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miR-187 inhibits tumor growth and invasion by directly targeting MAPK12 in osteosarcoma
MicroRNAs (miRNAs/miRs) play crucial roles in cancer development and progression. The purposes of this study were to investigate the role of miR-187 in osteosarcoma and clarify the regulation of MAPK12 by miR-187. Quantitative polymerase chain reaction was used to examine miR-187 expression in osteo...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526124/ https://www.ncbi.nlm.nih.gov/pubmed/28810556 http://dx.doi.org/10.3892/etm.2017.4624 |
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author | Cui, Chengliang Shi, Xiaoyu |
author_facet | Cui, Chengliang Shi, Xiaoyu |
author_sort | Cui, Chengliang |
collection | PubMed |
description | MicroRNAs (miRNAs/miRs) play crucial roles in cancer development and progression. The purposes of this study were to investigate the role of miR-187 in osteosarcoma and clarify the regulation of MAPK12 by miR-187. Quantitative polymerase chain reaction was used to examine miR-187 expression in osteosarcoma tissues and cell lines. The clinicopathological significance of miR-187 downregulation was further analyzed. Transwell migration and invasion assays were performed. A luciferase reporter assay was conducted to confirm the target gene of miR-187, and the results were validated in cervical cancer tissues and cell lines. MiR-187 was significantly decreased in clinical tissues and osteosarcoma cell lines. The low miR-187 level was significantly correlated with stage, node metastasis, and deep stromal invasion. Upregulation of miR-187 suppressed cell migration and invasion in vitro. MAPK12 was verified as a direct target of miR-187, which was further confirmed by the inverse expression of miR-187 and MAPK12 in patients' specimens. The newly identified miR-187/MAPK12 pathway provides an insight into osteosarcoma metastasis and may represent a novel therapeutic target. |
format | Online Article Text |
id | pubmed-5526124 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-55261242017-08-11 miR-187 inhibits tumor growth and invasion by directly targeting MAPK12 in osteosarcoma Cui, Chengliang Shi, Xiaoyu Exp Ther Med Articles MicroRNAs (miRNAs/miRs) play crucial roles in cancer development and progression. The purposes of this study were to investigate the role of miR-187 in osteosarcoma and clarify the regulation of MAPK12 by miR-187. Quantitative polymerase chain reaction was used to examine miR-187 expression in osteosarcoma tissues and cell lines. The clinicopathological significance of miR-187 downregulation was further analyzed. Transwell migration and invasion assays were performed. A luciferase reporter assay was conducted to confirm the target gene of miR-187, and the results were validated in cervical cancer tissues and cell lines. MiR-187 was significantly decreased in clinical tissues and osteosarcoma cell lines. The low miR-187 level was significantly correlated with stage, node metastasis, and deep stromal invasion. Upregulation of miR-187 suppressed cell migration and invasion in vitro. MAPK12 was verified as a direct target of miR-187, which was further confirmed by the inverse expression of miR-187 and MAPK12 in patients' specimens. The newly identified miR-187/MAPK12 pathway provides an insight into osteosarcoma metastasis and may represent a novel therapeutic target. D.A. Spandidos 2017-08 2017-06-16 /pmc/articles/PMC5526124/ /pubmed/28810556 http://dx.doi.org/10.3892/etm.2017.4624 Text en Copyright: © CUI et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Cui, Chengliang Shi, Xiaoyu miR-187 inhibits tumor growth and invasion by directly targeting MAPK12 in osteosarcoma |
title | miR-187 inhibits tumor growth and invasion by directly targeting MAPK12 in osteosarcoma |
title_full | miR-187 inhibits tumor growth and invasion by directly targeting MAPK12 in osteosarcoma |
title_fullStr | miR-187 inhibits tumor growth and invasion by directly targeting MAPK12 in osteosarcoma |
title_full_unstemmed | miR-187 inhibits tumor growth and invasion by directly targeting MAPK12 in osteosarcoma |
title_short | miR-187 inhibits tumor growth and invasion by directly targeting MAPK12 in osteosarcoma |
title_sort | mir-187 inhibits tumor growth and invasion by directly targeting mapk12 in osteosarcoma |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526124/ https://www.ncbi.nlm.nih.gov/pubmed/28810556 http://dx.doi.org/10.3892/etm.2017.4624 |
work_keys_str_mv | AT cuichengliang mir187inhibitstumorgrowthandinvasionbydirectlytargetingmapk12inosteosarcoma AT shixiaoyu mir187inhibitstumorgrowthandinvasionbydirectlytargetingmapk12inosteosarcoma |