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Morita-Baylis-Hillman Adducts Display Anti-Inflammatory Effects by Modulating Inflammatory Mediator Expression in RAW264.7 Cells

Inflammatory response plays an important role not only in the normal physiology but also in pathologies such as cancers. The Morita-Baylis-Hillman adducts (MBHA) are a novel group of synthetic molecules that have demonstrated many biological activities against some parasitic cells such as Plasmodium...

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Autores principales: Faheina-Martins, Glaucia V., Leite, Jacqueline Alves, Dantas, Bruna Braga, Lima-Júnior, Cláudio G., Vasconcellos, Mário L. A. A., Rodrigues-Mascarenhas, Sandra, Araújo, Demetrius A. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5529643/
https://www.ncbi.nlm.nih.gov/pubmed/28785138
http://dx.doi.org/10.1155/2017/6898505
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author Faheina-Martins, Glaucia V.
Leite, Jacqueline Alves
Dantas, Bruna Braga
Lima-Júnior, Cláudio G.
Vasconcellos, Mário L. A. A.
Rodrigues-Mascarenhas, Sandra
Araújo, Demetrius A. M.
author_facet Faheina-Martins, Glaucia V.
Leite, Jacqueline Alves
Dantas, Bruna Braga
Lima-Júnior, Cláudio G.
Vasconcellos, Mário L. A. A.
Rodrigues-Mascarenhas, Sandra
Araújo, Demetrius A. M.
author_sort Faheina-Martins, Glaucia V.
collection PubMed
description Inflammatory response plays an important role not only in the normal physiology but also in pathologies such as cancers. The Morita-Baylis-Hillman adducts (MBHA) are a novel group of synthetic molecules that have demonstrated many biological activities against some parasitic cells such as Plasmodium falciparum, Leishmania amazonensis, and Leishmania chagasi, and antimitotic activity against sea urchin embryonic cells was also related. However, little is known about the mechanisms induced by MBHA in inflammatory process and its relation with anticancer activity. The present work investigated the cytotoxicity of three MBHA derivatives (A2CN, A3CN, and A4CN), on human colorectal adenocarcinoma, HT-29 cells, and their anti-inflammatory activities were examined in lipopolysaccharide- (LPS-) stimulated RAW264.7 macrophage cells, being these derivatives potentially cytotoxic to HT-29 cells. Coincubation with A2CN, A3CN, or A4CN and LPS in RAW264.7 cells inhibited NO production, as well as the production of reactive oxygen species (ROS) was also repressed. The mRNA expressions of IL-1β and IL-6 were significantly downregulated by such MBHA compounds in RAW264.7 cells, but only A2CN was able to inhibit the COX-2 gene expression. We also showed that MBHA compounds decreased almost to zero the production of IL-1β and IL-6. These findings display that such MBHA compounds exhibit anticancer and anti-inflammatory activities.
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spelling pubmed-55296432017-08-07 Morita-Baylis-Hillman Adducts Display Anti-Inflammatory Effects by Modulating Inflammatory Mediator Expression in RAW264.7 Cells Faheina-Martins, Glaucia V. Leite, Jacqueline Alves Dantas, Bruna Braga Lima-Júnior, Cláudio G. Vasconcellos, Mário L. A. A. Rodrigues-Mascarenhas, Sandra Araújo, Demetrius A. M. Mediators Inflamm Research Article Inflammatory response plays an important role not only in the normal physiology but also in pathologies such as cancers. The Morita-Baylis-Hillman adducts (MBHA) are a novel group of synthetic molecules that have demonstrated many biological activities against some parasitic cells such as Plasmodium falciparum, Leishmania amazonensis, and Leishmania chagasi, and antimitotic activity against sea urchin embryonic cells was also related. However, little is known about the mechanisms induced by MBHA in inflammatory process and its relation with anticancer activity. The present work investigated the cytotoxicity of three MBHA derivatives (A2CN, A3CN, and A4CN), on human colorectal adenocarcinoma, HT-29 cells, and their anti-inflammatory activities were examined in lipopolysaccharide- (LPS-) stimulated RAW264.7 macrophage cells, being these derivatives potentially cytotoxic to HT-29 cells. Coincubation with A2CN, A3CN, or A4CN and LPS in RAW264.7 cells inhibited NO production, as well as the production of reactive oxygen species (ROS) was also repressed. The mRNA expressions of IL-1β and IL-6 were significantly downregulated by such MBHA compounds in RAW264.7 cells, but only A2CN was able to inhibit the COX-2 gene expression. We also showed that MBHA compounds decreased almost to zero the production of IL-1β and IL-6. These findings display that such MBHA compounds exhibit anticancer and anti-inflammatory activities. Hindawi 2017 2017-07-12 /pmc/articles/PMC5529643/ /pubmed/28785138 http://dx.doi.org/10.1155/2017/6898505 Text en Copyright © 2017 Glaucia V. Faheina-Martins et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Faheina-Martins, Glaucia V.
Leite, Jacqueline Alves
Dantas, Bruna Braga
Lima-Júnior, Cláudio G.
Vasconcellos, Mário L. A. A.
Rodrigues-Mascarenhas, Sandra
Araújo, Demetrius A. M.
Morita-Baylis-Hillman Adducts Display Anti-Inflammatory Effects by Modulating Inflammatory Mediator Expression in RAW264.7 Cells
title Morita-Baylis-Hillman Adducts Display Anti-Inflammatory Effects by Modulating Inflammatory Mediator Expression in RAW264.7 Cells
title_full Morita-Baylis-Hillman Adducts Display Anti-Inflammatory Effects by Modulating Inflammatory Mediator Expression in RAW264.7 Cells
title_fullStr Morita-Baylis-Hillman Adducts Display Anti-Inflammatory Effects by Modulating Inflammatory Mediator Expression in RAW264.7 Cells
title_full_unstemmed Morita-Baylis-Hillman Adducts Display Anti-Inflammatory Effects by Modulating Inflammatory Mediator Expression in RAW264.7 Cells
title_short Morita-Baylis-Hillman Adducts Display Anti-Inflammatory Effects by Modulating Inflammatory Mediator Expression in RAW264.7 Cells
title_sort morita-baylis-hillman adducts display anti-inflammatory effects by modulating inflammatory mediator expression in raw264.7 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5529643/
https://www.ncbi.nlm.nih.gov/pubmed/28785138
http://dx.doi.org/10.1155/2017/6898505
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