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Hard clam extracts induce atypical apoptosis in human gastric cancer cells

Hard clams (HCs) are a nutritionally high-quality and popular seafood, and are established to be a potent antitumor food. The aim of the present study was to determine whether HC extracts induce apoptosis in the human gastric cancer cell line, AGS. In contrast with previously reported methods of ext...

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Autores principales: Song, Eing-Ju, Chan, Michael W.Y., Shin, Jyh-Wei, Chen, Che-Chun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5525584/
https://www.ncbi.nlm.nih.gov/pubmed/28810604
http://dx.doi.org/10.3892/etm.2017.4630
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author Song, Eing-Ju
Chan, Michael W.Y.
Shin, Jyh-Wei
Chen, Che-Chun
author_facet Song, Eing-Ju
Chan, Michael W.Y.
Shin, Jyh-Wei
Chen, Che-Chun
author_sort Song, Eing-Ju
collection PubMed
description Hard clams (HCs) are a nutritionally high-quality and popular seafood, and are established to be a potent antitumor food. The aim of the present study was to determine whether HC extracts induce apoptosis in the human gastric cancer cell line, AGS. In contrast with previously reported methods of extraction, crude extracts of HC were obtained by freezing and thawing and by a method free of hot water or organic solvents. The composition, quality and properties of the HC extracts were demonstrated to be stable since the extracts that were evaluated by capillary electrophoresis and HPLC analysis at different timepoints were similar. HC extracts also have an inhibitory effect against the survival of AGS cells. Treatment with HC extracts induced a marked sub-G1 DNA peak and reduced the expression of the anti-apoptotic genes BIRC5 and KPNA2. However, hallmarks of classical apoptosis such as DNA fragmentation and apoptotic body formation were not observed, indicating atypical apoptosis. Furthermore, it was revealed that HC extracts interrupted cell cycle progression in AGS cells through altered expression of six cell cycle-associated genes: CDC20, KPNA2, BIRC5, ANAPC2, CDKN1A and RB1. The present findings suggest that HC may contribute to a novel future anticancer agent.
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spelling pubmed-55255842017-08-11 Hard clam extracts induce atypical apoptosis in human gastric cancer cells Song, Eing-Ju Chan, Michael W.Y. Shin, Jyh-Wei Chen, Che-Chun Exp Ther Med Articles Hard clams (HCs) are a nutritionally high-quality and popular seafood, and are established to be a potent antitumor food. The aim of the present study was to determine whether HC extracts induce apoptosis in the human gastric cancer cell line, AGS. In contrast with previously reported methods of extraction, crude extracts of HC were obtained by freezing and thawing and by a method free of hot water or organic solvents. The composition, quality and properties of the HC extracts were demonstrated to be stable since the extracts that were evaluated by capillary electrophoresis and HPLC analysis at different timepoints were similar. HC extracts also have an inhibitory effect against the survival of AGS cells. Treatment with HC extracts induced a marked sub-G1 DNA peak and reduced the expression of the anti-apoptotic genes BIRC5 and KPNA2. However, hallmarks of classical apoptosis such as DNA fragmentation and apoptotic body formation were not observed, indicating atypical apoptosis. Furthermore, it was revealed that HC extracts interrupted cell cycle progression in AGS cells through altered expression of six cell cycle-associated genes: CDC20, KPNA2, BIRC5, ANAPC2, CDKN1A and RB1. The present findings suggest that HC may contribute to a novel future anticancer agent. D.A. Spandidos 2017-08 2017-06-20 /pmc/articles/PMC5525584/ /pubmed/28810604 http://dx.doi.org/10.3892/etm.2017.4630 Text en Copyright: © Song et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Song, Eing-Ju
Chan, Michael W.Y.
Shin, Jyh-Wei
Chen, Che-Chun
Hard clam extracts induce atypical apoptosis in human gastric cancer cells
title Hard clam extracts induce atypical apoptosis in human gastric cancer cells
title_full Hard clam extracts induce atypical apoptosis in human gastric cancer cells
title_fullStr Hard clam extracts induce atypical apoptosis in human gastric cancer cells
title_full_unstemmed Hard clam extracts induce atypical apoptosis in human gastric cancer cells
title_short Hard clam extracts induce atypical apoptosis in human gastric cancer cells
title_sort hard clam extracts induce atypical apoptosis in human gastric cancer cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5525584/
https://www.ncbi.nlm.nih.gov/pubmed/28810604
http://dx.doi.org/10.3892/etm.2017.4630
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