Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine

In this study, by combining nuclear magnetic resonance (NMR), circular dichroism (CD), liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS), and gel electrophoresis, we report an unusual topological structure of the RNA G-quadruplex motif formed by human telomere RNA r(UAGGGU)...

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Autores principales: Xiao, Chao-Da, Ishizuka, Takumi, Xu, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5532209/
https://www.ncbi.nlm.nih.gov/pubmed/28751647
http://dx.doi.org/10.1038/s41598-017-07050-w
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author Xiao, Chao-Da
Ishizuka, Takumi
Xu, Yan
author_facet Xiao, Chao-Da
Ishizuka, Takumi
Xu, Yan
author_sort Xiao, Chao-Da
collection PubMed
description In this study, by combining nuclear magnetic resonance (NMR), circular dichroism (CD), liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS), and gel electrophoresis, we report an unusual topological structure of the RNA G-quadruplex motif formed by human telomere RNA r(UAGGGU) containing 8-bromoguanosine. Results showed that the RNA sequence formed an antiparallel tetramolecular G-quadruplex, in which each pair of diagonal strands run in opposite directions. Furthermore, guanosines were observed both in syn- and anti-conformations. In addition, two of these G-quadruplex subunits were found to be stacking on top of each other, forming a dimeric RNA G-quadruplex. Our findings provide a new insight into the behavior of RNA G-quadruplex structures.
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spelling pubmed-55322092017-08-02 Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine Xiao, Chao-Da Ishizuka, Takumi Xu, Yan Sci Rep Article In this study, by combining nuclear magnetic resonance (NMR), circular dichroism (CD), liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS), and gel electrophoresis, we report an unusual topological structure of the RNA G-quadruplex motif formed by human telomere RNA r(UAGGGU) containing 8-bromoguanosine. Results showed that the RNA sequence formed an antiparallel tetramolecular G-quadruplex, in which each pair of diagonal strands run in opposite directions. Furthermore, guanosines were observed both in syn- and anti-conformations. In addition, two of these G-quadruplex subunits were found to be stacking on top of each other, forming a dimeric RNA G-quadruplex. Our findings provide a new insight into the behavior of RNA G-quadruplex structures. Nature Publishing Group UK 2017-07-27 /pmc/articles/PMC5532209/ /pubmed/28751647 http://dx.doi.org/10.1038/s41598-017-07050-w Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Xiao, Chao-Da
Ishizuka, Takumi
Xu, Yan
Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine
title Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine
title_full Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine
title_fullStr Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine
title_full_unstemmed Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine
title_short Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine
title_sort antiparallel rna g-quadruplex formed by human telomere rna containing 8-bromoguanosine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5532209/
https://www.ncbi.nlm.nih.gov/pubmed/28751647
http://dx.doi.org/10.1038/s41598-017-07050-w
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AT xuyan antiparallelrnagquadruplexformedbyhumantelomerernacontaining8bromoguanosine

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