaptation of the Pivotal-Differential Genome Pattern for the Induction of Intergenomic Chromosome Recombination in Hybrids of Synthetic Amphidiploids within Triticeae Tribe |
title | Adaptation of the Pivotal-Differential Genome Pattern for the Induction of Intergenomic Chromosome Recombination in Hybrids of Synthetic Amphidiploids within Triticeae Tribe |
title_full | Adaptation of the Pivotal-Differential Genome Pattern for the Induction of Intergenomic Chromosome Recombination in Hybrids of Synthetic Amphidiploids within Triticeae Tribe |
title_fullStr | Adaptation of the Pivotal-Differential Genome Pattern for the Induction of Intergenomic Chromosome Recombination in Hybrids of Synthetic Amphidiploids within Triticeae Tribe |
title_full_unstemmed | Adaptation of the Pivotal-Differential Genome Pattern for the Induction of Intergenomic Chromosome Recombination in Hybrids of Synthetic Amphidiploids within Triticeae Tribe |
title_short | Adaptation of the Pivotal-Differential Genome Pattern for the Induction of Intergenomic Chromosome Recombination in Hybrids of Synthetic Amphidiploids within Triticeae Tribe |
title_sort | adaptation of the pivotal-differential genome pattern for the induction of intergenomic chromosome recombination in hybrids of synthetic amphidiploids within triticeae tribe |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5524833/ https://www.ncbi.nlm.nih.gov/pubmed/28791037 http://dx.doi.org/10.3389/fpls.2017.01300 |
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