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High-quality frozen extracts of Xenopus laevis eggs reveal size-dependent control of metaphase spindle micromechanics
Cell-free extracts from unfertilized Xenopus laevis eggs offer the opportunity for a variety of biochemical and biophysical assays for analyzing essential cell cycle events such as metaphase spindle assembly. However, the extracts often exhibit substantial variation in quality and have low storage s...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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The American Society for Cell Biology
2017
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5531733/ https://www.ncbi.nlm.nih.gov/pubmed/28592634 http://dx.doi.org/10.1091/mbc.E17-03-0174 |