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Structure of Full-Length SMC and Rearrangements Required for Chromosome Organization

Multi-subunit SMC complexes control chromosome superstructure and promote chromosome disjunction, conceivably by actively translocating along DNA double helices. SMC subunits comprise an ABC ATPase “head” and a “hinge” dimerization domain connected by a 49 nm coiled-coil “arm.” The heads undergo ATP...

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Autores principales: Diebold-Durand, Marie-Laure, Lee, Hansol, Ruiz Avila, Laura B., Noh, Haemin, Shin, Ho-Chul, Im, Haeri, Bock, Florian P., Bürmann, Frank, Durand, Alexandre, Basfeld, Alrun, Ham, Sihyun, Basquin, Jérôme, Oh, Byung-Ha, Gruber, Stephan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526789/
https://www.ncbi.nlm.nih.gov/pubmed/28689660
http://dx.doi.org/10.1016/j.molcel.2017.06.010
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author Diebold-Durand, Marie-Laure
Lee, Hansol
Ruiz Avila, Laura B.
Noh, Haemin
Shin, Ho-Chul
Im, Haeri
Bock, Florian P.
Bürmann, Frank
Durand, Alexandre
Basfeld, Alrun
Ham, Sihyun
Basquin, Jérôme
Oh, Byung-Ha
Gruber, Stephan
author_facet Diebold-Durand, Marie-Laure
Lee, Hansol
Ruiz Avila, Laura B.
Noh, Haemin
Shin, Ho-Chul
Im, Haeri
Bock, Florian P.
Bürmann, Frank
Durand, Alexandre
Basfeld, Alrun
Ham, Sihyun
Basquin, Jérôme
Oh, Byung-Ha
Gruber, Stephan
author_sort Diebold-Durand, Marie-Laure
collection PubMed
description Multi-subunit SMC complexes control chromosome superstructure and promote chromosome disjunction, conceivably by actively translocating along DNA double helices. SMC subunits comprise an ABC ATPase “head” and a “hinge” dimerization domain connected by a 49 nm coiled-coil “arm.” The heads undergo ATP-dependent engagement and disengagement to drive SMC action on the chromosome. Here, we elucidate the architecture of prokaryotic Smc dimers by high-throughput cysteine cross-linking and crystallography. Co-alignment of the Smc arms tightly closes the interarm space and misaligns the Smc head domains at the end of the rod by close apposition of their ABC signature motifs. Sandwiching of ATP molecules between Smc heads requires them to substantially tilt and translate relative to each other, thereby opening up the Smc arms. We show that this mechanochemical gating reaction regulates chromosome targeting and propose a mechanism for DNA translocation based on the merging of DNA loops upon closure of Smc arms.
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spelling pubmed-55267892017-07-31 Structure of Full-Length SMC and Rearrangements Required for Chromosome Organization Diebold-Durand, Marie-Laure Lee, Hansol Ruiz Avila, Laura B. Noh, Haemin Shin, Ho-Chul Im, Haeri Bock, Florian P. Bürmann, Frank Durand, Alexandre Basfeld, Alrun Ham, Sihyun Basquin, Jérôme Oh, Byung-Ha Gruber, Stephan Mol Cell Article Multi-subunit SMC complexes control chromosome superstructure and promote chromosome disjunction, conceivably by actively translocating along DNA double helices. SMC subunits comprise an ABC ATPase “head” and a “hinge” dimerization domain connected by a 49 nm coiled-coil “arm.” The heads undergo ATP-dependent engagement and disengagement to drive SMC action on the chromosome. Here, we elucidate the architecture of prokaryotic Smc dimers by high-throughput cysteine cross-linking and crystallography. Co-alignment of the Smc arms tightly closes the interarm space and misaligns the Smc head domains at the end of the rod by close apposition of their ABC signature motifs. Sandwiching of ATP molecules between Smc heads requires them to substantially tilt and translate relative to each other, thereby opening up the Smc arms. We show that this mechanochemical gating reaction regulates chromosome targeting and propose a mechanism for DNA translocation based on the merging of DNA loops upon closure of Smc arms. Cell Press 2017-07-20 /pmc/articles/PMC5526789/ /pubmed/28689660 http://dx.doi.org/10.1016/j.molcel.2017.06.010 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Diebold-Durand, Marie-Laure
Lee, Hansol
Ruiz Avila, Laura B.
Noh, Haemin
Shin, Ho-Chul
Im, Haeri
Bock, Florian P.
Bürmann, Frank
Durand, Alexandre
Basfeld, Alrun
Ham, Sihyun
Basquin, Jérôme
Oh, Byung-Ha
Gruber, Stephan
Structure of Full-Length SMC and Rearrangements Required for Chromosome Organization
title Structure of Full-Length SMC and Rearrangements Required for Chromosome Organization
title_full Structure of Full-Length SMC and Rearrangements Required for Chromosome Organization
title_fullStr Structure of Full-Length SMC and Rearrangements Required for Chromosome Organization
title_full_unstemmed Structure of Full-Length SMC and Rearrangements Required for Chromosome Organization
title_short Structure of Full-Length SMC and Rearrangements Required for Chromosome Organization
title_sort structure of full-length smc and rearrangements required for chromosome organization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526789/
https://www.ncbi.nlm.nih.gov/pubmed/28689660
http://dx.doi.org/10.1016/j.molcel.2017.06.010
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