Mass spectrometry captures structural intermediates in protein fiber self-assembly

Self-assembling proteins, the basis for a broad range of biological scaffolds, are challenging to study using most structural biology approaches. Here we show that mass spectrometry (MS) in combination with MD simulations captures structural features of short-lived oligomeric intermediates in spider...

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Detalles Bibliográficos
Autores principales: Landreh, Michael, Andersson, Marlene, Marklund, Erik G., Jia, Qiupin, Meng, Qing, Johansson, Jan, Robinson, Carol V., Rising, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2017
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5530726/
https://www.ncbi.nlm.nih.gov/pubmed/28184384
http://dx.doi.org/10.1039/c7cc00307b
Descripción
Sumario:Self-assembling proteins, the basis for a broad range of biological scaffolds, are challenging to study using most structural biology approaches. Here we show that mass spectrometry (MS) in combination with MD simulations captures structural features of short-lived oligomeric intermediates in spider silk formation, providing direct insights into its complex assembly process.

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