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Isolation and Characterization of “Terrein” an Antimicrobial and Antitumor Compound from Endophytic Fungus Aspergillus terreus (JAS-2) Associated from Achyranthus aspera Varanasi, India

The present study aimed at characterizing biological potentials of endophyte Aspergillus terreus JAS-2 isolated from Achyranthus aspera. Crude extracted from endophytic fungus JAS-2 was purified and chemically characterized by chromatographic and spectroscopic studies respectively. Spectral assignme...

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Autores principales: Goutam, Jyoti, Sharma, Gunjan, Tiwari, Vinod K., Mishra, Amrita, Kharwar, Ravindra N., Ramaraj, Vijayakumar, Koch, Biplob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526331/
https://www.ncbi.nlm.nih.gov/pubmed/28790982
http://dx.doi.org/10.3389/fmicb.2017.01334
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author Goutam, Jyoti
Sharma, Gunjan
Tiwari, Vinod K.
Mishra, Amrita
Kharwar, Ravindra N.
Ramaraj, Vijayakumar
Koch, Biplob
author_facet Goutam, Jyoti
Sharma, Gunjan
Tiwari, Vinod K.
Mishra, Amrita
Kharwar, Ravindra N.
Ramaraj, Vijayakumar
Koch, Biplob
author_sort Goutam, Jyoti
collection PubMed
description The present study aimed at characterizing biological potentials of endophyte Aspergillus terreus JAS-2 isolated from Achyranthus aspera. Crude extracted from endophytic fungus JAS-2 was purified and chemically characterized by chromatographic and spectroscopic studies respectively. Spectral assignment of NMR (nuclear magnetic resonance) data, (1)H proton and (13)C carbon analysis along with FTIR data elucidated the structure of compound as 4,5-Dihydroxy-3-(1-propenyl)-2-cyclopenten-1-one. After purification and identification a set of experiment was conducted to explore efficacy of compound. Results revealed that on accessing the antifungal activity of compound, growth diameter of tested phytopathogenic fungi was reduced to 50% at higher concentration taken (10 μgμl(−1)). Compound exhibited in-vitro bacterial cell inhibition at 20 μgml(−1) concentration along with moderate antioxidant behavior. Evaluation of anticancer activity against human lung cancer cell line (A-549) exhibited its IC(50) value to be 121.9 ± 4.821 μgml(−1). Further cell cycle phase distribution were analyzed on the basis of DNA content and evaluated by FACS (Fluorescence Activated Cell Sorting) and it was revealed that at 150 μgml(−1) of compound maximum cells were found in sub G1 phase which represents apoptotic dead cells. Terrein (4, 5-Dihydroxy-3-(1-propenyl)-2-cyclopenten-1-one) a multi-potential was isolated from endophytic fungus JAS-2, from well recognized medicinal herb A. aspera. To best of our knowledge, this is the first report of “Terrein” from endophytic derived fungus. This compound had also exhibited anticancer and antifungal activity against human lung cancer cell line A-549 and Bipolaris sorokiniana respectively which is causal organism of many plants disease. Hence endophytes are serving as alternative sources of drug molecules.
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spelling pubmed-55263312017-08-08 Isolation and Characterization of “Terrein” an Antimicrobial and Antitumor Compound from Endophytic Fungus Aspergillus terreus (JAS-2) Associated from Achyranthus aspera Varanasi, India Goutam, Jyoti Sharma, Gunjan Tiwari, Vinod K. Mishra, Amrita Kharwar, Ravindra N. Ramaraj, Vijayakumar Koch, Biplob Front Microbiol Microbiology The present study aimed at characterizing biological potentials of endophyte Aspergillus terreus JAS-2 isolated from Achyranthus aspera. Crude extracted from endophytic fungus JAS-2 was purified and chemically characterized by chromatographic and spectroscopic studies respectively. Spectral assignment of NMR (nuclear magnetic resonance) data, (1)H proton and (13)C carbon analysis along with FTIR data elucidated the structure of compound as 4,5-Dihydroxy-3-(1-propenyl)-2-cyclopenten-1-one. After purification and identification a set of experiment was conducted to explore efficacy of compound. Results revealed that on accessing the antifungal activity of compound, growth diameter of tested phytopathogenic fungi was reduced to 50% at higher concentration taken (10 μgμl(−1)). Compound exhibited in-vitro bacterial cell inhibition at 20 μgml(−1) concentration along with moderate antioxidant behavior. Evaluation of anticancer activity against human lung cancer cell line (A-549) exhibited its IC(50) value to be 121.9 ± 4.821 μgml(−1). Further cell cycle phase distribution were analyzed on the basis of DNA content and evaluated by FACS (Fluorescence Activated Cell Sorting) and it was revealed that at 150 μgml(−1) of compound maximum cells were found in sub G1 phase which represents apoptotic dead cells. Terrein (4, 5-Dihydroxy-3-(1-propenyl)-2-cyclopenten-1-one) a multi-potential was isolated from endophytic fungus JAS-2, from well recognized medicinal herb A. aspera. To best of our knowledge, this is the first report of “Terrein” from endophytic derived fungus. This compound had also exhibited anticancer and antifungal activity against human lung cancer cell line A-549 and Bipolaris sorokiniana respectively which is causal organism of many plants disease. Hence endophytes are serving as alternative sources of drug molecules. Frontiers Media S.A. 2017-07-25 /pmc/articles/PMC5526331/ /pubmed/28790982 http://dx.doi.org/10.3389/fmicb.2017.01334 Text en Copyright © 2017 Goutam, Sharma, Tiwari, Mishra, Kharwar, Ramaraj and Koch. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Goutam, Jyoti
Sharma, Gunjan
Tiwari, Vinod K.
Mishra, Amrita
Kharwar, Ravindra N.
Ramaraj, Vijayakumar
Koch, Biplob
Isolation and Characterization of “Terrein” an Antimicrobial and Antitumor Compound from Endophytic Fungus Aspergillus terreus (JAS-2) Associated from Achyranthus aspera Varanasi, India
title Isolation and Characterization of “Terrein” an Antimicrobial and Antitumor Compound from Endophytic Fungus Aspergillus terreus (JAS-2) Associated from Achyranthus aspera Varanasi, India
title_full Isolation and Characterization of “Terrein” an Antimicrobial and Antitumor Compound from Endophytic Fungus Aspergillus terreus (JAS-2) Associated from Achyranthus aspera Varanasi, India
title_fullStr Isolation and Characterization of “Terrein” an Antimicrobial and Antitumor Compound from Endophytic Fungus Aspergillus terreus (JAS-2) Associated from Achyranthus aspera Varanasi, India
title_full_unstemmed Isolation and Characterization of “Terrein” an Antimicrobial and Antitumor Compound from Endophytic Fungus Aspergillus terreus (JAS-2) Associated from Achyranthus aspera Varanasi, India
title_short Isolation and Characterization of “Terrein” an Antimicrobial and Antitumor Compound from Endophytic Fungus Aspergillus terreus (JAS-2) Associated from Achyranthus aspera Varanasi, India
title_sort isolation and characterization of “terrein” an antimicrobial and antitumor compound from endophytic fungus aspergillus terreus (jas-2) associated from achyranthus aspera varanasi, india
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526331/
https://www.ncbi.nlm.nih.gov/pubmed/28790982
http://dx.doi.org/10.3389/fmicb.2017.01334
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