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Test-retest reproducibility of cardiac magnetic resonance imaging in healthy mice at 7-Tesla: effect of anesthetic procedures

Cardiac magnetic resonance (CMR) has emerged as a powerful tool for in vivo assessments of cardiac parameters in experimental animal models of cardiovascular diseases, but its reproducibility in this setting remains poorly explored. To address this issue, we investigated the test-retest reproducibil...

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Autores principales: Joubert, Michael, Tager, Pia, Legallois, Damien, Defourneaux, Estelle, Le Guellec, Bastien, Gerber, Bernhard, Morello, Remy, Manrique, Alain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5532227/
https://www.ncbi.nlm.nih.gov/pubmed/28751730
http://dx.doi.org/10.1038/s41598-017-07083-1
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author Joubert, Michael
Tager, Pia
Legallois, Damien
Defourneaux, Estelle
Le Guellec, Bastien
Gerber, Bernhard
Morello, Remy
Manrique, Alain
author_facet Joubert, Michael
Tager, Pia
Legallois, Damien
Defourneaux, Estelle
Le Guellec, Bastien
Gerber, Bernhard
Morello, Remy
Manrique, Alain
author_sort Joubert, Michael
collection PubMed
description Cardiac magnetic resonance (CMR) has emerged as a powerful tool for in vivo assessments of cardiac parameters in experimental animal models of cardiovascular diseases, but its reproducibility in this setting remains poorly explored. To address this issue, we investigated the test-retest reproducibility of preclinical cardiac magnetic resonance imaging (CMR) at 7 Tesla in healthy C57BL/6 mice, including an analysis of the impact of different anesthetic procedures (isoflurane or pentobarbital). We also analyzed the intra-study reproducibility and the intra- and inter-observer post-processing reproducibility of CMR images. Test-retest reproducibility was high for left ventricular parameters, especially with the isoflurane anesthetic procedure, whereas right ventricular parameters and deformation measurements were less reproducible, mainly due to physiological variability. Post-processing reproducibility of CMR images was high both within and between observers. These results highlight that anesthetic procedures might influence CMR test-retest reproducibility, an important ethical consideration for longitudinal studies in rodent models of cardiomyopathy to limit the number of animals used.
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spelling pubmed-55322272017-08-02 Test-retest reproducibility of cardiac magnetic resonance imaging in healthy mice at 7-Tesla: effect of anesthetic procedures Joubert, Michael Tager, Pia Legallois, Damien Defourneaux, Estelle Le Guellec, Bastien Gerber, Bernhard Morello, Remy Manrique, Alain Sci Rep Article Cardiac magnetic resonance (CMR) has emerged as a powerful tool for in vivo assessments of cardiac parameters in experimental animal models of cardiovascular diseases, but its reproducibility in this setting remains poorly explored. To address this issue, we investigated the test-retest reproducibility of preclinical cardiac magnetic resonance imaging (CMR) at 7 Tesla in healthy C57BL/6 mice, including an analysis of the impact of different anesthetic procedures (isoflurane or pentobarbital). We also analyzed the intra-study reproducibility and the intra- and inter-observer post-processing reproducibility of CMR images. Test-retest reproducibility was high for left ventricular parameters, especially with the isoflurane anesthetic procedure, whereas right ventricular parameters and deformation measurements were less reproducible, mainly due to physiological variability. Post-processing reproducibility of CMR images was high both within and between observers. These results highlight that anesthetic procedures might influence CMR test-retest reproducibility, an important ethical consideration for longitudinal studies in rodent models of cardiomyopathy to limit the number of animals used. Nature Publishing Group UK 2017-07-27 /pmc/articles/PMC5532227/ /pubmed/28751730 http://dx.doi.org/10.1038/s41598-017-07083-1 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Joubert, Michael
Tager, Pia
Legallois, Damien
Defourneaux, Estelle
Le Guellec, Bastien
Gerber, Bernhard
Morello, Remy
Manrique, Alain
Test-retest reproducibility of cardiac magnetic resonance imaging in healthy mice at 7-Tesla: effect of anesthetic procedures
title Test-retest reproducibility of cardiac magnetic resonance imaging in healthy mice at 7-Tesla: effect of anesthetic procedures
title_full Test-retest reproducibility of cardiac magnetic resonance imaging in healthy mice at 7-Tesla: effect of anesthetic procedures
title_fullStr Test-retest reproducibility of cardiac magnetic resonance imaging in healthy mice at 7-Tesla: effect of anesthetic procedures
title_full_unstemmed Test-retest reproducibility of cardiac magnetic resonance imaging in healthy mice at 7-Tesla: effect of anesthetic procedures
title_short Test-retest reproducibility of cardiac magnetic resonance imaging in healthy mice at 7-Tesla: effect of anesthetic procedures
title_sort test-retest reproducibility of cardiac magnetic resonance imaging in healthy mice at 7-tesla: effect of anesthetic procedures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5532227/
https://www.ncbi.nlm.nih.gov/pubmed/28751730
http://dx.doi.org/10.1038/s41598-017-07083-1
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