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High Resolution UHPLC-MS Metabolomics and Sedative-Anxiolytic Effects of Latua pubiflora: A Mystic Plant used by Mapuche Amerindians

Latua pubiflora (Griseb) Phil. Is a native shrub of the Solanaceae family that grows freely in southern Chile and is employed among Mapuche aboriginals to induce sedative effects and hallucinations in religious or medicine rituals since prehispanic times. In this work, the pentobarbital-induced slee...

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Autores principales: Sánchez-Montoya, Eliana L., Reyes, Marco A., Pardo, Joel, Nuñez-Alarcón, Juana, Ortiz, José G., Jorge, Juan C., Bórquez, Jorge, Mocan, Andrei, Simirgiotis, Mario J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5527703/
https://www.ncbi.nlm.nih.gov/pubmed/28798689
http://dx.doi.org/10.3389/fphar.2017.00494
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author Sánchez-Montoya, Eliana L.
Reyes, Marco A.
Pardo, Joel
Nuñez-Alarcón, Juana
Ortiz, José G.
Jorge, Juan C.
Bórquez, Jorge
Mocan, Andrei
Simirgiotis, Mario J.
author_facet Sánchez-Montoya, Eliana L.
Reyes, Marco A.
Pardo, Joel
Nuñez-Alarcón, Juana
Ortiz, José G.
Jorge, Juan C.
Bórquez, Jorge
Mocan, Andrei
Simirgiotis, Mario J.
author_sort Sánchez-Montoya, Eliana L.
collection PubMed
description Latua pubiflora (Griseb) Phil. Is a native shrub of the Solanaceae family that grows freely in southern Chile and is employed among Mapuche aboriginals to induce sedative effects and hallucinations in religious or medicine rituals since prehispanic times. In this work, the pentobarbital-induced sleeping test and the elevated plus maze test were employed to test the behavioral effects of extracts of this plant in mice. The psychopharmacological evaluation of L. pubiflora extracts in mice determined that both alkaloid-enriched as well as the non-alkaloid extracts produced an increase of sleeping time and alteration of motor activity in mice at 150 mg/Kg. The alkaloid extract exhibited anxiolytic effects in the elevated plus maze test, which was counteracted by flumazenil. In addition, the alkaloid extract from L. pubiflora decreased [(3)H]-flunitrazepam binding on rat cortical membranes. In this study we have identified 18 tropane alkaloids (peaks 1–4, 8–13, 15–18, 21, 23, 24, and 28), 8 phenolic acids and related compounds (peaks 5–7, 14, 19, 20, 22, and 29) and 7 flavonoids (peaks 25–27 and 30–33) in extracts of L. pubiflora by UHPLC-PDA-MS which are responsible for the biological activity. This study assessed for the first time the sedative-anxiolytic effects of L. pubiflora in rats besides the high resolution metabolomics analysis including the finding of pharmacologically important tropane alkaloids and glycosylated flavonoids.
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spelling pubmed-55277032017-08-10 High Resolution UHPLC-MS Metabolomics and Sedative-Anxiolytic Effects of Latua pubiflora: A Mystic Plant used by Mapuche Amerindians Sánchez-Montoya, Eliana L. Reyes, Marco A. Pardo, Joel Nuñez-Alarcón, Juana Ortiz, José G. Jorge, Juan C. Bórquez, Jorge Mocan, Andrei Simirgiotis, Mario J. Front Pharmacol Pharmacology Latua pubiflora (Griseb) Phil. Is a native shrub of the Solanaceae family that grows freely in southern Chile and is employed among Mapuche aboriginals to induce sedative effects and hallucinations in religious or medicine rituals since prehispanic times. In this work, the pentobarbital-induced sleeping test and the elevated plus maze test were employed to test the behavioral effects of extracts of this plant in mice. The psychopharmacological evaluation of L. pubiflora extracts in mice determined that both alkaloid-enriched as well as the non-alkaloid extracts produced an increase of sleeping time and alteration of motor activity in mice at 150 mg/Kg. The alkaloid extract exhibited anxiolytic effects in the elevated plus maze test, which was counteracted by flumazenil. In addition, the alkaloid extract from L. pubiflora decreased [(3)H]-flunitrazepam binding on rat cortical membranes. In this study we have identified 18 tropane alkaloids (peaks 1–4, 8–13, 15–18, 21, 23, 24, and 28), 8 phenolic acids and related compounds (peaks 5–7, 14, 19, 20, 22, and 29) and 7 flavonoids (peaks 25–27 and 30–33) in extracts of L. pubiflora by UHPLC-PDA-MS which are responsible for the biological activity. This study assessed for the first time the sedative-anxiolytic effects of L. pubiflora in rats besides the high resolution metabolomics analysis including the finding of pharmacologically important tropane alkaloids and glycosylated flavonoids. Frontiers Media S.A. 2017-07-26 /pmc/articles/PMC5527703/ /pubmed/28798689 http://dx.doi.org/10.3389/fphar.2017.00494 Text en Copyright © 2017 Sánchez-Montoya, Reyes, Pardo, Nuñez-Alarcón, Ortiz, Jorge, Bórquez, Mocan and Simirgiotis. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Sánchez-Montoya, Eliana L.
Reyes, Marco A.
Pardo, Joel
Nuñez-Alarcón, Juana
Ortiz, José G.
Jorge, Juan C.
Bórquez, Jorge
Mocan, Andrei
Simirgiotis, Mario J.
High Resolution UHPLC-MS Metabolomics and Sedative-Anxiolytic Effects of Latua pubiflora: A Mystic Plant used by Mapuche Amerindians
title High Resolution UHPLC-MS Metabolomics and Sedative-Anxiolytic Effects of Latua pubiflora: A Mystic Plant used by Mapuche Amerindians
title_full High Resolution UHPLC-MS Metabolomics and Sedative-Anxiolytic Effects of Latua pubiflora: A Mystic Plant used by Mapuche Amerindians
title_fullStr High Resolution UHPLC-MS Metabolomics and Sedative-Anxiolytic Effects of Latua pubiflora: A Mystic Plant used by Mapuche Amerindians
title_full_unstemmed High Resolution UHPLC-MS Metabolomics and Sedative-Anxiolytic Effects of Latua pubiflora: A Mystic Plant used by Mapuche Amerindians
title_short High Resolution UHPLC-MS Metabolomics and Sedative-Anxiolytic Effects of Latua pubiflora: A Mystic Plant used by Mapuche Amerindians
title_sort high resolution uhplc-ms metabolomics and sedative-anxiolytic effects of latua pubiflora: a mystic plant used by mapuche amerindians
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5527703/
https://www.ncbi.nlm.nih.gov/pubmed/28798689
http://dx.doi.org/10.3389/fphar.2017.00494
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