Cargando…
Parallel comparative studies on toxicity of quantum dots synthesized and surface engineered with different methods in vitro and in vivo
Quantum dots (QDs) have been considered to be promising probes for biosensing, bioimaging, and diagnosis. However, their toxicity issues caused by heavy metals in QDs remain to be addressed, in particular for their in vivo biomedical applications. In this study, a parallel comparative investigation...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5529378/ https://www.ncbi.nlm.nih.gov/pubmed/28790821 http://dx.doi.org/10.2147/IJN.S137637 |
_version_ | 1783253107188170752 |
---|---|
author | Liu, Fengjun Ye, Wen Wang, Jun Song, Fengxiang Cheng, Yingsheng Zhang, Bingbo |
author_facet | Liu, Fengjun Ye, Wen Wang, Jun Song, Fengxiang Cheng, Yingsheng Zhang, Bingbo |
author_sort | Liu, Fengjun |
collection | PubMed |
description | Quantum dots (QDs) have been considered to be promising probes for biosensing, bioimaging, and diagnosis. However, their toxicity issues caused by heavy metals in QDs remain to be addressed, in particular for their in vivo biomedical applications. In this study, a parallel comparative investigation in vitro and in vivo is presented to disclose the impact of synthetic methods and their following surface modifications on the toxicity of QDs. Cellular assays after exposure to QDs were conducted including cell viability assessment, DNA breakage study in a single cellular level, intracellular reactive oxygen species (ROS) receptor measurement, and transmission electron microscopy to evaluate their toxicity in vitro. Mice experiments after QD administration, including analysis of hemobiological indices, pharmacokinetics, histological examination, and body weight, were further carried out to evaluate their systematic toxicity in vivo. Results show that QDs fabricated by the thermal decomposition approach in organic phase and encapsulated by an amphiphilic polymer (denoted as QDs-1) present the least toxicity in acute damage, compared with those of QDs surface engineered by glutathione-mediated ligand exchange (denoted as QDs-2), and the ones prepared by coprecipitation approach in aqueous phase with mercaptopropionic acid capped (denoted as QDs-3). With the extension of the investigation time of mice respectively injected with QDs, we found that the damage caused by QDs to the organs can be gradually recovered. This parallel comparative investigation suggests that synthetic methods and their resulting surface microenvironment play vital roles in the acute toxicity profiles of QDs. The present study provides updated insights into the fabrication and surface engineering of QDs for their translational applications in theranostics. |
format | Online Article Text |
id | pubmed-5529378 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-55293782017-08-08 Parallel comparative studies on toxicity of quantum dots synthesized and surface engineered with different methods in vitro and in vivo Liu, Fengjun Ye, Wen Wang, Jun Song, Fengxiang Cheng, Yingsheng Zhang, Bingbo Int J Nanomedicine Original Research Quantum dots (QDs) have been considered to be promising probes for biosensing, bioimaging, and diagnosis. However, their toxicity issues caused by heavy metals in QDs remain to be addressed, in particular for their in vivo biomedical applications. In this study, a parallel comparative investigation in vitro and in vivo is presented to disclose the impact of synthetic methods and their following surface modifications on the toxicity of QDs. Cellular assays after exposure to QDs were conducted including cell viability assessment, DNA breakage study in a single cellular level, intracellular reactive oxygen species (ROS) receptor measurement, and transmission electron microscopy to evaluate their toxicity in vitro. Mice experiments after QD administration, including analysis of hemobiological indices, pharmacokinetics, histological examination, and body weight, were further carried out to evaluate their systematic toxicity in vivo. Results show that QDs fabricated by the thermal decomposition approach in organic phase and encapsulated by an amphiphilic polymer (denoted as QDs-1) present the least toxicity in acute damage, compared with those of QDs surface engineered by glutathione-mediated ligand exchange (denoted as QDs-2), and the ones prepared by coprecipitation approach in aqueous phase with mercaptopropionic acid capped (denoted as QDs-3). With the extension of the investigation time of mice respectively injected with QDs, we found that the damage caused by QDs to the organs can be gradually recovered. This parallel comparative investigation suggests that synthetic methods and their resulting surface microenvironment play vital roles in the acute toxicity profiles of QDs. The present study provides updated insights into the fabrication and surface engineering of QDs for their translational applications in theranostics. Dove Medical Press 2017-07-19 /pmc/articles/PMC5529378/ /pubmed/28790821 http://dx.doi.org/10.2147/IJN.S137637 Text en © 2017 Liu et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Liu, Fengjun Ye, Wen Wang, Jun Song, Fengxiang Cheng, Yingsheng Zhang, Bingbo Parallel comparative studies on toxicity of quantum dots synthesized and surface engineered with different methods in vitro and in vivo |
title | Parallel comparative studies on toxicity of quantum dots synthesized and surface engineered with different methods in vitro and in vivo |
title_full | Parallel comparative studies on toxicity of quantum dots synthesized and surface engineered with different methods in vitro and in vivo |
title_fullStr | Parallel comparative studies on toxicity of quantum dots synthesized and surface engineered with different methods in vitro and in vivo |
title_full_unstemmed | Parallel comparative studies on toxicity of quantum dots synthesized and surface engineered with different methods in vitro and in vivo |
title_short | Parallel comparative studies on toxicity of quantum dots synthesized and surface engineered with different methods in vitro and in vivo |
title_sort | parallel comparative studies on toxicity of quantum dots synthesized and surface engineered with different methods in vitro and in vivo |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5529378/ https://www.ncbi.nlm.nih.gov/pubmed/28790821 http://dx.doi.org/10.2147/IJN.S137637 |
work_keys_str_mv | AT liufengjun parallelcomparativestudiesontoxicityofquantumdotssynthesizedandsurfaceengineeredwithdifferentmethodsinvitroandinvivo AT yewen parallelcomparativestudiesontoxicityofquantumdotssynthesizedandsurfaceengineeredwithdifferentmethodsinvitroandinvivo AT wangjun parallelcomparativestudiesontoxicityofquantumdotssynthesizedandsurfaceengineeredwithdifferentmethodsinvitroandinvivo AT songfengxiang parallelcomparativestudiesontoxicityofquantumdotssynthesizedandsurfaceengineeredwithdifferentmethodsinvitroandinvivo AT chengyingsheng parallelcomparativestudiesontoxicityofquantumdotssynthesizedandsurfaceengineeredwithdifferentmethodsinvitroandinvivo AT zhangbingbo parallelcomparativestudiesontoxicityofquantumdotssynthesizedandsurfaceengineeredwithdifferentmethodsinvitroandinvivo |