A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout

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We have combined a machine-learning approach with other strategies to optimize knockout efficiency with the CRISPR/Cas9 system. In addition, we have developed a multiplexed sgRNA expression strategy that promotes the functional ablation of single genes and allows for combinatorial targeting. These s...

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Detalles Bibliográficos
Autores principales: Erard, Nicolas, Knott, Simon R.V., Hannon, Gregory J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2017
Materias:
Technology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526787/
https://www.ncbi.nlm.nih.gov/pubmed/28732207
http://dx.doi.org/10.1016/j.molcel.2017.06.030
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author Erard, Nicolas
Knott, Simon R.V.
Hannon, Gregory J.
author_facet Erard, Nicolas
Knott, Simon R.V.
Hannon, Gregory J.
author_sort Erard, Nicolas
collection PubMed
description We have combined a machine-learning approach with other strategies to optimize knockout efficiency with the CRISPR/Cas9 system. In addition, we have developed a multiplexed sgRNA expression strategy that promotes the functional ablation of single genes and allows for combinatorial targeting. These strategies have been combined to design and construct a genome-wide, sequence-verified, arrayed CRISPR library. This resource allows single-target or combinatorial genetic screens to be carried out at scale in a multiplexed or arrayed format. By conducting parallel loss-of-function screens, we compare our approach to existing sgRNA design and expression strategies.
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spelling pubmed-55267872017-07-31 A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout Erard, Nicolas Knott, Simon R.V. Hannon, Gregory J. Mol Cell Technology We have combined a machine-learning approach with other strategies to optimize knockout efficiency with the CRISPR/Cas9 system. In addition, we have developed a multiplexed sgRNA expression strategy that promotes the functional ablation of single genes and allows for combinatorial targeting. These strategies have been combined to design and construct a genome-wide, sequence-verified, arrayed CRISPR library. This resource allows single-target or combinatorial genetic screens to be carried out at scale in a multiplexed or arrayed format. By conducting parallel loss-of-function screens, we compare our approach to existing sgRNA design and expression strategies. Cell Press 2017-07-20 /pmc/articles/PMC5526787/ /pubmed/28732207 http://dx.doi.org/10.1016/j.molcel.2017.06.030 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Technology
Erard, Nicolas
Knott, Simon R.V.
Hannon, Gregory J.
A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout
title A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout
title_full A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout
title_fullStr A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout
title_full_unstemmed A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout
title_short A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout
title_sort crispr resource for individual, combinatorial, or multiplexed gene knockout
topic Technology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526787/
https://www.ncbi.nlm.nih.gov/pubmed/28732207
http://dx.doi.org/10.1016/j.molcel.2017.06.030
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