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Tumour-associated and non-tumour-associated microbiota in colorectal cancer

OBJECTIVE: A signature that unifies the colorectal cancer (CRC) microbiota across multiple studies has not been identified. In addition to methodological variance, heterogeneity may be caused by both microbial and host response differences, which was addressed in this study. DESIGN: We prospectively...

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Autores principales: Flemer, Burkhardt, Lynch, Denise B, Brown, Jillian M R, Jeffery, Ian B, Ryan, Feargal J, Claesson, Marcus J, O'Riordain, Micheal, Shanahan, Fergus, O'Toole, Paul W
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5529966/
https://www.ncbi.nlm.nih.gov/pubmed/26992426
http://dx.doi.org/10.1136/gutjnl-2015-309595
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author Flemer, Burkhardt
Lynch, Denise B
Brown, Jillian M R
Jeffery, Ian B
Ryan, Feargal J
Claesson, Marcus J
O'Riordain, Micheal
Shanahan, Fergus
O'Toole, Paul W
author_facet Flemer, Burkhardt
Lynch, Denise B
Brown, Jillian M R
Jeffery, Ian B
Ryan, Feargal J
Claesson, Marcus J
O'Riordain, Micheal
Shanahan, Fergus
O'Toole, Paul W
author_sort Flemer, Burkhardt
collection PubMed
description OBJECTIVE: A signature that unifies the colorectal cancer (CRC) microbiota across multiple studies has not been identified. In addition to methodological variance, heterogeneity may be caused by both microbial and host response differences, which was addressed in this study. DESIGN: We prospectively studied the colonic microbiota and the expression of specific host response genes using faecal and mucosal samples (‘ON’ and ‘OFF’ the tumour, proximal and distal) from 59 patients undergoing surgery for CRC, 21 individuals with polyps and 56 healthy controls. Microbiota composition was determined by 16S rRNA amplicon sequencing; expression of host genes involved in CRC progression and immune response was quantified by real-time quantitative PCR. RESULTS: The microbiota of patients with CRC differed from that of controls, but alterations were not restricted to the cancerous tissue. Differences between distal and proximal cancers were detected and faecal microbiota only partially reflected mucosal microbiota in CRC. Patients with CRC can be stratified based on higher level structures of mucosal-associated bacterial co-abundance groups (CAGs) that resemble the previously formulated concept of enterotypes. Of these, Bacteroidetes Cluster 1 and Firmicutes Cluster 1 were in decreased abundance in CRC mucosa, whereas Bacteroidetes Cluster 2, Firmicutes Cluster 2, Pathogen Cluster and Prevotella Cluster showed increased abundance in CRC mucosa. CRC-associated CAGs were differentially correlated with the expression of host immunoinflammatory response genes. CONCLUSIONS: CRC-associated microbiota profiles differ from those in healthy subjects and are linked with distinct mucosal gene-expression profiles. Compositional alterations in the microbiota are not restricted to cancerous tissue and differ between distal and proximal cancers.
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spelling pubmed-55299662017-07-31 Tumour-associated and non-tumour-associated microbiota in colorectal cancer Flemer, Burkhardt Lynch, Denise B Brown, Jillian M R Jeffery, Ian B Ryan, Feargal J Claesson, Marcus J O'Riordain, Micheal Shanahan, Fergus O'Toole, Paul W Gut Gut Microbiota OBJECTIVE: A signature that unifies the colorectal cancer (CRC) microbiota across multiple studies has not been identified. In addition to methodological variance, heterogeneity may be caused by both microbial and host response differences, which was addressed in this study. DESIGN: We prospectively studied the colonic microbiota and the expression of specific host response genes using faecal and mucosal samples (‘ON’ and ‘OFF’ the tumour, proximal and distal) from 59 patients undergoing surgery for CRC, 21 individuals with polyps and 56 healthy controls. Microbiota composition was determined by 16S rRNA amplicon sequencing; expression of host genes involved in CRC progression and immune response was quantified by real-time quantitative PCR. RESULTS: The microbiota of patients with CRC differed from that of controls, but alterations were not restricted to the cancerous tissue. Differences between distal and proximal cancers were detected and faecal microbiota only partially reflected mucosal microbiota in CRC. Patients with CRC can be stratified based on higher level structures of mucosal-associated bacterial co-abundance groups (CAGs) that resemble the previously formulated concept of enterotypes. Of these, Bacteroidetes Cluster 1 and Firmicutes Cluster 1 were in decreased abundance in CRC mucosa, whereas Bacteroidetes Cluster 2, Firmicutes Cluster 2, Pathogen Cluster and Prevotella Cluster showed increased abundance in CRC mucosa. CRC-associated CAGs were differentially correlated with the expression of host immunoinflammatory response genes. CONCLUSIONS: CRC-associated microbiota profiles differ from those in healthy subjects and are linked with distinct mucosal gene-expression profiles. Compositional alterations in the microbiota are not restricted to cancerous tissue and differ between distal and proximal cancers. BMJ Publishing Group 2017-04 2016-03-18 /pmc/articles/PMC5529966/ /pubmed/26992426 http://dx.doi.org/10.1136/gutjnl-2015-309595 Text en Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/ This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
spellingShingle Gut Microbiota
Flemer, Burkhardt
Lynch, Denise B
Brown, Jillian M R
Jeffery, Ian B
Ryan, Feargal J
Claesson, Marcus J
O'Riordain, Micheal
Shanahan, Fergus
O'Toole, Paul W
Tumour-associated and non-tumour-associated microbiota in colorectal cancer
title Tumour-associated and non-tumour-associated microbiota in colorectal cancer
title_full Tumour-associated and non-tumour-associated microbiota in colorectal cancer
title_fullStr Tumour-associated and non-tumour-associated microbiota in colorectal cancer
title_full_unstemmed Tumour-associated and non-tumour-associated microbiota in colorectal cancer
title_short Tumour-associated and non-tumour-associated microbiota in colorectal cancer
title_sort tumour-associated and non-tumour-associated microbiota in colorectal cancer
topic Gut Microbiota
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5529966/
https://www.ncbi.nlm.nih.gov/pubmed/26992426
http://dx.doi.org/10.1136/gutjnl-2015-309595
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