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Engineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting

Designing biologically inspired nanoscale molecular assembly with desired functionality is a challenging endeavour. Here we report the designing of fibrin-inspired nanostructured peptide based sealants which facilitate remarkably fast entrapping of blood corpuscles (~28 seconds) in contrast to fibri...

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Autores principales: Ghosh, Snehasish, Mukherjee, Sanchita, Dutta, Chiranjit, Chakraborty, Kasturee, Gayen, Paramita, Jan, Somnath, Bhattacharyya, Dhananjay, Roy, Rituparna Sinha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5529531/
https://www.ncbi.nlm.nih.gov/pubmed/28747673
http://dx.doi.org/10.1038/s41598-017-06360-3
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author Ghosh, Snehasish
Mukherjee, Sanchita
Dutta, Chiranjit
Chakraborty, Kasturee
Gayen, Paramita
Jan, Somnath
Bhattacharyya, Dhananjay
Roy, Rituparna Sinha
author_facet Ghosh, Snehasish
Mukherjee, Sanchita
Dutta, Chiranjit
Chakraborty, Kasturee
Gayen, Paramita
Jan, Somnath
Bhattacharyya, Dhananjay
Roy, Rituparna Sinha
author_sort Ghosh, Snehasish
collection PubMed
description Designing biologically inspired nanoscale molecular assembly with desired functionality is a challenging endeavour. Here we report the designing of fibrin-inspired nanostructured peptide based sealants which facilitate remarkably fast entrapping of blood corpuscles (~28 seconds) in contrast to fibrin (~56 seconds). Our engineered sealants are stabilized by lysine-aspartate ionic interactions and also by N(ε)(γ-glutamyl) lysine isopeptide bond mediated covalent interaction. Each sealant is formed by two peptides having complementary charges to promote lysine-aspartate ionic interactions and designed isopeptide bond mediated interactions. Computational analysis reveals the isopeptide bond mediated energetically favourable peptide assemblies in sealants 1–3. Our designed sealants 2 and 3 mimic fibrin-mediated clot formation mechanism in presence of transglutaminase enzyme and blood corpuscles. These fibrin-inspired peptides assemble to form sealants having superior hemostatic activities than fibrin. Designed sealants feature mechanical properties, biocompatibility, biodegradability and high adhesive strength. Such nature-inspired robust sealants might be potentially translated into clinics for facilitating efficient blood clotting to handle traumatic coagulopathy and impaired blood clotting.
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spelling pubmed-55295312017-08-02 Engineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting Ghosh, Snehasish Mukherjee, Sanchita Dutta, Chiranjit Chakraborty, Kasturee Gayen, Paramita Jan, Somnath Bhattacharyya, Dhananjay Roy, Rituparna Sinha Sci Rep Article Designing biologically inspired nanoscale molecular assembly with desired functionality is a challenging endeavour. Here we report the designing of fibrin-inspired nanostructured peptide based sealants which facilitate remarkably fast entrapping of blood corpuscles (~28 seconds) in contrast to fibrin (~56 seconds). Our engineered sealants are stabilized by lysine-aspartate ionic interactions and also by N(ε)(γ-glutamyl) lysine isopeptide bond mediated covalent interaction. Each sealant is formed by two peptides having complementary charges to promote lysine-aspartate ionic interactions and designed isopeptide bond mediated interactions. Computational analysis reveals the isopeptide bond mediated energetically favourable peptide assemblies in sealants 1–3. Our designed sealants 2 and 3 mimic fibrin-mediated clot formation mechanism in presence of transglutaminase enzyme and blood corpuscles. These fibrin-inspired peptides assemble to form sealants having superior hemostatic activities than fibrin. Designed sealants feature mechanical properties, biocompatibility, biodegradability and high adhesive strength. Such nature-inspired robust sealants might be potentially translated into clinics for facilitating efficient blood clotting to handle traumatic coagulopathy and impaired blood clotting. Nature Publishing Group UK 2017-07-26 /pmc/articles/PMC5529531/ /pubmed/28747673 http://dx.doi.org/10.1038/s41598-017-06360-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ghosh, Snehasish
Mukherjee, Sanchita
Dutta, Chiranjit
Chakraborty, Kasturee
Gayen, Paramita
Jan, Somnath
Bhattacharyya, Dhananjay
Roy, Rituparna Sinha
Engineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting
title Engineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting
title_full Engineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting
title_fullStr Engineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting
title_full_unstemmed Engineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting
title_short Engineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting
title_sort engineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5529531/
https://www.ncbi.nlm.nih.gov/pubmed/28747673
http://dx.doi.org/10.1038/s41598-017-06360-3
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