Label-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework

A sensitive, selective and recyclable electrochemical sensor is designed for ATP detection based on amino-functionalized metal-organic framework. The functional MOF as the sensor is constructed by one-step synthesis Ce-MOF and sequentially modified on the Au electrode and conjugated with the aptamer...

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Autores principales: Shi, Pengfei, Zhang, Yuanchao, Yu, Zhaopeng, Zhang, Shusheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5529508/
https://www.ncbi.nlm.nih.gov/pubmed/28747636
http://dx.doi.org/10.1038/s41598-017-06858-w
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author Shi, Pengfei
Zhang, Yuanchao
Yu, Zhaopeng
Zhang, Shusheng
author_facet Shi, Pengfei
Zhang, Yuanchao
Yu, Zhaopeng
Zhang, Shusheng
author_sort Shi, Pengfei
collection PubMed
description A sensitive, selective and recyclable electrochemical sensor is designed for ATP detection based on amino-functionalized metal-organic framework. The functional MOF as the sensor is constructed by one-step synthesis Ce-MOF and sequentially modified on the Au electrode and conjugated with the aptamer of ATP. The presence of target ATP leads to the conformational change of aptamer strands and strong electrochemical impedance. The electrochemical sensor can detect ATP down to 5.6 nM with the linear range of 10 nm to 1000 μM. The present study is the first report on the use of MOF as an electrochemical sensor for ATP at nM level. This strategy has been successfully applied in detection of ATP in serum of cancer patients, which reveals its potential application in clinical diagnosis.
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spelling pubmed-55295082017-08-02 Label-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework Shi, Pengfei Zhang, Yuanchao Yu, Zhaopeng Zhang, Shusheng Sci Rep Article A sensitive, selective and recyclable electrochemical sensor is designed for ATP detection based on amino-functionalized metal-organic framework. The functional MOF as the sensor is constructed by one-step synthesis Ce-MOF and sequentially modified on the Au electrode and conjugated with the aptamer of ATP. The presence of target ATP leads to the conformational change of aptamer strands and strong electrochemical impedance. The electrochemical sensor can detect ATP down to 5.6 nM with the linear range of 10 nm to 1000 μM. The present study is the first report on the use of MOF as an electrochemical sensor for ATP at nM level. This strategy has been successfully applied in detection of ATP in serum of cancer patients, which reveals its potential application in clinical diagnosis. Nature Publishing Group UK 2017-07-26 /pmc/articles/PMC5529508/ /pubmed/28747636 http://dx.doi.org/10.1038/s41598-017-06858-w Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Shi, Pengfei
Zhang, Yuanchao
Yu, Zhaopeng
Zhang, Shusheng
Label-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework
title Label-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework
title_full Label-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework
title_fullStr Label-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework
title_full_unstemmed Label-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework
title_short Label-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework
title_sort label-free electrochemical detection of atp based on amino-functionalized metal-organic framework
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5529508/
https://www.ncbi.nlm.nih.gov/pubmed/28747636
http://dx.doi.org/10.1038/s41598-017-06858-w
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AT zhangshusheng labelfreeelectrochemicaldetectionofatpbasedonaminofunctionalizedmetalorganicframework

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