Oleic acid induces down-regulation of the granulosa cell identity marker FOXL2, and up-regulation of the Sertoli cell marker SOX9 in bovine granulosa cells

During negative energy balance, the concentration of different fatty acids, especially of oleic acid (OA) increases in the follicular fluid of cattle. Previously, we showed that OA induced morphological, physiological and molecular changes in cultured bovine granulosa cells. In our present study we...

Descripción completa

Detalles Bibliográficos
Autores principales: Yenuganti, Vengala Rao, Vanselow, Jens
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5530537/
https://www.ncbi.nlm.nih.gov/pubmed/28747195
http://dx.doi.org/10.1186/s12958-017-0276-z
_version_ 1783253283120349184
author Yenuganti, Vengala Rao
Vanselow, Jens
author_facet Yenuganti, Vengala Rao
Vanselow, Jens
author_sort Yenuganti, Vengala Rao
collection PubMed
description During negative energy balance, the concentration of different fatty acids, especially of oleic acid (OA) increases in the follicular fluid of cattle. Previously, we showed that OA induced morphological, physiological and molecular changes in cultured bovine granulosa cells. In our present study we analyzed effects of OA on the expression of markers for granulosa and Sertoli cell identity, FOXL2 and SOX9, respectively, in addition to effects on the FOXL2 regulated genes ESR2, FST, PTGS2 and PPARG. The results showed that OA down-regulated FOXL2, ESR2, FST and PPARG but up-regulated PTGS2 and SOX9. From these data we conclude that OA can compromise granulosa cell functionality and may initiate trans-differentiation processes in bovine granulosa cells. This novel mechanism may be causally involved in postpartum fertility problems of lactating dairy cows.
format Online
Article
Text
id pubmed-5530537
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-55305372017-08-02 Oleic acid induces down-regulation of the granulosa cell identity marker FOXL2, and up-regulation of the Sertoli cell marker SOX9 in bovine granulosa cells Yenuganti, Vengala Rao Vanselow, Jens Reprod Biol Endocrinol Short Communication During negative energy balance, the concentration of different fatty acids, especially of oleic acid (OA) increases in the follicular fluid of cattle. Previously, we showed that OA induced morphological, physiological and molecular changes in cultured bovine granulosa cells. In our present study we analyzed effects of OA on the expression of markers for granulosa and Sertoli cell identity, FOXL2 and SOX9, respectively, in addition to effects on the FOXL2 regulated genes ESR2, FST, PTGS2 and PPARG. The results showed that OA down-regulated FOXL2, ESR2, FST and PPARG but up-regulated PTGS2 and SOX9. From these data we conclude that OA can compromise granulosa cell functionality and may initiate trans-differentiation processes in bovine granulosa cells. This novel mechanism may be causally involved in postpartum fertility problems of lactating dairy cows. BioMed Central 2017-07-26 /pmc/articles/PMC5530537/ /pubmed/28747195 http://dx.doi.org/10.1186/s12958-017-0276-z Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Communication
Yenuganti, Vengala Rao
Vanselow, Jens
Oleic acid induces down-regulation of the granulosa cell identity marker FOXL2, and up-regulation of the Sertoli cell marker SOX9 in bovine granulosa cells
title Oleic acid induces down-regulation of the granulosa cell identity marker FOXL2, and up-regulation of the Sertoli cell marker SOX9 in bovine granulosa cells
title_full Oleic acid induces down-regulation of the granulosa cell identity marker FOXL2, and up-regulation of the Sertoli cell marker SOX9 in bovine granulosa cells
title_fullStr Oleic acid induces down-regulation of the granulosa cell identity marker FOXL2, and up-regulation of the Sertoli cell marker SOX9 in bovine granulosa cells
title_full_unstemmed Oleic acid induces down-regulation of the granulosa cell identity marker FOXL2, and up-regulation of the Sertoli cell marker SOX9 in bovine granulosa cells
title_short Oleic acid induces down-regulation of the granulosa cell identity marker FOXL2, and up-regulation of the Sertoli cell marker SOX9 in bovine granulosa cells
title_sort oleic acid induces down-regulation of the granulosa cell identity marker foxl2, and up-regulation of the sertoli cell marker sox9 in bovine granulosa cells
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5530537/
https://www.ncbi.nlm.nih.gov/pubmed/28747195
http://dx.doi.org/10.1186/s12958-017-0276-z
work_keys_str_mv AT yenugantivengalarao oleicacidinducesdownregulationofthegranulosacellidentitymarkerfoxl2andupregulationofthesertolicellmarkersox9inbovinegranulosacells
AT vanselowjens oleicacidinducesdownregulationofthegranulosacellidentitymarkerfoxl2andupregulationofthesertolicellmarkersox9inbovinegranulosacells

Ejemplares similares