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Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection

How the organization of genes on a chromosome shapes adaptation is essential for understanding evolutionary paths. Here, we investigate how adaptation to rapidly increasing levels of antibiotic depends on the chromosomal neighborhood of a drug-resistance gene inserted at different positions of the E...

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Detalles Bibliográficos
Autores principales: Steinrueck, Magdalena, Guet, Călin C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526668/
https://www.ncbi.nlm.nih.gov/pubmed/28738969
http://dx.doi.org/10.7554/eLife.25100
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author Steinrueck, Magdalena
Guet, Călin C
author_facet Steinrueck, Magdalena
Guet, Călin C
author_sort Steinrueck, Magdalena
collection PubMed
description How the organization of genes on a chromosome shapes adaptation is essential for understanding evolutionary paths. Here, we investigate how adaptation to rapidly increasing levels of antibiotic depends on the chromosomal neighborhood of a drug-resistance gene inserted at different positions of the Escherichia coli chromosome. Using a dual-fluorescence reporter that allows us to distinguish gene amplifications from other up-mutations, we track in real-time adaptive changes in expression of the drug-resistance gene. We find that the relative contribution of several mutation types differs systematically between loci due to properties of neighboring genes: essentiality, expression, orientation, termination, and presence of duplicates. These properties determine rate and fitness effects of gene amplification, deletions, and mutations compromising transcriptional termination. Thus, the adaptive potential of a gene under selection is a system-property with a complex genetic basis that is specific for each chromosomal locus, and it can be inferred from detailed functional and genomic data. DOI: http://dx.doi.org/10.7554/eLife.25100.001
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spelling pubmed-55266682017-07-26 Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection Steinrueck, Magdalena Guet, Călin C eLife Computational and Systems Biology How the organization of genes on a chromosome shapes adaptation is essential for understanding evolutionary paths. Here, we investigate how adaptation to rapidly increasing levels of antibiotic depends on the chromosomal neighborhood of a drug-resistance gene inserted at different positions of the Escherichia coli chromosome. Using a dual-fluorescence reporter that allows us to distinguish gene amplifications from other up-mutations, we track in real-time adaptive changes in expression of the drug-resistance gene. We find that the relative contribution of several mutation types differs systematically between loci due to properties of neighboring genes: essentiality, expression, orientation, termination, and presence of duplicates. These properties determine rate and fitness effects of gene amplification, deletions, and mutations compromising transcriptional termination. Thus, the adaptive potential of a gene under selection is a system-property with a complex genetic basis that is specific for each chromosomal locus, and it can be inferred from detailed functional and genomic data. DOI: http://dx.doi.org/10.7554/eLife.25100.001 eLife Sciences Publications, Ltd 2017-07-25 /pmc/articles/PMC5526668/ /pubmed/28738969 http://dx.doi.org/10.7554/eLife.25100 Text en © 2017, Steinrueck et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Computational and Systems Biology
Steinrueck, Magdalena
Guet, Călin C
Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection
title Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection
title_full Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection
title_fullStr Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection
title_full_unstemmed Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection
title_short Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection
title_sort complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection
topic Computational and Systems Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526668/
https://www.ncbi.nlm.nih.gov/pubmed/28738969
http://dx.doi.org/10.7554/eLife.25100
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