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Microwave & magnetic proteomics of macrophages from patients with HIV-associated cognitive impairment

OBJECTIVE: HIV-infected monocytes can infiltrate the blood brain barrier as differentiated macrophages to the central nervous system, becoming the primary source of viral and cellular neurotoxins. The final outcome is HIV-associated cognitive impairment (HACI), which remain prevalent today, possibly...

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Autores principales: Cantres-Rosario, Yisel M., Acevedo-Mariani, Frances M., Pérez-Laspiur, Juliana, Haskins, William E., Plaud, Marines, Cantres-Rosario, Yadira M., Skolasky, Richard, Méndez-Bermúdez, Israel, Wojna, Valerie, Meléndez, Loyda M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5528838/
https://www.ncbi.nlm.nih.gov/pubmed/28746408
http://dx.doi.org/10.1371/journal.pone.0181779
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author Cantres-Rosario, Yisel M.
Acevedo-Mariani, Frances M.
Pérez-Laspiur, Juliana
Haskins, William E.
Plaud, Marines
Cantres-Rosario, Yadira M.
Skolasky, Richard
Méndez-Bermúdez, Israel
Wojna, Valerie
Meléndez, Loyda M.
author_facet Cantres-Rosario, Yisel M.
Acevedo-Mariani, Frances M.
Pérez-Laspiur, Juliana
Haskins, William E.
Plaud, Marines
Cantres-Rosario, Yadira M.
Skolasky, Richard
Méndez-Bermúdez, Israel
Wojna, Valerie
Meléndez, Loyda M.
author_sort Cantres-Rosario, Yisel M.
collection PubMed
description OBJECTIVE: HIV-infected monocytes can infiltrate the blood brain barrier as differentiated macrophages to the central nervous system, becoming the primary source of viral and cellular neurotoxins. The final outcome is HIV-associated cognitive impairment (HACI), which remain prevalent today, possibly due to the longer life-span of the patients treated with combined anti-retroviral therapy. Our main goal was to characterize the proteome of monocyte-derived macrophages (MDM) from HACI patients, and its association with their cognitive status, to find novel targets for therapy. METHODS: MDM were isolated from the peripheral blood of 14 HIV-seropositive women characterized for neurocognitive function, including: four normal cognition (NC), five asymptomatic (A), and five with cognitive impaired (CI). Proteins from macrophage lysates were isobaric-labeled with the microwave and magnetic (M2) sample preparation method followed by liquid chromatography-tandem mass spectrometry-based protein identification and quantification. Differences in protein abundance across groups classified by HACI status were determined using analysis of variance. RESULTS: A total of 2,519 proteins were identified with 2 or more peptides and 28 proteins were quantified as differentially expressed. Statistical analysis revealed increased abundance of 17 proteins in patients with HACI (p<0.05), including several enzymes associated to the glucose metabolism. Western blot confirmed increased expression of 6-Phosphogluconate dehydrogenase and L-Plastin in A and CI patients over NC and HIV seronegatives. CONCLUSIONS: This is the first quantitative proteomics study exploring the changes in protein abundance of macrophages isolated from patients with HACI. Further studies are warranted to determine if these proteins may be target candidates for therapy development against HACI.
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spelling pubmed-55288382017-08-07 Microwave & magnetic proteomics of macrophages from patients with HIV-associated cognitive impairment Cantres-Rosario, Yisel M. Acevedo-Mariani, Frances M. Pérez-Laspiur, Juliana Haskins, William E. Plaud, Marines Cantres-Rosario, Yadira M. Skolasky, Richard Méndez-Bermúdez, Israel Wojna, Valerie Meléndez, Loyda M. PLoS One Research Article OBJECTIVE: HIV-infected monocytes can infiltrate the blood brain barrier as differentiated macrophages to the central nervous system, becoming the primary source of viral and cellular neurotoxins. The final outcome is HIV-associated cognitive impairment (HACI), which remain prevalent today, possibly due to the longer life-span of the patients treated with combined anti-retroviral therapy. Our main goal was to characterize the proteome of monocyte-derived macrophages (MDM) from HACI patients, and its association with their cognitive status, to find novel targets for therapy. METHODS: MDM were isolated from the peripheral blood of 14 HIV-seropositive women characterized for neurocognitive function, including: four normal cognition (NC), five asymptomatic (A), and five with cognitive impaired (CI). Proteins from macrophage lysates were isobaric-labeled with the microwave and magnetic (M2) sample preparation method followed by liquid chromatography-tandem mass spectrometry-based protein identification and quantification. Differences in protein abundance across groups classified by HACI status were determined using analysis of variance. RESULTS: A total of 2,519 proteins were identified with 2 or more peptides and 28 proteins were quantified as differentially expressed. Statistical analysis revealed increased abundance of 17 proteins in patients with HACI (p<0.05), including several enzymes associated to the glucose metabolism. Western blot confirmed increased expression of 6-Phosphogluconate dehydrogenase and L-Plastin in A and CI patients over NC and HIV seronegatives. CONCLUSIONS: This is the first quantitative proteomics study exploring the changes in protein abundance of macrophages isolated from patients with HACI. Further studies are warranted to determine if these proteins may be target candidates for therapy development against HACI. Public Library of Science 2017-07-26 /pmc/articles/PMC5528838/ /pubmed/28746408 http://dx.doi.org/10.1371/journal.pone.0181779 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Cantres-Rosario, Yisel M.
Acevedo-Mariani, Frances M.
Pérez-Laspiur, Juliana
Haskins, William E.
Plaud, Marines
Cantres-Rosario, Yadira M.
Skolasky, Richard
Méndez-Bermúdez, Israel
Wojna, Valerie
Meléndez, Loyda M.
Microwave & magnetic proteomics of macrophages from patients with HIV-associated cognitive impairment
title Microwave & magnetic proteomics of macrophages from patients with HIV-associated cognitive impairment
title_full Microwave & magnetic proteomics of macrophages from patients with HIV-associated cognitive impairment
title_fullStr Microwave & magnetic proteomics of macrophages from patients with HIV-associated cognitive impairment
title_full_unstemmed Microwave & magnetic proteomics of macrophages from patients with HIV-associated cognitive impairment
title_short Microwave & magnetic proteomics of macrophages from patients with HIV-associated cognitive impairment
title_sort microwave & magnetic proteomics of macrophages from patients with hiv-associated cognitive impairment
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5528838/
https://www.ncbi.nlm.nih.gov/pubmed/28746408
http://dx.doi.org/10.1371/journal.pone.0181779
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