Cargando…
Microfluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture
Continuous production of biologics, a growing trend in the biopharmaceutical industry, requires a reliable and efficient cell retention device that also maintains cell viability. Current filtration methods, such as tangential flow filtration using hollow-fiber membranes, suffer from membrane fouling...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5532224/ https://www.ncbi.nlm.nih.gov/pubmed/28751635 http://dx.doi.org/10.1038/s41598-017-06949-8 |
_version_ | 1783253410423767040 |
---|---|
author | Kwon, Taehong Prentice, Holly Oliveira, Jonas De Madziva, Nyasha Warkiani, Majid Ebrahimi Hamel, Jean-François P. Han, Jongyoon |
author_facet | Kwon, Taehong Prentice, Holly Oliveira, Jonas De Madziva, Nyasha Warkiani, Majid Ebrahimi Hamel, Jean-François P. Han, Jongyoon |
author_sort | Kwon, Taehong |
collection | PubMed |
description | Continuous production of biologics, a growing trend in the biopharmaceutical industry, requires a reliable and efficient cell retention device that also maintains cell viability. Current filtration methods, such as tangential flow filtration using hollow-fiber membranes, suffer from membrane fouling, leading to significant reliability and productivity issues such as low cell viability, product retention, and an increased contamination risk associated with filter replacement. We introduce a novel cell retention device based on inertial sorting for perfusion culture of suspended mammalian cells. The device was characterized in terms of cell retention capacity, biocompatibility, scalability, and long-term reliability. This technology was demonstrated using a high concentration (>20 million cells/mL) perfusion culture of an IgG(1)-producing Chinese hamster ovary (CHO) cell line for 18–25 days. The device demonstrated reliable and clog-free cell retention, high IgG(1) recovery (>99%) and cell viability (>97%). Lab-scale perfusion cultures (350 mL) were used to demonstrate the technology, which can be scaled-out with parallel devices to enable larger scale operation. The new cell retention device is thus ideal for rapid perfusion process development in a biomanufacturing workflow. |
format | Online Article Text |
id | pubmed-5532224 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55322242017-08-02 Microfluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture Kwon, Taehong Prentice, Holly Oliveira, Jonas De Madziva, Nyasha Warkiani, Majid Ebrahimi Hamel, Jean-François P. Han, Jongyoon Sci Rep Article Continuous production of biologics, a growing trend in the biopharmaceutical industry, requires a reliable and efficient cell retention device that also maintains cell viability. Current filtration methods, such as tangential flow filtration using hollow-fiber membranes, suffer from membrane fouling, leading to significant reliability and productivity issues such as low cell viability, product retention, and an increased contamination risk associated with filter replacement. We introduce a novel cell retention device based on inertial sorting for perfusion culture of suspended mammalian cells. The device was characterized in terms of cell retention capacity, biocompatibility, scalability, and long-term reliability. This technology was demonstrated using a high concentration (>20 million cells/mL) perfusion culture of an IgG(1)-producing Chinese hamster ovary (CHO) cell line for 18–25 days. The device demonstrated reliable and clog-free cell retention, high IgG(1) recovery (>99%) and cell viability (>97%). Lab-scale perfusion cultures (350 mL) were used to demonstrate the technology, which can be scaled-out with parallel devices to enable larger scale operation. The new cell retention device is thus ideal for rapid perfusion process development in a biomanufacturing workflow. Nature Publishing Group UK 2017-07-27 /pmc/articles/PMC5532224/ /pubmed/28751635 http://dx.doi.org/10.1038/s41598-017-06949-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kwon, Taehong Prentice, Holly Oliveira, Jonas De Madziva, Nyasha Warkiani, Majid Ebrahimi Hamel, Jean-François P. Han, Jongyoon Microfluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture |
title | Microfluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture |
title_full | Microfluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture |
title_fullStr | Microfluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture |
title_full_unstemmed | Microfluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture |
title_short | Microfluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture |
title_sort | microfluidic cell retention device for perfusion of mammalian suspension culture |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5532224/ https://www.ncbi.nlm.nih.gov/pubmed/28751635 http://dx.doi.org/10.1038/s41598-017-06949-8 |
work_keys_str_mv | AT kwontaehong microfluidiccellretentiondeviceforperfusionofmammaliansuspensionculture AT prenticeholly microfluidiccellretentiondeviceforperfusionofmammaliansuspensionculture AT oliveirajonasde microfluidiccellretentiondeviceforperfusionofmammaliansuspensionculture AT madzivanyasha microfluidiccellretentiondeviceforperfusionofmammaliansuspensionculture AT warkianimajidebrahimi microfluidiccellretentiondeviceforperfusionofmammaliansuspensionculture AT hameljeanfrancoisp microfluidiccellretentiondeviceforperfusionofmammaliansuspensionculture AT hanjongyoon microfluidiccellretentiondeviceforperfusionofmammaliansuspensionculture |