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Development of inducer-free expression plasmids based on IPTG-inducible promoters for Bacillus subtilis
BACKGROUND: Besides Escherichia coli, Bacillus subtilis is an important bacterial species for the production of recombinant proteins. Recombinant genes are inserted into shuttle expression vectors which replicate in both E. coli and in B. subtilis. The ligation products are first transformed into E....
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526301/ https://www.ncbi.nlm.nih.gov/pubmed/28743271 http://dx.doi.org/10.1186/s12934-017-0747-0 |