Complete coding sequence of dengue virus serotype 4 isolated from field-caught mosquitoes in Thailand

This report is the first to characterise the complete coding sequence of a dengue virus serotype 4 (DENV-4) genotype I that was isolated from field-caught mosquitoes from an endemic area in Thailand in June 2013. The sequence was assembled from high-throughput sequencing reads generated by Illumina...

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Autores principales: Sittivicharpinyo, Thikhumporn, Wonnapinij, Passorn, Surat, Wunrada
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2017
Materias:
Genome Announcement and Highlights
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5530551/
https://www.ncbi.nlm.nih.gov/pubmed/28767984
http://dx.doi.org/10.1590/0074-02760170022
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author Sittivicharpinyo, Thikhumporn
Wonnapinij, Passorn
Surat, Wunrada
author_facet Sittivicharpinyo, Thikhumporn
Wonnapinij, Passorn
Surat, Wunrada
author_sort Sittivicharpinyo, Thikhumporn
collection PubMed
description This report is the first to characterise the complete coding sequence of a dengue virus serotype 4 (DENV-4) genotype I that was isolated from field-caught mosquitoes from an endemic area in Thailand in June 2013. The sequence was assembled from high-throughput sequencing reads generated by Illumina HiSeq. Three out of four observed intra-sample variants caused an amino acid variation in C, NS2B, and NS5 genes. The C4279T variant located in the NS2B gene can indirectly affect the proteolytic activity of the NS3 protein. The sequence provided in this study might be useful for the epidemiological study of DENV-4.
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spelling pubmed-55305512017-08-02 Complete coding sequence of dengue virus serotype 4 isolated from field-caught mosquitoes in Thailand Sittivicharpinyo, Thikhumporn Wonnapinij, Passorn Surat, Wunrada Mem Inst Oswaldo Cruz Genome Announcement and Highlights This report is the first to characterise the complete coding sequence of a dengue virus serotype 4 (DENV-4) genotype I that was isolated from field-caught mosquitoes from an endemic area in Thailand in June 2013. The sequence was assembled from high-throughput sequencing reads generated by Illumina HiSeq. Three out of four observed intra-sample variants caused an amino acid variation in C, NS2B, and NS5 genes. The C4279T variant located in the NS2B gene can indirectly affect the proteolytic activity of the NS3 protein. The sequence provided in this study might be useful for the epidemiological study of DENV-4. Instituto Oswaldo Cruz, Ministério da Saúde 2017-08 /pmc/articles/PMC5530551/ /pubmed/28767984 http://dx.doi.org/10.1590/0074-02760170022 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Genome Announcement and Highlights
Sittivicharpinyo, Thikhumporn
Wonnapinij, Passorn
Surat, Wunrada
Complete coding sequence of dengue virus serotype 4 isolated from field-caught mosquitoes in Thailand
title Complete coding sequence of dengue virus serotype 4 isolated from field-caught mosquitoes in Thailand
title_full Complete coding sequence of dengue virus serotype 4 isolated from field-caught mosquitoes in Thailand
title_fullStr Complete coding sequence of dengue virus serotype 4 isolated from field-caught mosquitoes in Thailand
title_full_unstemmed Complete coding sequence of dengue virus serotype 4 isolated from field-caught mosquitoes in Thailand
title_short Complete coding sequence of dengue virus serotype 4 isolated from field-caught mosquitoes in Thailand
title_sort complete coding sequence of dengue virus serotype 4 isolated from field-caught mosquitoes in thailand
topic Genome Announcement and Highlights
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5530551/
https://www.ncbi.nlm.nih.gov/pubmed/28767984
http://dx.doi.org/10.1590/0074-02760170022
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