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Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection
How the organization of genes on a chromosome shapes adaptation is essential for understanding evolutionary paths. Here, we investigate how adaptation to rapidly increasing levels of antibiotic depends on the chromosomal neighborhood of a drug-resistance gene inserted at different positions of the E...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526668/ https://www.ncbi.nlm.nih.gov/pubmed/28738969 http://dx.doi.org/10.7554/eLife.25100 |
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author | Steinrueck, Magdalena Guet, Călin C |
author_facet | Steinrueck, Magdalena Guet, Călin C |
author_sort | Steinrueck, Magdalena |
collection | PubMed |
description | How the organization of genes on a chromosome shapes adaptation is essential for understanding evolutionary paths. Here, we investigate how adaptation to rapidly increasing levels of antibiotic depends on the chromosomal neighborhood of a drug-resistance gene inserted at different positions of the Escherichia coli chromosome. Using a dual-fluorescence reporter that allows us to distinguish gene amplifications from other up-mutations, we track in real-time adaptive changes in expression of the drug-resistance gene. We find that the relative contribution of several mutation types differs systematically between loci due to properties of neighboring genes: essentiality, expression, orientation, termination, and presence of duplicates. These properties determine rate and fitness effects of gene amplification, deletions, and mutations compromising transcriptional termination. Thus, the adaptive potential of a gene under selection is a system-property with a complex genetic basis that is specific for each chromosomal locus, and it can be inferred from detailed functional and genomic data. DOI: http://dx.doi.org/10.7554/eLife.25100.001 |
format | Online Article Text |
id | pubmed-5526668 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-55266682017-07-26 Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection Steinrueck, Magdalena Guet, Călin C eLife Computational and Systems Biology How the organization of genes on a chromosome shapes adaptation is essential for understanding evolutionary paths. Here, we investigate how adaptation to rapidly increasing levels of antibiotic depends on the chromosomal neighborhood of a drug-resistance gene inserted at different positions of the Escherichia coli chromosome. Using a dual-fluorescence reporter that allows us to distinguish gene amplifications from other up-mutations, we track in real-time adaptive changes in expression of the drug-resistance gene. We find that the relative contribution of several mutation types differs systematically between loci due to properties of neighboring genes: essentiality, expression, orientation, termination, and presence of duplicates. These properties determine rate and fitness effects of gene amplification, deletions, and mutations compromising transcriptional termination. Thus, the adaptive potential of a gene under selection is a system-property with a complex genetic basis that is specific for each chromosomal locus, and it can be inferred from detailed functional and genomic data. DOI: http://dx.doi.org/10.7554/eLife.25100.001 eLife Sciences Publications, Ltd 2017-07-25 /pmc/articles/PMC5526668/ /pubmed/28738969 http://dx.doi.org/10.7554/eLife.25100 Text en © 2017, Steinrueck et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Computational and Systems Biology Steinrueck, Magdalena Guet, Călin C Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection |
title | Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection |
title_full | Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection |
title_fullStr | Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection |
title_full_unstemmed | Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection |
title_short | Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection |
title_sort | complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection |
topic | Computational and Systems Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526668/ https://www.ncbi.nlm.nih.gov/pubmed/28738969 http://dx.doi.org/10.7554/eLife.25100 |
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