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Avian influenza H9N2 virus isolated from air samples in LPMs in Jiangxi, China
BACKGROUND: Recently, avian influenza virus has caused repeated worldwide outbreaks in humans. Live Poultry Markets (LPMs) play an important role in the circulation and reassortment of novel Avian Influenza Virus (AIVs). Aerosol transmission is one of the most important pathways for influenza virus...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5525224/ https://www.ncbi.nlm.nih.gov/pubmed/28738865 http://dx.doi.org/10.1186/s12985-017-0800-y |
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author | Zeng, Xiaoxu Liu, Mingbin Zhang, Heng Wu, Jingwen Zhao, Xiang Chen, Wenbing Yang, Lei He, Fenglan Fan, Guoyin Wang, Dayan Chen, Haiying Shu, Yuelong |
author_facet | Zeng, Xiaoxu Liu, Mingbin Zhang, Heng Wu, Jingwen Zhao, Xiang Chen, Wenbing Yang, Lei He, Fenglan Fan, Guoyin Wang, Dayan Chen, Haiying Shu, Yuelong |
author_sort | Zeng, Xiaoxu |
collection | PubMed |
description | BACKGROUND: Recently, avian influenza virus has caused repeated worldwide outbreaks in humans. Live Poultry Markets (LPMs) play an important role in the circulation and reassortment of novel Avian Influenza Virus (AIVs). Aerosol transmission is one of the most important pathways for influenza virus to spread among poultry, from poultry to mammals, and among mammals. METHODS: In this study, air samples were collected from LPMs in Nanchang city between April 2014 and March 2015 to investigate possible aerosol transmission of AIVs. Air samples were detected for Flu A by Real-Time Reverse Transcription-Polymerase Chain Reaction (RRT-PCR). If samples were positive for Flu A, they were inoculated into 9- to 10-day-old specific-pathogen-free embryonated eggs. If the result was positive, the whole genome of the virus was sequenced by MiSeq. Phylogenetic trees of all 8 segments were constructed using MEGA 6.05 software. RESULTS: To investigate the possible aerosol transmission of AIVs, 807 air samples were collected from LPMs in Nanchang city between April 2014 and March 2015. Based on RRT-PCR results, 275 samples (34.1%) were Flu A positive, and one virus was successfully isolated with embryonated eggs. The virus shared high nucleotide homology with H9N2 AIVs from South China. CONCLUSIONS: Our study provides further evidence that the air in LPMs can be contaminated by influenza viruses and their nucleic acids, and this should be considered when choosing and evaluating disinfection strategies in LPMs, such as regular air disinfection. Aerosolized viruses such as the H9N2 virus detected in this study can increase the risk of human infection when people are exposed in LPMs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12985-017-0800-y) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5525224 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-55252242017-07-26 Avian influenza H9N2 virus isolated from air samples in LPMs in Jiangxi, China Zeng, Xiaoxu Liu, Mingbin Zhang, Heng Wu, Jingwen Zhao, Xiang Chen, Wenbing Yang, Lei He, Fenglan Fan, Guoyin Wang, Dayan Chen, Haiying Shu, Yuelong Virol J Research BACKGROUND: Recently, avian influenza virus has caused repeated worldwide outbreaks in humans. Live Poultry Markets (LPMs) play an important role in the circulation and reassortment of novel Avian Influenza Virus (AIVs). Aerosol transmission is one of the most important pathways for influenza virus to spread among poultry, from poultry to mammals, and among mammals. METHODS: In this study, air samples were collected from LPMs in Nanchang city between April 2014 and March 2015 to investigate possible aerosol transmission of AIVs. Air samples were detected for Flu A by Real-Time Reverse Transcription-Polymerase Chain Reaction (RRT-PCR). If samples were positive for Flu A, they were inoculated into 9- to 10-day-old specific-pathogen-free embryonated eggs. If the result was positive, the whole genome of the virus was sequenced by MiSeq. Phylogenetic trees of all 8 segments were constructed using MEGA 6.05 software. RESULTS: To investigate the possible aerosol transmission of AIVs, 807 air samples were collected from LPMs in Nanchang city between April 2014 and March 2015. Based on RRT-PCR results, 275 samples (34.1%) were Flu A positive, and one virus was successfully isolated with embryonated eggs. The virus shared high nucleotide homology with H9N2 AIVs from South China. CONCLUSIONS: Our study provides further evidence that the air in LPMs can be contaminated by influenza viruses and their nucleic acids, and this should be considered when choosing and evaluating disinfection strategies in LPMs, such as regular air disinfection. Aerosolized viruses such as the H9N2 virus detected in this study can increase the risk of human infection when people are exposed in LPMs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12985-017-0800-y) contains supplementary material, which is available to authorized users. BioMed Central 2017-07-24 /pmc/articles/PMC5525224/ /pubmed/28738865 http://dx.doi.org/10.1186/s12985-017-0800-y Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Zeng, Xiaoxu Liu, Mingbin Zhang, Heng Wu, Jingwen Zhao, Xiang Chen, Wenbing Yang, Lei He, Fenglan Fan, Guoyin Wang, Dayan Chen, Haiying Shu, Yuelong Avian influenza H9N2 virus isolated from air samples in LPMs in Jiangxi, China |
title | Avian influenza H9N2 virus isolated from air samples in LPMs in Jiangxi, China |
title_full | Avian influenza H9N2 virus isolated from air samples in LPMs in Jiangxi, China |
title_fullStr | Avian influenza H9N2 virus isolated from air samples in LPMs in Jiangxi, China |
title_full_unstemmed | Avian influenza H9N2 virus isolated from air samples in LPMs in Jiangxi, China |
title_short | Avian influenza H9N2 virus isolated from air samples in LPMs in Jiangxi, China |
title_sort | avian influenza h9n2 virus isolated from air samples in lpms in jiangxi, china |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5525224/ https://www.ncbi.nlm.nih.gov/pubmed/28738865 http://dx.doi.org/10.1186/s12985-017-0800-y |
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